2023
Cell Surface RNAs Control Neutrophil Function
Zhang N, Tang W, Torres L, Zhu L, Wang X, Ajaj Y, Wang Y, Zhang D, Kurbatov V, Zhou H, Luan Y, Kumar P, Hidalgo A, Wu D, Lu J. Cell Surface RNAs Control Neutrophil Function. Blood 2023, 142: 674. DOI: 10.1182/blood-2023-187570.Peer-Reviewed Original ResearchExtracellular RNaseCell surfaceTotal RNABona fide ligandsEndothelial cellsOuter cell surfaceTransendothelial migrationMammalian cellsSuch RNAsGlycan modificationsCellular RNAGlycoRNARNase digestionLive cellsRNAHematopoietic cellsRNase treatmentSimilar defectsIntegrin levelsConfocal microscopyRNaseGlycan fractionImportant functionsHomologuesRecombinant E-selectin
2020
Resolving Cell Cycle Speed in One Snapshot with a Live-Cell Fluorescent Reporter
Eastman AE, Chen X, Hu X, Hartman AA, Morales A, Yang C, Lu J, Kueh HY, Guo S. Resolving Cell Cycle Speed in One Snapshot with a Live-Cell Fluorescent Reporter. Cell Reports 2020, 31: 107804. PMID: 32579930, PMCID: PMC7418154, DOI: 10.1016/j.celrep.2020.107804.Peer-Reviewed Original ResearchConceptsFluorescent reportersLive-cell fluorescent reporterCell cycle speedFluorescent timer proteinsCell proliferationCell cycle dynamicsRed fluorescent proteinFaster cycling cellsFate transitionsFusion reporterActive lociTimer proteinFluorescent proteinLength heterogeneityComplex tissuesHematopoietic cellsCycling cellsReporterFluorescence ratioCycle dynamicsProteinFunctional heterogeneityMouse strainsSolid tissuesCycle speed
2009
Regulation of mir-196b by MLL and its overexpression by MLL fusions contributes to immortalization
Popovic R, Riesbeck LE, Velu CS, Chaubey A, Zhang J, Achille NJ, Erfurth FE, Eaton K, Lu J, Grimes HL, Chen J, Rowley JD, Zeleznik-Le NJ. Regulation of mir-196b by MLL and its overexpression by MLL fusions contributes to immortalization. Blood 2009, 113: 3314-3322. PMID: 19188669, PMCID: PMC2665896, DOI: 10.1182/blood-2008-04-154310.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell DifferentiationCell ProliferationCell Transformation, NeoplasticCells, CulturedEmbryonic Stem CellsGene Expression RegulationHistone-Lysine N-MethyltransferaseLeukemiaMiceMice, Inbred C57BLMicroRNAsMolecular Sequence DataMyeloid-Lymphoid Leukemia ProteinRecombinant Fusion ProteinsSequence Homology, Nucleic AcidUp-RegulationConceptsMLL fusion proteinsHox genesMiR-196bLeukemogenic MLL fusion proteinsFusion proteinEmbryonic stem cell differentiationStem cell differentiationDifferentiated hematopoietic cellsShort-term hematopoietic stem cellsMixed lineage leukemia (MLL) geneBone marrow progenitor cellsLeukemia developmentHOXA clusterHematopoietic stem cellsPrimary leukemia samplesChimeric proteinMarrow progenitor cellsHematopoietic lineagesCell differentiationLeukemia geneFusion contributesChromosomal translocationsHematopoietic cellsGenesStem cells
1999
Rat NAP1: cDNA cloning and upregulation by Mpl ligand
Cataldo L, Zhang Y, Lu J, Ravid K. Rat NAP1: cDNA cloning and upregulation by Mpl ligand. Gene 1999, 226: 355-364. PMID: 9931510, DOI: 10.1016/s0378-1119(98)00563-0.Peer-Reviewed Original ResearchMeSH KeywordsAgingAmino Acid SequenceAnimalsBase SequenceBone Marrow CellsCell Cycle ProteinsCell LineageCells, CulturedCloning, MolecularDNA, ComplementaryGene Expression RegulationMolecular Sequence DataNuclear ProteinsNucleosome Assembly Protein 1ProteinsRatsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSequence Homology, Amino AcidThrombopoietinUp-RegulationConceptsMpl ligandNucleosome assembly proteinPrimary bone marrow cellsFull-length cDNASize classesFormation of nucleosomesC-Mpl receptorChromatin reorganizationMultiple size classesAssembly proteinPolymerase chain reaction approachRelaxed circular DNACDNA cloningKb cDNAHematopoietic lineagesKb mRNARecombinant proteinsHematopoietic cytokinesHematopoietic cellsCircular DNAPlatelet precursorsBone marrow cellsHematopoietic tissuesCell linesCDNA