2016
Stimulation of Slack K+ Channels Alters Mass at the Plasma Membrane by Triggering Dissociation of a Phosphatase-Regulatory Complex
Fleming MR, Brown MR, Kronengold J, Zhang Y, Jenkins DP, Barcia G, Nabbout R, Bausch AE, Ruth P, Lukowski R, Navaratnam DS, Kaczmarek LK. Stimulation of Slack K+ Channels Alters Mass at the Plasma Membrane by Triggering Dissociation of a Phosphatase-Regulatory Complex. Cell Reports 2016, 16: 2281-2288. PMID: 27545877, PMCID: PMC5123741, DOI: 10.1016/j.celrep.2016.07.024.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsBiosensing TechniquesBithionolBridged Bicyclo Compounds, HeterocyclicCell MembraneCerebral CortexFragile X Mental Retardation ProteinGene Expression RegulationHEK293 CellsHumansIon TransportMiceMice, KnockoutMicrofilament ProteinsMutationNerve Tissue ProteinsNeuronsPatch-Clamp TechniquesPhosphorylationPotassium ChannelsPotassium Channels, Sodium-ActivatedPrimary Cell CultureProtein BindingRNA, Small InterferingSignal TransductionThiazolidinesXenopus laevisConceptsProtein phosphatase 1Plasma membraneProtein kinase C.C-terminal residuesPhactr-1Potassium channelsPhosphatase 1Terminal domainSlack channelsHuman mutationsKinase C.Sodium-activated potassium channelsPharmacological activatorsOptical biosensor assayChannel stimulationSlack currentsBiosensor assaysMembraneMutantsPhosphorylationIntellectual disabilityProteinMutationsSevere intellectual disabilityActivator
2015
How Neurons Communicate: Gap Junctions and Neurosecretion
Levitan I, Kaczmarek L. How Neurons Communicate: Gap Junctions and Neurosecretion. 2015, 153-186. DOI: 10.1093/med/9780199773893.003.0008.ChaptersSynaptic vesiclesSNARE complex proteinsExistence of proteinsSecretion of neurotransmittersComplex proteinsPlasma membraneCytoplasmic vesiclesProteinVesiclesAdjacent cellsGap junctionsSmall moleculesCalcium entryExternal mediumElectrical couplingMembraneNeurosecretionCellsComplex processEndocytosisExocytosisFluorescent dyeMoleculesConnexinsCytoplasmReceptors and Transduction Mechanisms I: Receptors Coupled Directly to Ion Channels
Levitan I, Kaczmarek L. Receptors and Transduction Mechanisms I: Receptors Coupled Directly to Ion Channels. 2015, 239-262. DOI: 10.1093/med/9780199773893.003.0011.ChaptersSensory Receptors
Levitan I, Kaczmarek L. Sensory Receptors. 2015, 295-326. DOI: 10.1093/med/9780199773893.003.0013.Chapters
2013
The contribution of TWIK-1 channels to astrocyte K+ current is limited by retention in intracellular compartments
Wang W, Putra A, Schools GP, Ma B, Chen H, Kaczmarek LK, Barhanin J, Lesage F, Zhou M. The contribution of TWIK-1 channels to astrocyte K+ current is limited by retention in intracellular compartments. Frontiers In Cellular Neuroscience 2013, 7: 246. PMID: 24368895, PMCID: PMC3856854, DOI: 10.3389/fncel.2013.00246.Peer-Reviewed Original ResearchTWIK-1 channelsTWIK-1Plasma membraneIntracellular compartmentsMature hippocampal astrocytesTwo-pore domainWild-type animalsFractionation experimentsTwo-poreKnockout miceCytoplasmic fractionExpression of Kir4.1Compartment fractionsType animalsPhysiological conditionsTREK-1Whole-cell currentsLow-level presenceKnockoutLinear current-voltage relationshipCompartmentsMembraneMild effect
2008
PKC-Induced Intracellular Trafficking of CaV2 Precedes Its Rapid Recruitment to the Plasma Membrane
Zhang Y, Helm JS, Senatore A, Spafford JD, Kaczmarek LK, Jonas EA. PKC-Induced Intracellular Trafficking of CaV2 Precedes Its Rapid Recruitment to the Plasma Membrane. Journal Of Neuroscience 2008, 28: 2601-2612. PMID: 18322103, PMCID: PMC2830008, DOI: 10.1523/jneurosci.4314-07.Peer-Reviewed Original ResearchConceptsProtein kinase CActivation of PKCPlasma membraneGrowth conesLatrunculin BIntracellular traffickingActin polymerizationIntact microtubulesIntact actinKinase CChannel insertionPKC activationIon channelsMicrotubule polymerizationRapid recruitmentOrganellesLamellipodiumSubunitsMicrotubulesActinMembraneActivationRecruitmentCone terminalsNew sites
2004
Ion channels on intracellular organelles
Kaczmarek L, Jonas E. Ion channels on intracellular organelles. Advances In Molecular And Cell Biology 2004, 32: 433-458. DOI: 10.1016/s1569-2558(03)32018-1.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsExternal signalsIon channelsCellular functionsInner membraneMitochondrial channelsIntracellular membranesPlasma membraneIntracellular organellesMolecular identityCell deathCytoplasmic calcium levelsHandling of calciumRyanodine receptorProperties of channelsCytoplasmic calciumIntracellular storesPotential roleRelease of calciumInternal storesMembraneRegulationKey roleSynaptic transmissionCalcium influxIntracellular calcium
2003
Modulation of Synaptic Transmission by the BCL-2 Family Protein BCL-xL
