2014
Chloride-driven Electromechanical Phase Lags at Acoustic Frequencies Are Generated by SLC26a5, the Outer Hair Cell Motor Protein
Santos-Sacchi J, Song L. Chloride-driven Electromechanical Phase Lags at Acoustic Frequencies Are Generated by SLC26a5, the Outer Hair Cell Motor Protein. Biophysical Journal 2014, 107: 126-133. PMID: 24988347, PMCID: PMC4119270, DOI: 10.1016/j.bpj.2014.05.018.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MovementChloridesGuinea PigsHair Cells, Auditory, OuterMembrane PotentialsProtein BindingProtein Structure, TertiaryProteinsReaction TimeSound
2005
On the Effect of Prestin on the Electrical Breakdown of Cell Membranes
Navarrete EG, Santos-Sacchi J. On the Effect of Prestin on the Electrical Breakdown of Cell Membranes. Biophysical Journal 2005, 90: 967-974. PMID: 16299081, PMCID: PMC1367121, DOI: 10.1529/biophysj.105.064105.Peer-Reviewed Original ResearchConceptsTSA cellsWhole cell levelVoltage-dependent activityGuinea pig OHCsOuter hair cell motor proteinMotor proteinsPM domainsMaximum transmembrane potentialPrestin activityMembrane susceptibilityTransmembrane potentialCell membranePrestinTsA201 cellsBiophysical influencesMembrane stiffnessElectroporationCellsBasal portionSignificant resistanceMembrane capacitanceProteinElectromotilityAuditory sensitivitySusceptibilityN-Terminal-Mediated Homomultimerization of Prestin, the Outer Hair Cell Motor Protein
Navaratnam D, Bai JP, Samaranayake H, Santos-Sacchi J. N-Terminal-Mediated Homomultimerization of Prestin, the Outer Hair Cell Motor Protein. Biophysical Journal 2005, 89: 3345-3352. PMID: 16113116, PMCID: PMC1366831, DOI: 10.1529/biophysj.105.068759.Peer-Reviewed Original ResearchMeSH KeywordsAllosteric SiteAmino Acid SequenceAnimalsCell MembraneCell SeparationCHO CellsCricetinaeDimerizationElectric CapacitanceElectric ConductivityElectrophysiologyFlow CytometryFluorescence Resonance Energy TransferGerbillinaeGreen Fluorescent ProteinsHair Cells, Auditory, OuterMechanotransduction, CellularMembrane PotentialsMicroscopy, ConfocalMicroscopy, FluorescenceModels, BiologicalMolecular Motor ProteinsMolecular Sequence DataMutationProtein BindingProtein ConformationProtein Structure, TertiaryProteinsTransfectionConceptsOuter Hair Cell Motor ProteinStructure-function relationsNormal membrane targetingMammalian cochlear amplificationIntact N-terminusMembrane targetingDomain proteinsMembrane topologyFluorescence resonance energy transferCell mechanical responsePrestin moleculesMembrane motorMotor proteinsN-terminusShort truncationsPrestinResonance energy transferIntracellular terminiAllosteric modulationProteinTerminusCochlear amplificationVoltage sensingHomomultimerizationModified activity
2002
Functional motor microdomains of the outer hair cell lateral membrane
Santos-Sacchi J. Functional motor microdomains of the outer hair cell lateral membrane. Pflügers Archiv - European Journal Of Physiology 2002, 445: 331-336. PMID: 12466934, DOI: 10.1007/s00424-002-0928-4.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneElectric CapacitanceElectrophysiologyGuinea PigsHair Cells, Auditory, OuterIon Channel GatingPatch-Clamp TechniquesProtein Structure, TertiaryProteinsConceptsOperating voltage rangeLateral membranesMammalian inner earOuter hair cell lateral membraneOuter hair cellsMotor proteinsFunctional variationMechanical deformationNeuroepithelial cellsVoltage rangeMicrodomainsWhole cellsMidpoint voltageHair cellsMotor chargeCell characteristicsMembraneCellsNonlinear capacitanceInner ear