2011
Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexaconfers enhanced adhesion to cultured cells and fibronectin
Figueira CP, Croda J, Choy HA, Haake DA, Reis MG, Ko AI, Picardeau M. Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexaconfers enhanced adhesion to cultured cells and fibronectin. BMC Microbiology 2011, 11: 129. PMID: 21658265, PMCID: PMC3133549, DOI: 10.1186/1471-2180-11-129.Peer-Reviewed Original ResearchConceptsHeterologous expressionL. biflexaFunctional genetic analysisCultured cellsPathogen-specific proteinsVirulence-associated phenotypesSaprophytic L. biflexaPathogenesis of leptospirosisImmunoglobulin-like proteinEukaryotic cellsKey virulence factorsExtracellular matrix componentsLeptospiral immunoglobulin-like (Lig) proteinsSurrogate hostLig geneLeptospira biflexa serovar patocMolecular basisReplicative plasmidGenetic analysisLig proteinsSurface localizationCell adhesionSurface proteinsVirulence factorsLigB
2008
Genome-Wide Transposon Mutagenesis in Pathogenic Leptospira Species
Murray GL, Morel V, Cerqueira GM, Croda J, Srikram A, Henry R, Ko AI, Dellagostin OA, Bulach DM, Sermswan RW, Adler B, Picardeau M. Genome-Wide Transposon Mutagenesis in Pathogenic Leptospira Species. Infection And Immunity 2008, 77: 810-816. PMID: 19047402, PMCID: PMC2632054, DOI: 10.1128/iai.01293-08.Peer-Reviewed Original ResearchConceptsTransposon mutagenesisTransposon mutantsGenome-wide transposon mutagenesisL. interrogansTransposon insertion sitesNovel virulence factorsEssential genesHypothetical genesGene functionGenome sequenceTransposon insertionGene disruptionGenetic manipulationDifferent genesGenetic analysisHeme biosynthesisPathogenic Leptospira speciesMutantsSequence analysisGenesVirulence factorsInsertional hot spotsLocation of insertionGenomeMutagenesisTargeted Mutagenesis in Pathogenic Leptospira Species: Disruption of the LigB Gene Does Not Affect Virulence in Animal Models of Leptospirosis
Croda J, Figueira CP, Wunder EA, Santos CS, Reis MG, Ko AI, Picardeau M. Targeted Mutagenesis in Pathogenic Leptospira Species: Disruption of the LigB Gene Does Not Affect Virulence in Animal Models of Leptospirosis. Infection And Immunity 2008, 76: 5826-5833. PMID: 18809657, PMCID: PMC2583567, DOI: 10.1128/iai.00989-08.Peer-Reviewed Original ResearchConceptsPathogenic Leptospira speciesLeptospira speciesAcute disease manifestationsPersistent renal colonizationInoculation of ratsDisease manifestationsHamster modelRenal colonizationPathogenic mechanismsAnimal modelsPersistent colonizationDecrease virulenceLeptospira interrogansHomologous recombinationLeptospirosisVirulence factorsBacterial adherenceImmunoblot analysisCorresponding chromosomal locusWild-type strainCultured cellsFirst evidenceSpectinomycin resistance geneRepeat proteinsTargeted Mutagenesis
2007
The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis
Silva É, Medeiros MA, McBride AJ, Matsunaga J, Esteves GS, Ramos JG, Santos CS, Croda J, Homma A, Dellagostin OA, Haake DA, Reis MG, Ko AI. The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis. Vaccine 2007, 25: 6277-6286. PMID: 17629368, PMCID: PMC1994161, DOI: 10.1016/j.vaccine.2007.05.053.Peer-Reviewed Original ResearchConceptsMajor public health problemRobust antibody responseGolden Syrian hamstersPublic health problemPotential intervention strategiesLethal inoculumSurface-exposed determinantsProtective immunityAntibody responseSterilizing immunityLethal infectionHamster modelVaccine candidatesSubunit vaccineCompanion animalsHealth problemsVeterinary diseasesSyrian hamstersLeptospiral immunoglobulin-like (Lig) proteinsLeptospira interrogansLeptospirosisL. interrogansIntervention strategiesVirulence factorsImmunoglobulin-like protein