2009
Proteomic-Based Detection of a Protein Cluster Dysregulated during Cardiovascular Development Identifies Biomarkers of Congenital Heart Defects
Nath AK, Krauthammer M, Li P, Davidov E, Butler LC, Copel J, Katajamaa M, Oresic M, Buhimschi I, Buhimschi C, Snyder M, Madri JA. Proteomic-Based Detection of a Protein Cluster Dysregulated during Cardiovascular Development Identifies Biomarkers of Congenital Heart Defects. PLOS ONE 2009, 4: e4221. PMID: 19156209, PMCID: PMC2626248, DOI: 10.1371/journal.pone.0004221.Peer-Reviewed Original ResearchConceptsCardiovascular developmentMass spectrometry-based proteomicsSpectrometry-based proteomicsNormal cardiovascular developmentAdhesion/migrationHuman CHDsProteomic datasetsHeart developmentProtein clustersMurine embryosProtein pathwayMolecular pathwaysFunctional roleProteinEmbryonic survivalYolk sacProtein levelsIdentifies biomarkersCardiovascular defectsWestern blottingPathwayNovel avenuesEmbryosComplete understandingProtein biomarkers
2004
Nitric oxide modulates murine yolk sac vasculogenesis and rescues glucose induced vasculopathy
Nath AK, Enciso J, Kuniyasu M, Hao XY, Madri JA, Pinter E. Nitric oxide modulates murine yolk sac vasculogenesis and rescues glucose induced vasculopathy. Development 2004, 131: 2485-2496. PMID: 15128676, DOI: 10.1242/dev.01131.Peer-Reviewed Original ResearchConceptsVascular developmentReactive oxygen speciesYolk sac vasculogenesisMurine yolk sacYolk sacBlood island formationEnvironmental insultsNormal embryonic growthMurine embryo culturePreimplantation embryogenesisAbnormal vascular developmentGenetic manipulationDevelopmental arrestExpression patternsPostimplantation developmentNitric oxideEndodermal layerEmbryonic growthFunctional inactivationHigh glucoseROS productionRole of NOBlastocyst invasionProtein levelsEmbryo culture
2003
Elevated glucose inhibits VEGF-A–mediated endocardial cushion formation
Enciso JM, Gratzinger D, Camenisch TD, Canosa S, Pinter E, Madri JA. Elevated glucose inhibits VEGF-A–mediated endocardial cushion formation. Journal Of Cell Biology 2003, 160: 605-615. PMID: 12591918, PMCID: PMC2173755, DOI: 10.1083/jcb.200209014.Peer-Reviewed Original ResearchMeSH KeywordsAngiogenesis Inducing AgentsAnimalsCell MovementCell SizeCells, CulturedCulture TechniquesDipeptidesEmbryo, MammalianEndocardial Cushion DefectsFemaleGlucoseHeartMaleMatrix Metalloproteinase 2MiceMorphogenesisMyocardiumPlatelet Endothelial Cell Adhesion Molecule-1Protease InhibitorsRecombinant Fusion ProteinsVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-1ConceptsEpithelial-mesenchymal transformationEndocardial cushion formationPlatelet endothelial cell adhesion molecule-1Cushion formationPECAM-1-positive endothelial cellsSingle cell motilityMMP-2 expressionMorphogenesis resultsHigh glucose-induced inhibitionCell motilityEndothelial cellsBlocks invasionMatrix metalloproteinase-2 expressionEndocardial cellsExtracellular matrixLack of invasionEndothelial cell adhesion molecule-1Mesenchymal cellsMyocardial VEGFMMP-2 inductionMetalloproteinase-2 expressionVEGF-A165ExpressionGrowth factorVascular endothelial growth factor
1999
Hyperglycemia-Induced Vasculopathy in the Murine Vitelline Vasculature Correlation with PECAM-1/CD31 Tyrosine Phosphorylation State
Pinter E, Mahooti S, Wang Y, Imhof B, Madri J. Hyperglycemia-Induced Vasculopathy in the Murine Vitelline Vasculature Correlation with PECAM-1/CD31 Tyrosine Phosphorylation State. American Journal Of Pathology 1999, 154: 1367-1379. PMID: 10329590, PMCID: PMC1866605, DOI: 10.1016/s0002-9440(10)65391-6.Peer-Reviewed Original ResearchConceptsPECAM-1 tyrosine phosphorylationTyrosine phosphorylation stateMurine conceptusYolk sacCell-extracellular matrixFirst organ systemCell-factor interactionsTyrosine dephosphorylationEndothelial cell migrationEmbryonic angioblastsEmbryonic developmentMesodermal cellsVasculogenesis/angiogenesisTyrosine phosphorylationPhosphorylation statePerinatal lethalityNormal organogenesisVascular systemCell migrationHematopoietic cellsDiabetic pregnant miceComplex vascular systemVasculogenesisPECAM-1 expressionOffspring