2020
Lassa-VSV chimeric virus targets and destroys human and mouse ovarian cancer by direct oncolytic action and by initiating an anti-tumor response
van den Pol A, Zhang X, Lima E, Pitruzzello M, Albayrak N, Alvero A, Davis J, Mor G. Lassa-VSV chimeric virus targets and destroys human and mouse ovarian cancer by direct oncolytic action and by initiating an anti-tumor response. Virology 2020, 555: 44-55. PMID: 33453650, PMCID: PMC8451984, DOI: 10.1016/j.virol.2020.10.009.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Line, TumorFemaleHumansLassa virusMiceMice, NudeOncolytic VirotherapyOvarian NeoplasmsVesiculovirusConceptsOvarian cancer cellsHuman ovarian cancer cellsLong-term survivalOvarian cancerCancer cellsTreatment of immunocompetent miceKill ovarian cancer cellsMouse ovarian cancer cellsTreatment of ovarian cancerAnti-tumor responsesImmunocompromised nude miceOncolytic actionImmunocompetent miceMetastatic spreadChemotherapeutic paclitaxelFemale cancersPoor survivalNude miceIntraperitoneal injectionCancer growthSurvival extensionVesicular stomatitis virusVSV glycoproteinCancerReduced neurotropismMucin-Like Domain of Ebola Virus Glycoprotein Enhances Selective Oncolytic Actions against Brain Tumors
Zhang X, Zhang T, Davis JN, Marzi A, Marchese AM, Robek MD, van den Pol AN. Mucin-Like Domain of Ebola Virus Glycoprotein Enhances Selective Oncolytic Actions against Brain Tumors. Journal Of Virology 2020, 94: 10.1128/jvi.01967-19. PMID: 32051271, PMCID: PMC7108827, DOI: 10.1128/jvi.01967-19.Peer-Reviewed Original ResearchConceptsChimeric vesicular stomatitis virusVSV-EBOVNormal brain cellsBrain tumorsVesicular stomatitis virusEBOV glycoproteinEbola virus glycoproteinEbola virusBrain cellsNormal immunocompetent miceBrain tumor-bearing miceImmunodeficient SCID miceTumor-bearing miceHuman brain tumorsSCID mouse brainStomatitis virusTranscription-quantitative PCRMost tumor cellsVirus glycoproteinOncolytic actionImmune system evasionImmunocompetent miceWild-type vesicular stomatitis virusSCID miceVirus infection
2015
Lassa-Vesicular Stomatitis Chimeric Virus Safely Destroys Brain Tumors
Wollmann G, Drokhlyansky E, Davis JN, Cepko C, van den Pol AN. Lassa-Vesicular Stomatitis Chimeric Virus Safely Destroys Brain Tumors. Journal Of Virology 2015, 89: 6711-6724. PMID: 25878115, PMCID: PMC4468483, DOI: 10.1128/jvi.00709-15.Peer-Reviewed Original ResearchConceptsLymphocytic choriomeningitis virusBrain tumorsVesicular stomatitis virusChimeric virusesBrain cancerLassa virusEbola virusMarburg virusRabies virusAdverse neurologic consequencesExquisite tumor specificityHigh-grade tumorsPromising oncolytic virusLassa virus glycoprotein precursorAdverse side effectsTumor-bearing miceMetastatic cancer modelsStomatitis virusNovel chimeric virusHigh-grade glioblastomaEbola virus glycoproteinMultiple cancer typesNoninjected tumorNeurologic consequencesOncolytic potential
2013
Vesicular Stomatitis Virus Variants Selectively Infect and Kill Human Melanomas but Not Normal Melanocytes
Wollmann G, Davis JN, Bosenberg MW, van den Pol AN. Vesicular Stomatitis Virus Variants Selectively Infect and Kill Human Melanomas but Not Normal Melanocytes. Journal Of Virology 2013, 87: 6644-6659. PMID: 23552414, PMCID: PMC3676084, DOI: 10.1128/jvi.03311-12.Peer-Reviewed Original ResearchConceptsVesicular stomatitis virusReplication-competent vesicular stomatitis virusMetastatic malignant melanomaRecombinant vesicular stomatitis virusMouse melanoma modelHuman melanoma samplesGene mutation statusVSV-CT9Low viral concentrationsMelanoma typesMalignant melanomaSCID miceViral oncolysisMelanoma xenograftsViral infectionMelanoma modelMutation statusMalignant transformationHuman melanomaInfectionMelanomaVirus variantsComplete protectionMelanoma samplesGene mutations
2009
Viral Mutagenesis as a Means for Generating Novel Proteins
Davis J, van den Pol A. Viral Mutagenesis as a Means for Generating Novel Proteins. Journal Of Virology 2009, 84: 1625-1630. PMID: 19906913, PMCID: PMC2812339, DOI: 10.1128/jvi.01747-09.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell LineCell Line, TumorCricetinaeDNA PrimersGenetic EngineeringMutagenesisVesiculovirusViral Plaque Assay
2005
Mitochondrial DNA depletion analysis by pseudogene ratioing
Swerdlow R, Redpath G, Binder D, Davis J, VandenBerg S. Mitochondrial DNA depletion analysis by pseudogene ratioing. Journal Of Neuroscience Methods 2005, 150: 265-271. PMID: 16118020, DOI: 10.1016/j.jneumeth.2005.06.023.Peer-Reviewed Original ResearchConceptsPlasmid clonesCell linesTreated with ethidium bromideCopies of mtDNAPolymerase chain reactionMtDNA copy numberPCR-based analysisPolymerase chain reaction productsU251 human glioma cell lineMtDNA pseudogenesAmplified mtDNAMitochondrial DNAMtDNAMtDNA depletionGenomic DNACopy numberHuman glioma cell linesPseudogenesGlioma cell linesEthidium bromidePrimersPlasmidClonesDNAChain reaction