2000
Isolation of lactate dehydrogenase-elevating viruses from wild house mice and their biological and molecular characterization
Li K, Schuler T, Chen Z, Glass G, Childs J, Plagemann P. Isolation of lactate dehydrogenase-elevating viruses from wild house mice and their biological and molecular characterization. Virus Research 2000, 67: 153-162. PMID: 10867194, DOI: 10.1016/s0168-1702(00)00142-8.Peer-Reviewed Original Research
1999
Population Dynamics of a Naturally Occurring Heterogeneous Mixture of Borrelia burgdorferiClones
Hofmeister E, Glass G, Childs J, Persing D. Population Dynamics of a Naturally Occurring Heterogeneous Mixture of Borrelia burgdorferiClones. Infection And Immunity 1999, 67: 5709-5716. PMID: 10531219, PMCID: PMC96945, DOI: 10.1128/iai.67.11.5709-5716.1999.Peer-Reviewed Original ResearchConceptsC3H/HeJ miceC.B-17 miceLarval Ixodes scapularis ticksMajority of miceB. burgdorferiInfected nymphal ticksIxodes scapularis ticksBurgdorferi antibodiesSecondary challengeHeJ micePrimary isolatesSequential isolatesC3H miceSequential infectionMiceMonoclonal antibodiesBorrelia burgdorferiC expressionScapularis ticksClonal dominanceUnique isolatesMacrorestriction analysisOspC proteinsClonal groupsEnzootic siteDusky-Footed Wood Rats (Neotoma fuscipes) as Reservoirs of Granulocytic Ehrlichiae (Rickettsiales: Ehrlichieae) in Northern California
Nicholson W, Castro M, Kramer V, Sumner J, Childs J. Dusky-Footed Wood Rats (Neotoma fuscipes) as Reservoirs of Granulocytic Ehrlichiae (Rickettsiales: Ehrlichieae) in Northern California. Journal Of Clinical Microbiology 1999, 37: 3323-3327. PMID: 10488199, PMCID: PMC85556, DOI: 10.1128/jcm.37.10.3323-3327.1999.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCaliforniaDisease ReservoirsDNA, ViralEhrlichiaHumansIxodesMaleMolecular Sequence DataPeromyscusPolymerase Chain ReactionRatsSigmodontinaeConceptsBlood samplesBlood specimensDusky-footed wood ratsConcurrent high prevalenceSeronegative blood samplesHuman granulocytic ehrlichiosisGroESL heat shock operonGranulocytic ehrlichiaePCR-positive animalsPCR positivityHigh prevalencePCR-positive ticksSeropositive specimensRat blood samplesRatsGranulocytic ehrlichiosisEhrlichial DNAPacificus adultsEhrlichia equiEhrlichial agentsMicePCR assaysAdultsWood ratsRodentsMolecular Cloning and Characterization of the Ehrlichia chaffeensis Variable-Length PCR Target: an Antigen-Expressing Gene That Exhibits Interstrain Variation
Sumner J, Childs J, Paddock C. Molecular Cloning and Characterization of the Ehrlichia chaffeensis Variable-Length PCR Target: an Antigen-Expressing Gene That Exhibits Interstrain Variation. Journal Of Clinical Microbiology 1999, 37: 1447-1453. PMID: 10203503, PMCID: PMC84798, DOI: 10.1128/jcm.37.5.1447-1453.1999.Peer-Reviewed Original ResearchConceptsOpen reading frameAmino acid sequenceApparent molecular massE. chaffeensisGenomic libraryMolecular cloningReading frameAcid sequenceDifferentiation of isolatesPCR targetsClose homologyMolecular massNucleotide sequencingImmunoreactive proteinDifferent isolatesDNA samplesInterstrain variationGenesRepeat unitsMolecular databasesCell culturesAmblyomma americanum ticksSequencePCR detectionChaffeensis
1997
Isolation and characterization of Ehrlichia chaffeensis strains from patients with fatal ehrlichiosis
