2014
Chemokine-coupled β2 integrin–induced macrophage Rac2–Myosin IIA interaction regulates VEGF-A mRNA stability and arteriogenesis
Morrison AR, Yarovinsky TO, Young BD, Moraes F, Ross TD, Ceneri N, Zhang J, Zhuang ZW, Sinusas AJ, Pardi R, Schwartz MA, Simons M, Bender JR. Chemokine-coupled β2 integrin–induced macrophage Rac2–Myosin IIA interaction regulates VEGF-A mRNA stability and arteriogenesis. Journal Of Experimental Medicine 2014, 211: 1957-1968. PMID: 25180062, PMCID: PMC4172219, DOI: 10.1084/jem.20132130.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArteriesCD18 AntigensDNA PrimersFlow CytometryHumansMiceMice, Inbred C57BLMonocytesNeovascularization, PhysiologicNonmuscle Myosin Type IIARac GTP-Binding ProteinsReal-Time Polymerase Chain ReactionReceptors, CCR2RNA StabilityVascular Endothelial Growth Factor AX-Ray MicrotomographyConceptsMyosin IIASignal transduction eventsHuR translocationRapid nuclearTransduction eventsProteomic analysisProtein HuR.Induction of arteriogenesisMRNA stabilityMRNA stabilizationNovel roleCytosolic translocationMyosin-9ICAM-1 adhesionReceptor engagementDevelopmental vasculogenesisCellular effectorsMolecular triggersTranslocationHeavy chainGrowth factorMyeloid cellsVascular endothelial growth factorKey molecular triggerCCL2 stimulation
2013
Transmembrane protein ESDN promotes endothelial VEGF signaling and regulates angiogenesis
Nie L, Guo X, Esmailzadeh L, Zhang J, Asadi A, Collinge M, Li X, Kim JD, Woolls M, Jin SW, Dubrac A, Eichmann A, Simons M, Bender JR, Sadeghi MM. Transmembrane protein ESDN promotes endothelial VEGF signaling and regulates angiogenesis. Journal Of Clinical Investigation 2013, 123: 5082-5097. PMID: 24177422, PMCID: PMC3859420, DOI: 10.1172/jci67752.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDBlood VesselsCadherinsCells, CulturedEar, ExternalEndothelium, VascularHindlimbHuman Umbilical Vein Endothelial CellsHumansIschemiaMembrane ProteinsMiceMice, Inbred C57BLMice, KnockoutNeovascularization, PhysiologicNeuropilinsProtein Tyrosine Phosphatase, Non-Receptor Type 1Protein Tyrosine Phosphatase, Non-Receptor Type 2Retinal VesselsRNA InterferenceRNA, Small InterferingVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2ZebrafishZebrafish ProteinsConceptsSmooth muscle cell-derived neuropilin-like proteinAberrant blood vessel formationNormal vascular developmentProtein tyrosineTC-PTPTransmembrane proteinTherapeutic targetBlood vessel formationVEGF responseNegative regulatorDevelopmental angiogenesisVEGFR-2Vascular developmentAttractive therapeutic targetESDNAngiogenesis regulationVE-cadherinVessel formationEC proliferationComplex formationRegulatorProteinNeuropilin expressionVEGF receptorsEndothelial VEGF
2012
Molecular Imaging of Vascular Endothelial Growth Factor Receptors in Graft Arteriosclerosis
Zhang J, Razavian M, Tavakoli S, Nie L, Tellides G, Backer JM, Backer MV, Bender JR, Sadeghi MM. Molecular Imaging of Vascular Endothelial Growth Factor Receptors in Graft Arteriosclerosis. Arteriosclerosis Thrombosis And Vascular Biology 2012, 32: 1849-1855. PMID: 22723442, PMCID: PMC3401339, DOI: 10.1161/atvbaha.112.252510.Peer-Reviewed Original ResearchConceptsGraft arteriosclerosisArtery graftVascular remodelingAllogeneic human peripheral blood mononuclear cellsHuman coronary artery segmentsHuman peripheral blood mononuclear cellsPeripheral blood mononuclear cellsSevere combined immunodeficiency miceVEGF receptorsVascular endothelial growth factor receptorLate organ failureHuman coronary artery graftsSolid organ transplantationCoronary artery graftsEndothelial growth factor receptorBlood mononuclear cellsCoronary artery segmentsCombined immunodeficiency miceReceptor 2 expressionVEGF receptor-2 expressionMolecular imagingSignificant neointima formationVascular endothelial growth factor signalingVEGF receptor 1Growth factor receptorMacrophage β2 Integrin–Mediated, HuR-Dependent Stabilization of Angiogenic Factor–Encoding mRNAs in Inflammatory Angiogenesis
