Mitochondrial DNA depletion analysis by pseudogene ratioing
Swerdlow R, Redpath G, Binder D, Davis J, VandenBerg S. Mitochondrial DNA depletion analysis by pseudogene ratioing. Journal Of Neuroscience Methods 2005, 150: 265-271. PMID: 16118020, DOI: 10.1016/j.jneumeth.2005.06.023.Peer-Reviewed Original ResearchConceptsPlasmid clonesCell linesTreated with ethidium bromideCopies of mtDNAPolymerase chain reactionMtDNA copy numberPCR-based analysisPolymerase chain reaction productsU251 human glioma cell lineMtDNA pseudogenesAmplified mtDNAMitochondrial DNAMtDNAMtDNA depletionGenomic DNACopy numberHuman glioma cell linesPseudogenesGlioma cell linesEthidium bromidePrimersPlasmidClonesDNAChain reaction