2015
In silico discovery and modeling of non-coding RNA structure in viruses
Moss WN, Steitz JA. In silico discovery and modeling of non-coding RNA structure in viruses. Methods 2015, 91: 48-56. PMID: 26116541, PMCID: PMC4684774, DOI: 10.1016/j.ymeth.2015.06.015.Peer-Reviewed Original Research
2013
RNA families in Epstein–Barr virus
Moss WN, Lee N, Pimienta G, Steitz JA. RNA families in Epstein–Barr virus. RNA Biology 2013, 11: 10-17. PMID: 24441309, PMCID: PMC3929418, DOI: 10.4161/rna.27488.Peer-Reviewed Original ResearchConceptsEpstein-Barr virusFunctional importanceSmall regulatory RNAsNovel Epstein-Barr virusSmall nucleolar RNAsLikely functional importanceInternal ribosomal entry siteRNA-seq studiesHuman γ-herpesvirusEvolutionary conservationNovel ncRNARegulatory RNAsShort intronsRecent bioinformaticsNucleolar RNAsOncogenic typesRNA familiesTumorigenic phenotypeStructured RNAsEBV genomeEBNA1 mRNARepetitive regionsViral latencyHigh abundanceLatency maintenance
2012
AUF1/hnRNP D is a novel protein partner of the EBER1 noncoding RNA of Epstein-Barr virus
Lee N, Pimienta G, Steitz JA. AUF1/hnRNP D is a novel protein partner of the EBER1 noncoding RNA of Epstein-Barr virus. RNA 2012, 18: 2073-2082. PMID: 23012480, PMCID: PMC3479396, DOI: 10.1261/rna.034900.112.Peer-Reviewed Original ResearchMeSH Keywords3' Untranslated RegionsAptamers, NucleotideAU Rich ElementsBinding, CompetitiveCell Line, TumorHerpesvirus 4, HumanHeterogeneous Nuclear Ribonucleoprotein D0Heterogeneous-Nuclear Ribonucleoprotein DHost-Pathogen InteractionsHumansImmunoprecipitationMutagenesis, InsertionalProtein BindingProtein IsoformsRNA StabilityRNA, ViralConceptsAU-rich elementsProtein partnersAUF1/hnRNP DUntranslated regionBacteriophage MS2 coat proteinNovel protein partnersHigh abundanceElectrophoretic mobility shift assaysEpstein-Barr virusMS2 coat proteinStable isotope labelingMobility shift assaysInteracting proteinMolecular functionsHnRNP DAlternative splicingNoncoding RNAsShift assaysCoat proteinIsotope labelingP40 isoformRNA aptamersRNA 1AUF1UV crosslinking
2010
Comprehensive Analysis of Rhesus Lymphocryptovirus MicroRNA Expression
Riley K, Rabinowitz GS, Steitz JA. Comprehensive Analysis of Rhesus Lymphocryptovirus MicroRNA Expression. Journal Of Virology 2010, 84: 5148-5157. PMID: 20219930, PMCID: PMC2863793, DOI: 10.1128/jvi.00110-10.Peer-Reviewed Original Research
2006
Epstein-Barr virus noncoding RNAs are confined to the nucleus, whereas their partner, the human La protein, undergoes nucleocytoplasmic shuttling
Fok V, Friend K, Steitz JA. Epstein-Barr virus noncoding RNAs are confined to the nucleus, whereas their partner, the human La protein, undergoes nucleocytoplasmic shuttling. Journal Of Cell Biology 2006, 173: 319-325. PMID: 16682524, PMCID: PMC2063832, DOI: 10.1083/jcb.200601026.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAnimalsAntibiotics, AntineoplasticAutoantigensCell LineCell Line, TumorCell NucleusDactinomycinFatty Acids, UnsaturatedFemaleHeLa CellsHerpesvirus 4, HumanHumansKaryopherinsMiceNIH 3T3 CellsOocytesProtein BindingRibonucleoproteinsRNA TransportRNA, UntranslatedRNA, ViralXenopus laevisMultiple domains of EBER 1, an Epstein-Barr virus noncoding RNA, recruit human ribosomal protein L22
Fok V, Mitton-Fry RM, Grech A, Steitz JA. Multiple domains of EBER 1, an Epstein-Barr virus noncoding RNA, recruit human ribosomal protein L22. RNA 2006, 12: 872-882. PMID: 16556938, PMCID: PMC1440895, DOI: 10.1261/rna.2339606.Peer-Reviewed Original ResearchMeSH KeywordsBinding SitesCarrier ProteinsCell LineCross-Linking ReagentsElectrophoretic Mobility Shift AssayHerpesvirus 4, HumanHumansIn Vitro TechniquesMaltose-Binding ProteinsNucleic Acid ConformationPlasmidsProtein BindingProtein Structure, TertiaryRecombinant Fusion ProteinsRibosomal ProteinsRNA, UntranslatedRNA, ViralRNA-Binding ProteinsSequence DeletionTranscription, GeneticTransfectionUltraviolet Rays
1994
The Epstein-Barr virus (EBV) small RNA EBER1 binds and relocalizes ribosomal protein L22 in EBV-infected human B lymphocytes.