Zhang J, Modi Y, Yarovinsky T, Yu J, Collinge M, Kyriakides T, Zhu Y, Sessa WC, Pardi R, Bender JR. Macrophage β2 Integrin–Mediated, HuR-Dependent Stabilization of Angiogenic Factor–Encoding mRNAs in Inflammatory Angiogenesis. American Journal Of Pathology 2012, 180: 1751-1760. PMID: 22322302, PMCID: PMC3349897, DOI: 10.1016/j.ajpath.2011.12.025.Peer-Reviewed Original ResearchMeSH KeywordsAngiogenesis Inducing AgentsAnimalsCD18 AntigensCell AdhesionCells, CulturedDisease Models, AnimalELAV ProteinsGene Expression RegulationGene Knockout TechniquesHindlimbInflammationIschemiaMacrophagesMiceMice, KnockoutMuscle, SkeletalNeovascularization, PathologicReal-Time Polymerase Chain ReactionRNA, MessengerConceptsKnockout miceAngiogenic factorsT cell cytokine productionIntercellular adhesion molecule-1Blood flow recoveryFemoral artery ligationLittermate wild-type controlsVascular endothelial growth factorBone marrow-derived macrophagesMatrix metalloproteinase-9Adhesion molecule-1Endothelial growth factorMarrow-derived macrophagesSoluble factor productionWild-type controlsArtery ligationLigand intercellular adhesion molecule-1Cytokine productionInflammatory angiogenesisMetalloproteinase-9Tissue ischemiaInflammatory stimuliMolecule-1Macrophage productionNeovascular response
2010
T Cell LFA-1 Engagement Induces HuR-Dependent Cytokine mRNA Stabilization through a Vav-1, Rac1/2, p38MAPK and MKK3 Signaling Cascade
Ramgolam VS, DeGregorio SD, Rao GK, Collinge M, Subaran SS, Markovic-Plese S, Pardi R, Bender JR. T Cell LFA-1 Engagement Induces HuR-Dependent Cytokine mRNA Stabilization through a Vav-1, Rac1/2, p38MAPK and MKK3 Signaling Cascade. PLOS ONE 2010, 5: e14450. PMID: 21206905, PMCID: PMC3012057, DOI: 10.1371/journal.pone.0014450.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, SurfaceCytokinesELAV ProteinsELAV-Like Protein 1GTP PhosphohydrolasesHumansIntegrinsInterferon-gammaLymphocyte Function-Associated Antigen-1MAP Kinase Kinase 3MiceMice, Inbred C57BLNeuropeptidesP38 Mitogen-Activated Protein KinasesProto-Oncogene Proteins c-vavRac GTP-Binding ProteinsRac1 GTP-Binding ProteinRNA-Binding ProteinsSignal TransductionT-LymphocytesTumor Necrosis Factor-alpha
2008
Rac, PAK, and eNOS ACTion
Rao GK, Bender JR. Rac, PAK, and eNOS ACTion. Circulation Research 2008, 103: 328-330. PMID: 18703782, DOI: 10.1161/circresaha.108.182238.Peer-Reviewed Original ResearchTargeted inactivation of the COP9 signalosome impairs multiple stagesof T cell development
Panattoni M, Sanvito F, Basso V, Doglioni C, Casorati G, Montini E, Bender JR, Mondino A, Pardi R. Targeted inactivation of the COP9 signalosome impairs multiple stagesof T cell development. Journal Of Experimental Medicine 2008, 205: 465-477. PMID: 18268034, PMCID: PMC2271025, DOI: 10.1084/jem.20070725.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBcl-X ProteinCell CycleCell LineCell ProliferationCOP9 Signalosome ComplexCyclin-Dependent Kinase Inhibitor p16DNA RepairFemaleHomeodomain ProteinsIntracellular Signaling Peptides and ProteinsMiceMice, Inbred C57BLMice, KnockoutMultiprotein ComplexesNF-kappa BPeptide HydrolasesProto-Oncogene Proteins c-bcl-2Receptors, Antigen, T-CellRNA, MessengerT-LymphocytesTumor Suppressor Protein p53Ubiquitin-Protein LigasesConceptsCOP9 signalosomeCSN5/Jab1Genetic programBcl-2 family membersGenetic complementation analysisBcl-xL/BclS-phase progressionDistinct developmental stagesCell cycle progressionT cell developmentComplementation analysisLower organismsCatalytic subunitPositive selectionTranscription factorsDNA repairCycle progressionCell developmentThymocyte survivalDevelopmental stagesNF-kappaB pathwayTransgenic backgroundPhase progressionRapid turnoverEffector molecules
2007
Variant estrogen receptor–c-Src molecular interdependence and c-Src structural requirements for endothelial NO synthase activation