Jonas EA, Hoit D, Hickman JA, Brandt TA, Polster BM, Fannjiang Y, McCarthy E, Montanez MK, Hardwick JM, Kaczmarek LK. Modulation of Synaptic Transmission by the BCL-2 Family Protein BCL-xL. Journal Of Neuroscience 2003, 23: 8423-8431. PMID: 12968005, PMCID: PMC6740692, DOI: 10.1523/jneurosci.23-23-08423.2003.Peer-Reviewed Original ResearchConceptsBcl-2 family proteinsProtein Bcl-xLBcl-xLFamily proteinsMitochondrial membranePro-apoptotic cleavage productRecombinant Bcl-xLBcl-xL proteinMitochondrial calcium uptakePresynaptic terminalsInfluences synaptic transmissionCell deathGiant presynaptic terminalSynaptic transmissionChannel activityProteinSquid stellate ganglionMitochondriaCleavage productsSynaptic stabilityAdult brainPostsynaptic responsesCalcium uptakeMembranePatch pipette
2001
Membrane Ion Channels and Ion Currents
B.Levitan I, Kaczmarek L. Membrane Ion Channels and Ion Currents. 2001, 67-88. DOI: 10.1093/oso/9780195145236.003.0004.Peer-Reviewed Original ResearchPlasma membraneTransmembrane ion flowIon flowMembrane ion channelsMovement of chargeExcellent electrical insulatorTransmembrane ionIon currentIon channelsLipid bilayersMembrane potentialHydrophobic interiorEndogenous electrical activityIonsElectrical insulatorSmall inorganic ionsMembraneTemporal patternsBilayersCellsExternal stimuliInsulatorNerve cellsSpeciesEnergyIon Channels, Membrane Ion Currents, and the Action Potential
B.Levitan I, Kaczmarek L. Ion Channels, Membrane Ion Currents, and the Action Potential. 2001, 113-138. DOI: 10.1093/oso/9780195145236.003.0006.Peer-Reviewed Original ResearchIon channelsPotassium channel proteinIon currentDetailed mechanistic understandingMembrane proteinsMolecular detailsActive ion channelsChannel proteinsBiological membranesMechanistic understandingSingle-channel recordingsProteinMembrane ion currentsVoltage-clamp measurementsMembraneChannel recordingsCellsMacroscopic currentsClamp measurementsCurrentElectrical phenomenaVoltage clampCurrent chapterMacroscopic membranesPotassium currentDiversity in the Structure and Function of Ion Channels
B.Levitan I, Kaczmarek L. Diversity in the Structure and Function of Ion Channels. 2001, 139-162. DOI: 10.1093/oso/9780195145236.003.0007.Peer-Reviewed Original ResearchIon channelsGreat diversityDiversityConsiderable diversityRapid membrane depolarizationMembrane depolarizationElectrical activityVoltage-dependent sodium currentsVoltage-dependent potassium currentsAxonal membraneSquid giant axonNeuronal cell bodiesCell bodiesGiant axonsPotassium currentAction potentialsSodium currentMembraneActivityAxonsHow Neurons Communicate: Gap Junctions and Neurosecretion
B.Levitan I, Kaczmarek L. How Neurons Communicate: Gap Junctions and Neurosecretion. 2001, 165-194. DOI: 10.1093/oso/9780195145236.003.0008.Peer-Reviewed Original Research
1999
Prolonged Activation of Mitochondrial Conductances During Synaptic Transmission
Jonas E, Buchanan J, Kaczmarek L. Prolonged Activation of Mitochondrial Conductances During Synaptic Transmission. Science 1999, 286: 1347-1350. PMID: 10558987, DOI: 10.1126/science.286.5443.1347.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsCalciumCalcium ChannelsDecapodiformesElectric ConductivityElectric StimulationIntracellular MembranesIon ChannelsIon TransportMicroscopy, ElectronMitochondriaPatch-Clamp TechniquesPorinsPresynaptic TerminalsSynaptic TransmissionTime FactorsVoltage-Dependent Anion ChannelsConceptsChannel activityIon channel activityMitochondrial membraneOnly organellesIntracellular organellesIntact cellsIon channelsMitochondriaOrganellesLarge conductanceTens of secondsPresynaptic terminalsIon transportSynaptic transmissionSynaptic stimulationConductanceElectron microscopyPatch-clamp techniqueMembraneActivityCellsActivationSquidStimulation
1998
Protein Kinase C Regulates a Vesicular Class of Calcium Channels in the Bag Cell Neurons of Aplysia
White B, Nick T, Carew T, Kaczmarek L. Protein Kinase C Regulates a Vesicular Class of Calcium Channels in the Bag Cell Neurons of Aplysia. Journal Of Neurophysiology 1998, 80: 2514-2520. PMID: 9819259, DOI: 10.1152/jn.1998.80.5.2514.Peer-Reviewed Original Research
1997
Giga-Ohm Seals on Intracellular Membranes: A Technique for Studying Intracellular Ion Channels in Intact Cells
Jonas E, Knox R, Kaczmarek L. Giga-Ohm Seals on Intracellular Membranes: A Technique for Studying Intracellular Ion Channels in Intact Cells. Neuron 1997, 19: 7-13. PMID: 9247259, DOI: 10.1016/s0896-6273(00)80343-8.Peer-Reviewed Original ResearchConceptsGiga-ohm sealsIntracellular membranesIntact cellsIntracellular ion channelsMembrane ion channel activityIon channel activityConventional patch clampingOrganelle membranesPlasma membraneInternal organellesInternal membranesInternal organelles membraneCell typesIon channelsChannel activityConfocal imagingInositol trisphosphateLipophilic fluorescent dyeMembranePatch clampingConcentric electrode arrangementPatch-clamp techniqueCellsIntact neuronsOrganelles