2023
Baseline skin cytokine profiles determined by RNA in situ hybridization correlate with response to dupilumab in patients with eczematous dermatitis
Singh K, Valido K, Swallow M, Okifo K, Wang A, Cohen J, Damsky W. Baseline skin cytokine profiles determined by RNA in situ hybridization correlate with response to dupilumab in patients with eczematous dermatitis. Journal Of The American Academy Of Dermatology 2023, 88: 1094-1100. PMID: 36780951, DOI: 10.1016/j.jaad.2022.12.052.Peer-Reviewed Original ResearchConceptsEczematous dermatitisHistologic featuresTreatment selectionType 1Type 3 inflammationInflammatory skin diseaseCertain histologic featuresSitu hybridization correlatesClinical responseNonresponder groupCytokine profileCytokine stainingAtopic dermatitisCytokine RNAEczematous disordersSitu hybridizationDupilumabIL13 expressionSkin diseasesType 2PatientsDermatitisNonrespondersMolecular heterogeneityImproved responseIL17A mRNA Staining Distinguishes Palmoplantar Psoriasis from Hyperkeratotic Palmoplantar Eczema in Diagnostic Skin Biopsies
Chen J, Murphy M, Singh K, Wang A, Chow R, Kim R, Cohen J, Ko C, Damsky W. IL17A mRNA Staining Distinguishes Palmoplantar Psoriasis from Hyperkeratotic Palmoplantar Eczema in Diagnostic Skin Biopsies. JID Innovations 2023, 3: 100189. PMID: 37205304, PMCID: PMC10186614, DOI: 10.1016/j.xjidi.2023.100189.Peer-Reviewed Original ResearchMycosis fungoides palmaris et plantarisPalmoplantar psoriasisNonacral sitesPalmoplantar eczemaMRNA expressionAcral skinIL-13 expressionPalmaris et plantarisDiagnostic skin biopsyDermatopathology databaseIL-17AHistopathologic featuresClinical managementBiopsy specimensSkin biopsiesCytokine biomarkersUseful biomarkerEczemaPsoriasisDiagnostic clarityMRNA stainingDermatosesBiomarkersSitu hybridizationSkin
2021
Cytokine RNA In Situ Hybridization Permits Individualized Molecular Phenotyping in Biopsies of Psoriasis and Atopic Dermatitis
Wang A, Fogel AL, Murphy MJ, Panse G, McGeary MK, McNiff JM, Bosenberg M, Vesely MD, Cohen JM, Ko CJ, King BA, Damsky W. Cytokine RNA In Situ Hybridization Permits Individualized Molecular Phenotyping in Biopsies of Psoriasis and Atopic Dermatitis. JID Innovations 2021, 1: 100021. PMID: 34909719, PMCID: PMC8659380, DOI: 10.1016/j.xjidi.2021.100021.Peer-Reviewed Original ResearchAtopic dermatitisAtopic dermatitis casesInflammatory skin diseaseInflammatory skin disorderSubset of casesSitu hybridizationCytokine RNARoutine biopsyCytokine expressionPredominant cytokinePsoriasis casesCytokine targetsTreatment selectionDermatitis casesSkin disordersPsoriasis biomarkersSkin diseasesIndividual cytokinesIndividualized evaluationPsoriasisBiopsyBulk RNA sequencingCytokinesMolecular phenotypingPatients