Li L, Hisamoto K, Kim KH, Haynes MP, Bauer PM, Sanjay A, Collinge M, Baron R, Sessa WC, Bender JR. Variant estrogen receptor–c-Src molecular interdependence and c-Src structural requirements for endothelial NO synthase activation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2007, 104: 16468-16473. PMID: 17921256, PMCID: PMC2034248, DOI: 10.1073/pnas.0704315104.Peer-Reviewed Original ResearchConceptsC-SrcC-Src kinase activityTyrosine kinase c-SrcRapid signal transductionKinase c-SrcC-Src functionC-Src kinaseEndothelial NO synthase activationENOS activationMembrane recruitmentSignal transductionComplex assemblyPlasma membraneKinase activityOestrogen receptor-alpha variantsVenous endothelial cellsER46Pathway activationHormonal stimuliCritical roleArterial responseNO synthase activationSynthase activationStructural requirementsEndothelial cellsESDN Is a Marker of Vascular Remodeling and Regulator of Cell Proliferation in Graft Arteriosclerosis
Sadeghi MM, Esmailzadeh L, Zhang J, Guo X, Asadi A, Krassilnikova S, Fassaei HR, Luo G, Al‐Lamki R, Takahashi T, Tellides G, Bender JR, Rodriguez ER. ESDN Is a Marker of Vascular Remodeling and Regulator of Cell Proliferation in Graft Arteriosclerosis. American Journal Of Transplantation 2007, 7: 2098-2105. PMID: 17697260, DOI: 10.1111/j.1600-6143.2007.01919.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersCell ProliferationCells, CulturedCoronary Artery DiseaseCoronary VesselsDisease Models, AnimalHumansImmunohistochemistryMembrane ProteinsMiceMice, SCIDMuscle, Smooth, VascularReverse Transcriptase Polymerase Chain ReactionRNATissue TransplantationTransplantation, HomologousUp-RegulationConceptsGraft arteriosclerosisVascular remodelingCell proliferationSevere combined immunodeficient (SCID) miceInjury-induced vascular remodelingCombined Immunodeficient MiceHuman coronary arteriesVascular smooth muscle cell culturesVascular cell proliferationSmooth muscle cell culturesCell reconstitutionCoronary arteryImmunodeficient miceVSMC proliferationNormal arteriesArteryHuman coronaryMuscle cell culturesDiagnostic targetsHuman arteriesHigh levelsRemodelingSmooth muscle cell-derived neuropilin-like proteinArteriosclerosisProliferation
2005
αvβ3‐Targeted detection of arteriopathy in transplanted human coronary arteries: an autoradiographic study
Zhang J, Krassilnikova S, Gharaei AA, Fassaei HR, Esmailzadeh L, Asadi A, Edwards DS, Harris TD, Azure M, Tellides G, Sinusas AJ, Zaret BL, Bender JR, Sadeghi MM. αvβ3‐Targeted detection of arteriopathy in transplanted human coronary arteries: an autoradiographic study. The FASEB Journal 2005, 19: 1857-1859. PMID: 16150802, DOI: 10.1096/fj.05-4130fje.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaAutoradiographyCarotid Artery DiseasesCell MovementCell ProliferationCell TransplantationCells, CulturedChimeraCoronary VesselsDensitometryEndothelium, VascularHeart TransplantationHeterocyclic Compounds, 1-RingHumansImmunohistochemistryIntegrin alphaVbeta3Ki-67 AntigenLeukocytes, MononuclearMiceMice, SCIDMicroscopy, FluorescenceOrganometallic CompoundsRecombinant Fusion ProteinsTime FactorsTissue TransplantationUp-RegulationVascular DiseasesConceptsPeripheral blood mononuclear cellsGraft arteriopathyHuman coronary arteriesCoronary arteryAllogeneic human peripheral blood mononuclear cellsHuman/mouse chimeric modelAlphavbeta3 expressionHuman peripheral blood mononuclear cellsProliferative processesSevere combined immunodeficiency miceLate graft failureBlood mononuclear cellsCombined immunodeficiency miceAlphavbeta3 integrinSpecificity of uptakeCardiac transplantationConcentric narrowingGraft failureMononuclear cellsImmunodeficiency miceVascular remodelingNeointima formationNative aortaChimeric modelAutoradiographic study
2004
Detection of Injury-Induced Vascular Remodeling by Targeting Activated αvβ3 Integrin In Vivo
Sadeghi MM, Krassilnikova S, Zhang J, Gharaei AA, Fassaei HR, Esmailzadeh L, Kooshkabadi A, Edwards S, Yalamanchili P, Harris TD, Sinusas AJ, Zaret BL, Bender JR. Detection of Injury-Induced Vascular Remodeling by Targeting Activated αvβ3 Integrin In Vivo. Circulation 2004, 110: 84-90. PMID: 15210600, DOI: 10.1161/01.cir.0000133319.84326.70.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApolipoproteins EArterial Occlusive DiseasesCarbocyaninesCarotid StenosisCell ProliferationCells, CulturedEndothelium, VascularFemaleHeterocyclic Compounds, 1-RingHumansIntegrin alphaVbeta3MiceMice, KnockoutOrganometallic CompoundsRadioactive TracersSulfonamidesTomography, Emission-Computed, Single-PhotonConceptsCounts/pixelCarotid areaCarotid artery wire injuryEndothelial cellsIntegrin expressionInjury-induced remodelingCell proliferationDetection of injuryVascular cell proliferationCultured endothelial cellsCarotid injuryBeta3 integrin expressionWire injuryVascular proliferationRP748Vascular remodelingApolipoprotein EKi67 stainingRenal clearanceEC bindingProliferation indexSpecific radiotracersInjuryProliferative processesWeeksVascular cell adhesion molecule-1-targeted detection of endothelial activation in human microvasculature
