2002
p53 Mutations as Tumor Markers in Fine Needle Aspirates of Palpable Breast Masses
Dillon DA, Hipolito E, Zheng K, Rimm DL, Costa JC. p53 Mutations as Tumor Markers in Fine Needle Aspirates of Palpable Breast Masses. Acta Cytologica 2002, 46: 841-847. PMID: 12365217, DOI: 10.1159/000327057.Peer-Reviewed Original ResearchMeSH KeywordsAbscessAdenocarcinomaAdultAgedAged, 80 and overAmino Acid SubstitutionBiomarkers, TumorBiopsy, NeedleBreast NeoplasmsCarcinoma, Ductal, BreastCodon, TerminatorCystsDNA Mutational AnalysisDNA, NeoplasmExonsFemaleFrameshift MutationGenes, p53GenotypeHumansMiddle AgedMutationPolymorphism, Single-Stranded ConformationalRetrospective StudiesConceptsFine needle aspiratesP53 exons 5Breast massesPolymerase chain reactionNeedle aspiratesP53 mutationsSingle-strand conformational polymorphism analysisSubsequent excisional biopsyPalpable breast massesPotential diagnostic utilityDefinitive cytologic diagnosisTumor cell markersExon 5Molecular diagnostic markersExcisional biopsyBenign cytologyBreast carcinomaSuspicious cytologyRetrospective analysisCytologic diagnosisTumor markersDiagnostic criteriaBiopsy tissueMorphologic diagnosisDiagnostic utility
2001
Detection of the Mutated K-Ras Biomarker in Colorectal Carcinoma
Doolittle B, Emanuel J, Tuttle C, Costa J. Detection of the Mutated K-Ras Biomarker in Colorectal Carcinoma. Experimental And Molecular Pathology 2001, 70: 289-301. PMID: 11418008, DOI: 10.1006/exmp.2001.2364.Peer-Reviewed Original ResearchMeSH KeywordsBiomarkers, TumorColorectal NeoplasmsDeoxyribonuclease EcoRIDNADNA, NeoplasmFecesGenes, rasHumansIndicators and ReagentsMutationPolymerase Chain ReactionPolymorphism, Single-Stranded ConformationalQuaternary Ammonium CompoundsReference ValuesReproducibility of ResultsSite-Specific DNA-Methyltransferase (Adenine-Specific)Trimethyl Ammonium CompoundsConceptsColorectal carcinomaStool samplesHealthy patientsK-rasReliable screening testDouble-blind formatK-ras mutationsWild-type K-rasK-ras oncogeneK-ras sequencesCommon malignancyCRC tumorsPolymerase chain reactionEarly lesionsStool DNANoninvasive screenScreening testTumor tissueGenetic biomarkersHuman stoolPatientsEarly detectionHigh false positive rateSingle-strand conformation polymorphismTissue DNA
1999
Analysis of K-ras, N-ras, H-ras, and p53 in Lung Neuroendocrine Neoplasms
Couce M, Bautista D, Costa J, Carter D. Analysis of K-ras, N-ras, H-ras, and p53 in Lung Neuroendocrine Neoplasms. Applied Immunohistochemistry & Molecular Morphology 1999, 8: 71-79. PMID: 10475381, DOI: 10.1097/00019606-199906000-00003.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAllelesDNA Mutational AnalysisExonsFemaleGenes, rasHumansImmunohistochemistryLung NeoplasmsMaleMiddle AgedMutation, MissenseNeuroendocrine TumorsPoint MutationPolymerase Chain ReactionPolymorphism, GeneticPolymorphism, Single-Stranded ConformationalRetrospective StudiesTumor Suppressor Protein p53ConceptsLarge cell neuroendocrine carcinomaPulmonary neuroendocrine tumorsAtypical carcinoidNeuroendocrine tumorsTypical carcinoidP53 geneK-rasCell neuroendocrine carcinomaSingle-strand conformation polymorphismHigh-grade tumorsHigh-grade carcinomaH-ras mutationsLung neuroendocrine neoplasmsN-rasK-ras mutationsH-RasExon 5Neuroendocrine carcinomaPolymerase chain reactionNeuroendocrine neoplasmsPositive immunostainingGenotypic mutationsN-ras geneTumors
1996
Identification of mutations in the Ki-ras gene in human retinoblastoma.
Bautista D, Emanuel J, Granville C, Howard R, Costa J. Identification of mutations in the Ki-ras gene in human retinoblastoma. Investigative Ophthalmology & Visual Science 1996, 37: 2313-7. PMID: 8843915.Peer-Reviewed Original ResearchConceptsKi-ras geneKi-rasIdentification of mutationsSelective growth advantageGenomic sequencesSingle-strand conformation polymorphismGrowth advantageTumor cell populationGenesExon 1Ki-ras mutationsMutationsExon 2Cell populationsConformation polymorphismHuman retinoblastomaClonal mutationsDirect sequencingPolymerase chain reactionTumor samplesLight microscopyMutational statusChain reactionRetinoblastomaOncogeneDetection of rare circulating human colon tumor cells in a nude mouse xenograft model
Pages J, Sordat B, Bautista D, Costa J, Benhattar J. Detection of rare circulating human colon tumor cells in a nude mouse xenograft model. Cancer Letters 1996, 106: 139-144. PMID: 8827058, DOI: 10.1016/0304-3835(96)04319-4.Peer-Reviewed Original ResearchConceptsHuman colon tumor cellsTumor cellsColon tumor cellsSensitive allele-specific polymerase chain reactionExperimental modelNude mouse xenograft modelK-ras mutationsMouse xenograft modelAllele-specific polymerase chain reactionK-ras geneLymph nodesPrimary implantationSpecific polymerase chain reactionHuman colon carcinoma cellsMicrometastatic depositsPolymerase chain reactionColon carcinoma cellsXenograft modelNude miceGAT mutationMetastatic processCarcinoma cellsBloodChain reactionOccurrence of cells
1992
Stability of K-ras mutations throughout the natural history of human colorectal cancer
Losi L, Benhattar J, Costa J. Stability of K-ras mutations throughout the natural history of human colorectal cancer. European Journal Of Cancer 1992, 28: 1115-1120. PMID: 1627381, DOI: 10.1016/0959-8049(92)90468-h.Peer-Reviewed Original ResearchConceptsK-ras mutationsPrimary tumorRas mutationsNatural historyColorectal cancerTumor markersStable tumor markerHuman colorectal cancerDistant recurrenceRecurrent cancerRecurrent tumorsPolymerase chain reactionColorectal carcinomaCodon 12TumorsArchival tissueCancerChain reactionAllele-specific amplificationPatientsRecurrenceMarkersMutationsCarcinomaDisease