Investigation of Preanalytical Variables Impacting Pathogen Cell-Free DNA in Blood and Urine
Murugesan K, Hogan C, Palmer Z, Reeve B, Theron G, Andama A, Somoskovi A, Steadman A, Madan D, Andrews J, Croda J, Sahoo M, Cattamanchi A, Pinsky B, Banaei N. Investigation of Preanalytical Variables Impacting Pathogen Cell-Free DNA in Blood and Urine. Journal Of Clinical Microbiology 2019, 57: 10.1128/jcm.00782-19. PMID: 31511335, PMCID: PMC6813001, DOI: 10.1128/jcm.00782-19.Peer-Reviewed Original ResearchConceptsEpstein-Barr virusCell-free DNABlood collection tubesUrine samplesLower medianPCR cycle thresholdCollection tubesEDTA blood samplesTB patientsTuberculosis patientsUrine volumeHealthy donorsBlood samplesAttractive biomarkerEDTA tubesPatient samplesBloodWhole urineThawed plasmaUrineUrine preservativeCycle thresholdMedianPatientsPreanalytical factorsDetection, survival and infectious potential of Mycobacterium tuberculosis in the environment: a review of the evidence and epidemiological implications
Martinez L, Verma R, Croda J, Horsburgh C, Walter K, Degner N, Middelkoop K, Koch A, Hermans S, Warner D, Wood R, Cobelens F, Andrews J. Detection, survival and infectious potential of Mycobacterium tuberculosis in the environment: a review of the evidence and epidemiological implications. European Respiratory Journal 2019, 53: 1802302. PMID: 31048345, PMCID: PMC6753378, DOI: 10.1183/13993003.02302-2018.Peer-Reviewed Original ResearchConceptsInfectious tuberculosis patientsTuberculosis patientsTB transmissionCongregate settingsMouse modelGuinea pigsInfectious threatsMycobacterium tuberculosisDroplet nucleiConsistent findingScientific evidenceInfectious potentialEpidemiological implicationsPrimary routeFurther investigationSeminal experimental studiesRecent studiesHuman sourcesEvidencePatientsTuberculosisFindingsStudyInfectiousnessViability