Sadeghi MM, Schechner JS, Krassilnikova S, Gharaei AA, Zhang J, Kirkiles-Smith N, Sinusas AJ, Zaret BL, Bender JR. Vascular cell adhesion molecule-1-targeted detection of endothelial activation in human microvasculature. Transplantation Proceedings 2004, 36: 1585-1591. PMID: 15251390, DOI: 10.1016/j.transproceed.2004.05.060.Peer-Reviewed Original ResearchConceptsChimeric human/mouse modelHuman/mouse modelHuman skin graftsEndothelial activationSkin graftsEndothelial cellsMouse modelIsotype-matched control antibodySCID/beige miceTumor necrosis factorEndothelial adhesion moleculesMurine VCAM-1Murine red blood cellsRed blood cellsHuman endothelial cellsControl antibodyInflammatory responseNecrosis factorBeige miceTc-99mVCAM-1Tissue vascularityMonoclonal antibodiesBlood cellsAdhesion molecules
2002
Src Kinase Mediates Phosphatidylinositol 3-Kinase/Akt-dependent Rapid Endothelial Nitric-oxide Synthase Activation by Estrogen*
Haynes MP, Li L, Sinha D, Russell KS, Hisamoto K, Baron R, Collinge M, Sessa WC, Bender JR. Src Kinase Mediates Phosphatidylinositol 3-Kinase/Akt-dependent Rapid Endothelial Nitric-oxide Synthase Activation by Estrogen*. Journal Of Biological Chemistry 2002, 278: 2118-2123. PMID: 12431978, DOI: 10.1074/jbc.m210828200.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsBlotting, WesternCell LineCells, CulturedElectrophoresis, Polyacrylamide GelEndoplasmic ReticulumEndothelium, VascularEnzyme ActivationEnzyme InhibitorsEstrogensHumansMiceMutationNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IINitric Oxide Synthase Type IIIPhosphatidylinositol 3-KinasesPhosphorylationPrecipitin TestsProtein BindingProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktReceptors, EstrogenSignal TransductionSrc-Family KinasesTime FactorsTransfectionTyrosineConceptsC-SrcPI3-kinaseAkt phosphorylationSrc kinaseUpstream regulatorKinase-dead c-SrcC-Src associationActive c-SrcC-Src phosphorylationMurine embryonic fibroblastsBasal Akt phosphorylationC-Src expressionCritical upstream regulatorEndothelial nitric oxide synthaseSrc familyActive AktEmbryonic fibroblastsComplex formation resultsEndothelial cellsHuman endothelial cellsAkt activationPhosphorylationKinaseAktPhosphatidylinositol
1999
Neuregulin activation of ErbB receptors in vascular endothelium leads to angiogenesis
Russell K, Stern D, Polverini P, Bender J. Neuregulin activation of ErbB receptors in vascular endothelium leads to angiogenesis. American Journal Of Physiology 1999, 277: h2205-h2211. PMID: 10600838, DOI: 10.1152/ajpheart.1999.277.6.h2205.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedCorneaEndothelial Growth FactorsEndothelium, VascularErbB ReceptorsHumansLymphokinesMiceNeovascularization, PhysiologicNeuregulinsRatsReceptor, ErbB-2Receptor, ErbB-3Receptor, ErbB-4ThrombinUmbilical VeinsVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsConceptsHuman umbilical vein endothelial cellsVascular endotheliumErbB receptorsReceptor tyrosine phosphorylationStimulation of HUVECsRapid calcium fluxReceptor family membersEndothelial cell growth factorTransmembrane tyrosine kinase receptorVascular endothelial cell growth factorEndothelial cell signalingReceptor-ligand interactionsTyrosine kinase receptorsEpidermal growth factor receptorVascular endothelial growthCell growth factorUmbilical vein endothelial cellsCell signalingGrowth factor receptorTyrosine phosphorylationVivo angiogenic responseExpression patternsGrowth regulationVein endothelial cellsIntracellular signaling
1998
Lymphotoxin alpha3 induces chemokines and adhesion molecules: insight into the role of LT alpha in inflammation and lymphoid organ development.
