P148 Uncovering antibodies to cryptic epitopes: Use of flow cytometry crossmatch to differentiate between functional and denatured epitopes
Mangiola M, Lomago J, Marilyn M, Nichol L, Zern D, McGowan K, Sese D, Bow L, Zeevi A. P148 Uncovering antibodies to cryptic epitopes: Use of flow cytometry crossmatch to differentiate between functional and denatured epitopes. Human Immunology 2016, 77: 145. DOI: 10.1016/j.humimm.2016.07.213.Peer-Reviewed Original ResearchAntigen beadsCryptic epitopesFlow cytometry crossmatchSingle antigen beadsSmall bowel transplantHLA antibody detectionClass II profileBowel transplantCytometry crossmatchDR antigensHLA AbsDR7 allelesPatient beingCrossmatch requestsAntibody detectionSurrogate donorsMolecular typingFlow cytometryExon 2Ab profilesPotential targetPatient cellsEpitopesPatientsPremature stop codonP002 The consistent inconsistency in HLA antibody testing practices: a collaboration with the Banff workgroup
Dadhania D, Jackson A, Campbell P, Bow L, Almeshari K, Schinstock C, Cornell L, Bagnasco S, Kraus E, Cozzi E, Askar M. P002 The consistent inconsistency in HLA antibody testing practices: a collaboration with the Banff workgroup. Human Immunology 2016, 77: 41. DOI: 10.1016/j.humimm.2016.07.067.Peer-Reviewed Original ResearchDonor-specific HLA antibodiesSingle antigen beadsHLA AbsAntigen beadsNon-sensitized patientsSpecific HLA antibodiesLarge transplant centerDifferent practice patternsDiagnosis of antibodyBanff criteriaHLA antibodiesSensitization statusSurveillance biopsiesPatients 54Transplant centersCombination of assaysPatient outcomesStrong antibodyPractice patternsPatient variesTesting practicesClinical relevanceHistocompatibility laboratoriesProzone effectAntibodies