2024
Vascular Endothelial Cells Derived from Transgene-Free Pig Induced Pluripotent Stem Cells for Vascular Tissue Engineering
Batty L, Park J, Qin L, Riaz M, Lin Y, Xu Z, Gao X, Li X, Lopez C, Zhang W, Hoareau M, Fallon M, Huang Y, Luo H, Luo J, Ménoret S, Li P, Jiang Z, Smith P, Sachs D, Tellides G, Anegon I, Pober J, Liu P, Qyang Y. Vascular Endothelial Cells Derived from Transgene-Free Pig Induced Pluripotent Stem Cells for Vascular Tissue Engineering. Acta Biomaterialia 2024 PMID: 39681154, DOI: 10.1016/j.actbio.2024.12.033.Peer-Reviewed Original ResearchInduced pluripotent stem cellsVascular tissue engineeringPig induced pluripotent stem cellsPluripotent stem cellsEndothelial cellsLarge animal modelStem cellsAnimal modelsTissue engineeringInferior vena cava graftHuman induced pluripotent stem cellsEffective differentiation protocolsPreclinical large animal modelExpression of endothelial markersCell-based therapiesExtensive preclinical testingPig endothelial cellsFunctional endothelial cellsIn vivo functional studiesTreatment of cardiovascular diseasesVascular endothelial cellsTissue engineering therapiesTransplant therapeuticsEfficacy of tissueImmunodeficient rats
2020
Ex vivo isolated human vessel perfusion system for the design and assessment of nanomedicines targeted to the endothelium
Lysyy T, Bracaglia LG, Qin L, Albert C, Pober JS, Tellides G, Saltzman WM, Tietjen GT. Ex vivo isolated human vessel perfusion system for the design and assessment of nanomedicines targeted to the endothelium. Bioengineering & Translational Medicine 2020, 5: e10154. PMID: 32440561, PMCID: PMC7237142, DOI: 10.1002/btm2.10154.Peer-Reviewed Original ResearchVessel perfusion systemAnimal modelsPerfusion systemProcess of inflammationVessel segmentsSame human donorEffective clinical translationImmunodeficient miceUmbilical cordRodent modelsHuman donorsEndothelial cellsHuman vesselsBiologic relevanceBiologic replicatesClinical translationSubsequent studiesEfficacyCultured cellsModular chambersCell culturesVivoCellsQuantitative assayVessels