The distribution of Na+,K+-ATPase in the retinal pigmented epithelium from chicken embryo is polarized in vivo but not in primary cell culture
Rizzolo L. The distribution of Na+,K+-ATPase in the retinal pigmented epithelium from chicken embryo is polarized in vivo but not in primary cell culture. Experimental Eye Research 1990, 51: 435-446. PMID: 2170160, DOI: 10.1016/0014-4835(90)90156-o.Peer-Reviewed Original ResearchConceptsBasolateral plasma membranePlasma membranePrimary cell culturesPlasma membrane proteinsFunctional tight junctionsCultured RPEChicken embryosMatrix-coated filtersExtracellular matrix coatingCell culturesMembrane proteinsVesicular stomatitis virusMorphological polarityApical poleCultured cellsApical membraneEngelbreth-HolmJunctional complexesSwarm tumorEmbryonic eyeStomatitis virusATPaseBasolateral membraneSerum-free mediumTight junctions