2002
Semireannealing, Single-Stranded Conformational Polymorphism: A Novel and Effective Tool for the Diagnosis of T-Cell Clonality
Crisi G, Emanuel J, Johnson C, Crotty P, Costa J, Tallini G. Semireannealing, Single-Stranded Conformational Polymorphism: A Novel and Effective Tool for the Diagnosis of T-Cell Clonality. Applied Immunohistochemistry & Molecular Morphology 2002, 11: 67-74. PMID: 12045709, DOI: 10.1097/00019606-200206000-00002.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceClone CellsDNA, NeoplasmElectrophoresis, CapillaryElectrophoresis, Polyacrylamide GelGene RearrangementHumansLymph NodesLymphoma, B-CellLymphoma, T-CellMolecular Sequence DataNucleic Acid ConformationPolymerase Chain ReactionPolymorphism, Single-Stranded ConformationalPseudolymphomaReceptors, Antigen, T-Cell, gamma-deltaSequence AlignmentSequence Analysis, DNAp53 Mutations as Tumor Markers in Fine Needle Aspirates of Palpable Breast Masses
Dillon DA, Hipolito E, Zheng K, Rimm DL, Costa JC. p53 Mutations as Tumor Markers in Fine Needle Aspirates of Palpable Breast Masses. Acta Cytologica 2002, 46: 841-847. PMID: 12365217, DOI: 10.1159/000327057.Peer-Reviewed Original ResearchMeSH KeywordsAbscessAdenocarcinomaAdultAgedAged, 80 and overAmino Acid SubstitutionBiomarkers, TumorBiopsy, NeedleBreast NeoplasmsCarcinoma, Ductal, BreastCodon, TerminatorCystsDNA Mutational AnalysisDNA, NeoplasmExonsFemaleFrameshift MutationGenes, p53GenotypeHumansMiddle AgedMutationPolymorphism, Single-Stranded ConformationalRetrospective StudiesConceptsFine needle aspiratesP53 exons 5Breast massesPolymerase chain reactionNeedle aspiratesP53 mutationsSingle-strand conformational polymorphism analysisSubsequent excisional biopsyPalpable breast massesPotential diagnostic utilityDefinitive cytologic diagnosisTumor cell markersExon 5Molecular diagnostic markersExcisional biopsyBenign cytologyBreast carcinomaSuspicious cytologyRetrospective analysisCytologic diagnosisTumor markersDiagnostic criteriaBiopsy tissueMorphologic diagnosisDiagnostic utility
2001
Multiplex Detection of Hotspot Mutations by Rolling Circle-Enabled Universal Microarrays
Ladner D, Leamon J, Hamann S, Tarafa G, Strugnell T, Dillon D, Lizardi P, Costa J. Multiplex Detection of Hotspot Mutations by Rolling Circle-Enabled Universal Microarrays. Laboratory Investigation 2001, 81: 1079-1086. PMID: 11502859, DOI: 10.1038/labinvest.3780320.Peer-Reviewed Original ResearchDetection of the Mutated K-Ras Biomarker in Colorectal Carcinoma
Doolittle B, Emanuel J, Tuttle C, Costa J. Detection of the Mutated K-Ras Biomarker in Colorectal Carcinoma. Experimental And Molecular Pathology 2001, 70: 289-301. PMID: 11418008, DOI: 10.1006/exmp.2001.2364.Peer-Reviewed Original ResearchMeSH KeywordsBiomarkers, TumorColorectal NeoplasmsDeoxyribonuclease EcoRIDNADNA, NeoplasmFecesGenes, rasHumansIndicators and ReagentsMutationPolymerase Chain ReactionPolymorphism, Single-Stranded ConformationalQuaternary Ammonium CompoundsReference ValuesReproducibility of ResultsSite-Specific DNA-Methyltransferase (Adenine-Specific)Trimethyl Ammonium CompoundsConceptsColorectal carcinomaStool samplesHealthy patientsK-rasReliable screening testDouble-blind formatK-ras mutationsWild-type K-rasK-ras oncogeneK-ras sequencesCommon malignancyCRC tumorsPolymerase chain reactionEarly lesionsStool DNANoninvasive screenScreening testTumor tissueGenetic biomarkersHuman stoolPatientsEarly detectionHigh false positive rateSingle-strand conformation polymorphismTissue DNARapid, Efficient Genotyping of Clinical Tumor Samples by Laser-Capture Microdissection/PCR/SSCP
Dillon D, Zheng K, Costa J. Rapid, Efficient Genotyping of Clinical Tumor Samples by Laser-Capture Microdissection/PCR/SSCP. Experimental And Molecular Pathology 2001, 70: 195-200. PMID: 11417998, DOI: 10.1006/exmp.2001.2362.Peer-Reviewed Original ResearchConceptsLaser capture microdissectionP53 exons 5PCR/SSCPTumor genotypingPure tumor cell populationsSSCP/sequencingSurgical pathology reportsRoutine clinical useClinical tumor samplesExon 5Colorectal adenocarcinomaTherapeutic responsePathology reportsTumor cell populationLaser-microdissected samplesClinical practiceTumor samplesClinical useSporadic mutationsExon 1Molecular analysisCell populationsDirect sequencingMutation analysisPCR products
1996
Identification of mutations in the Ki-ras gene in human retinoblastoma.
Bautista D, Emanuel J, Granville C, Howard R, Costa J. Identification of mutations in the Ki-ras gene in human retinoblastoma. Investigative Ophthalmology & Visual Science 1996, 37: 2313-7. PMID: 8843915.Peer-Reviewed Original ResearchConceptsKi-ras geneKi-rasIdentification of mutationsSelective growth advantageGenomic sequencesSingle-strand conformation polymorphismGrowth advantageTumor cell populationGenesExon 1Ki-ras mutationsMutationsExon 2Cell populationsConformation polymorphismHuman retinoblastomaClonal mutationsDirect sequencingPolymerase chain reactionTumor samplesLight microscopyMutational statusChain reactionRetinoblastomaOncogene
1992
Induction of apoptosis by wild-type p53 in a human colon tumor-derived cell line.
Shaw P, Bovey R, Tardy S, Sahli R, Sordat B, Costa J. Induction of apoptosis by wild-type p53 in a human colon tumor-derived cell line. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 4495-4499. PMID: 1584781, PMCID: PMC49109, DOI: 10.1073/pnas.89.10.4495.Peer-Reviewed Original ResearchStability of K-ras mutations throughout the natural history of human colorectal cancer
Losi L, Benhattar J, Costa J. Stability of K-ras mutations throughout the natural history of human colorectal cancer. European Journal Of Cancer 1992, 28: 1115-1120. PMID: 1627381, DOI: 10.1016/0959-8049(92)90468-h.Peer-Reviewed Original ResearchConceptsK-ras mutationsPrimary tumorRas mutationsNatural historyColorectal cancerTumor markersStable tumor markerHuman colorectal cancerDistant recurrenceRecurrent cancerRecurrent tumorsPolymerase chain reactionColorectal carcinomaCodon 12TumorsArchival tissueCancerChain reactionAllele-specific amplificationPatientsRecurrenceMarkersMutationsCarcinomaDisease