Cuff C, Schwartz J, Bergman C, Russell K, Bender J, Ruddle N. Lymphotoxin alpha3 induces chemokines and adhesion molecules: insight into the role of LT alpha in inflammation and lymphoid organ development. The Journal Of Immunology 1998, 161: 6853-60. PMID: 9862717, DOI: 10.4049/jimmunol.161.12.6853.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Adhesion MoleculesChemokine CCL2Chemokine CCL4Chemokine CCL5Chemokine CXCL10Chemokine CXCL2ChemokinesChemokines, CXCCytotoxicity, ImmunologicEmbryonic and Fetal DevelopmentE-SelectinGene Expression RegulationHumansImmunoglobulinsInflammationIntercellular Adhesion Molecule-1Lymphoid TissueLymphotoxin-alphaMacrophage Inflammatory ProteinsMiceMonokinesMucoproteinsRecombinant Fusion ProteinsSpecies SpecificityStimulation, ChemicalTumor Cells, CulturedVascular Cell Adhesion Molecule-1ConceptsLymphoid organ developmentMAdCAM-1LT-alphaLT alpha3Adhesion moleculesTreatment periodIFN-inducible protein-10Biologic activityInduction of chemokinesChemotactic protein-1Peripheral node addressinCellular adhesion moleculesMucosal addressin MAdCAM-1Unique biologic activityInflammatory mediatorsChemokine expressionProinflammatory effectsLymphoid tissueMTNF-alphaMaximal killingChemokine RANTESE-selectinKnockout miceProtein 10Alpha preparations
1996
Overlapping recognition of xenogeneic carbohydrate ligands by human natural killer lymphocytes and natural antibodies.
Inverardi L, Clissi B, Stolzer A, Bender J, Pardi R. Overlapping recognition of xenogeneic carbohydrate ligands by human natural killer lymphocytes and natural antibodies. Transplantation Proceedings 1996, 28: 552. PMID: 8623263.Peer-Reviewed Original Research
1994
Epican, a heparan/chondroitin sulfate proteoglycan form of CD44, mediates cell-cell adhesion
Milstone L, Hough-Monroe L, Kugelman L, Bender J, Haggerty J. Epican, a heparan/chondroitin sulfate proteoglycan form of CD44, mediates cell-cell adhesion. Journal Of Cell Science 1994, 107: 3183-3190. PMID: 7699015, DOI: 10.1242/jcs.107.11.3183.Peer-Reviewed Original Research
1993
Heterogeneity of dermal microvascular endothelial cell antigen expression and cytokine responsiveness in situ and in cell culture.
Petzelbauer P, Bender JR, Wilson J, Pober JS. Heterogeneity of dermal microvascular endothelial cell antigen expression and cytokine responsiveness in situ and in cell culture. The Journal Of Immunology 1993, 151: 5062-72. PMID: 7691964, DOI: 10.4049/jimmunol.151.9.5062.Peer-Reviewed Original ResearchConceptsVCAM-1 expressionDermal microvascular endothelial cellsSuperficial vascular plexusAdhesion molecule-1 expressionDeep vascular plexusMolecule-1 expressionIL-1IL-4Molecule expressionVascular bedAntigen expressionCD36 expressionEndothelial leukocyte adhesion molecule-1 expressionELAM-1Normal skinEndothelial cell antigen expressionCytokine responsivenessVascular cell adhesion molecule-1 expressionVascular plexusCapillary loopsCell adhesion molecule-1 expressionParticular vascular bedEndothelial leukocyte adhesion moleculeAdhesion molecule expressionSites of inflammation