Toczyski DP, Matera AG, Ward DC, Steitz JA. The Epstein-Barr virus (EBV) small RNA EBER1 binds and relocalizes ribosomal protein L22 in EBV-infected human B lymphocytes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 3463-3467. PMID: 8159770, PMCID: PMC43597, DOI: 10.1073/pnas.91.8.3463.Peer-Reviewed Original ResearchConceptsEpstein-Barr virusHuman B lymphocytesB lymphocytesEBV-positive lymphocytesEBV-positive cellsEBER1 RNAUninfected human cellsCertain patientsEBER1Ribosomal protein L22LymphocytesChromosomal translocationsOncogenic herpesvirusProtein L22Situ hybridizationUninfected cell extractsNucleoplasmic stainingVivoCell transformationHigh levelsHuman cellsCellsPatientsLeukemiaCellular proteins
1993
The Cellular RNA-Binding Protein EAP Recognizes a Conserved Stem-Loop in the Epstein-Barr Virus Small RNA EBER 1
Toczyski D, Steitz J. The Cellular RNA-Binding Protein EAP Recognizes a Conserved Stem-Loop in the Epstein-Barr Virus Small RNA EBER 1. Molecular And Cellular Biology 1993, 13: 703-710. DOI: 10.1128/mcb.13.1.703-710.1993.Peer-Reviewed Original ResearchSmall RNAsCellular RNA-binding proteinsConserved stem loopRNA-binding proteinDetailed mutational analysisStem-loop structureSequence-specific mannerRNase protection experimentsDouble-stranded regionsDeletion mutantsEBER-1Mutational analysisStem loopFusion proteinProtection experimentsViral proteinsRNAProteinEpstein-Barr virus small RNAsEpstein-Barr virusBindingMutantsHerpesvirus papioNucleotidesHairpinThe cellular RNA-binding protein EAP recognizes a conserved stem-loop in the Epstein-Barr virus small RNA EBER 1.
Toczyski DP, Steitz JA. The cellular RNA-binding protein EAP recognizes a conserved stem-loop in the Epstein-Barr virus small RNA EBER 1. Molecular And Cellular Biology 1993, 13: 703-710. PMID: 8380232, PMCID: PMC358948, DOI: 10.1128/mcb.13.1.703.Peer-Reviewed Original ResearchConceptsCellular RNA-binding proteinsRNA-binding proteinDetailed mutational analysisStem-loop structureSequence-specific mannerRNase protection experimentsEpstein‐Barr virus small RNA EBER‐1Double-stranded regionsSmall RNAsDeletion mutantsEBER-1Mutational analysisFusion proteinProtection experimentsViral proteinsProteinRNAEpstein-Barr virusBindingMutantsHerpesvirus papioNucleotidesHairpinAntibodiesCells
1991
EAP, a highly conserved cellular protein associated with Epstein‐Barr virus small RNAs (EBERs).
Toczyski DP, Steitz JA. EAP, a highly conserved cellular protein associated with Epstein‐Barr virus small RNAs (EBERs). The EMBO Journal 1991, 10: 459-466. PMID: 1846807, PMCID: PMC452667, DOI: 10.1002/j.1460-2075.1991.tb07968.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceB-LymphocytesCell LineCell Transformation, ViralCloning, MolecularDNAHerpesvirus 4, HumanHumansMolecular Sequence DataPolymerase Chain ReactionProteinsRibonucleoproteinsRibonucleoproteins, Small NuclearRibosomal ProteinsRNA, Small NuclearRNA, ViralRNA-Binding ProteinsSequence Homology, Nucleic Acid
1986
Localization of Epstein-Barr virus-encoded small RNAs by in situ hybridization.
Howe JG, Steitz JA. Localization of Epstein-Barr virus-encoded small RNAs by in situ hybridization. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 9006-9010. PMID: 3024161, PMCID: PMC387063, DOI: 10.1073/pnas.83.23.9006.Peer-Reviewed Original Research
1981
Striking Similarities Are Exhibited by Two Small Epstein-Barr Virus-Encoded Ribonucleic Acids and the Adenovirus-Associated Ribonucleic Acids VAI and VAII
Rosa M, Gottlieb E, Lerner M, Steitz J. Striking Similarities Are Exhibited by Two Small Epstein-Barr Virus-Encoded Ribonucleic Acids and the Adenovirus-Associated Ribonucleic Acids VAI and VAII. Molecular And Cellular Biology 1981, 1: 785-796. DOI: 10.1128/mcb.1.9.785-796.1981.Peer-Reviewed Original ResearchTwo small RNAs encoded by Epstein-Barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus.
Lerner MR, Andrews NC, Miller G, Steitz JA. Two small RNAs encoded by Epstein-Barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus. Proceedings Of The National Academy Of Sciences Of The United States Of America 1981, 78: 805-809. PMID: 6262773, PMCID: PMC319891, DOI: 10.1073/pnas.78.2.805.Peer-Reviewed Original ResearchConceptsSystemic lupus erythematosusLupus erythematosusEBER-1EBER-2EBV DNAB lymphocytesEpstein-Barr virus genomeEpstein-Barr virusNormal B lymphocytesEcoRI J fragmentT lymphocytesErythematosusLa antigenLymphocytesPrimate cellsHerpesvirus atelesExhibit sequence homologyAntibodiesPotential relationshipVirus genomeCell transformationCellsUninfected mammalian cellsRNAMammalian cells