Paddock C, Sumner J, Shore G, Bartley D, Elie R, McQuade J, Martin C, Goldsmith C, Childs J. Isolation and characterization of Ehrlichia chaffeensis strains from patients with fatal ehrlichiosis. Journal Of Clinical Microbiology 1997, 35: 2496-2502. PMID: 9316896, PMCID: PMC229999, DOI: 10.1128/jcm.35.10.2496-2502.1997.Peer-Reviewed Original ResearchConceptsE. chaffeensisDH82 cellsEhrlichia chaffeensisArkansas isolateGroESL heat shock operonFatal ehrlichiosisMechanisms of diseasePulmonary insufficiencyClinical presentationMarked thrombocytopeniaSevere diseaseEhrlichial infectionCharacteristic manifestationsPatientsDays postinoculationMolecular evaluationChaffeensisPrimary isolationRRNA gene sequencesDiseaseIsolatesSpecific associationNew isolatesGene sequencesProtein geneAn indirect immunofluorescence assay using a cell culture-derived antigen for detection of antibodies to the agent of human granulocytic ehrlichiosis
Nicholson W, Comer J, Sumner J, Gingrich-Baker C, Coughlin R, Magnarelli L, Olson J, Childs J. An indirect immunofluorescence assay using a cell culture-derived antigen for detection of antibodies to the agent of human granulocytic ehrlichiosis. Journal Of Clinical Microbiology 1997, 35: 1510-1516. PMID: 9163471, PMCID: PMC229776, DOI: 10.1128/jcm.35.6.1510-1516.1997.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisCell culture-derived antigenHuman antibodiesGranulocytic ehrlichiosisIndirect immunofluorescenceHGE agentAnimal-derived antigensDetection of antibodiesTick-derived isolatesPromyelocytic leukemia cell lineAntigen presentationHuman promyelocytic leukemia cell lineHealthy individualsLeukemia cell linesHGE patientsSera reactiveEhrlichia phagocytophilaHigh titersAntigenImmunofluorescence methodE. equiEhrlichia equiRoutine testingAntibodiesSpecific antibodies
1995
Ehrlichiosis in a Golf-Oriented Retirement Community
Standaert S, Dawson J, Schaffner W, Childs J, Biggie K, Singleton J, Gerhardt R, Knight M, Hutcheson R. Ehrlichiosis in a Golf-Oriented Retirement Community. New England Journal Of Medicine 1995, 333: 420-425. PMID: 7616991, DOI: 10.1056/nejm199508173330704.Peer-Reviewed Original ResearchConceptsE. chaffeensis infectionCase-control studySerologic testingCommunity AMedical history reviewLone star tickRisk of infectionCommunity BAnnual incidenceSerologic evidencePolymerase chain reactionTick biteRetirement communityInfectionEhrlichiosisEndemic diseaseChain reactionEhrlichia chaffeensisHigh rateSurvey participantsPercentTicksInsect repellentOutbreakRecreational activities
1994
Susceptibility of white-tailed deer (Odocoileus virginianus) to infection with Ehrlichia chaffeensis, the etiologic agent of human ehrlichiosis
Dawson J, Stallknecht D, Howerth E, Warner C, Biggie K, Davidson W, Lockhart J, Nettles V, Olson J, Childs J. Susceptibility of white-tailed deer (Odocoileus virginianus) to infection with Ehrlichia chaffeensis, the etiologic agent of human ehrlichiosis. Journal Of Clinical Microbiology 1994, 32: 2725-2728. PMID: 7852563, PMCID: PMC264150, DOI: 10.1128/jcm.32.11.2725-2728.1994.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceDeerDisease ReservoirsEhrlichia chaffeensisEhrlichiosisFemaleHumansMaleMolecular Sequence DataPolymerase Chain ReactionConceptsHuman ehrlichiosisE. canisE. chaffeensisEtiologic agentAbsence of seroconversionEhrlichia chaffeensisMacrophage-type cellsE. chaffeensis infectionLymph nodesPeripheral bloodClinical diseaseDay 10InfectionEhrlichia canisEhrlichiosisAntibodiesWhite-tailed deerChaffeensisNegative controlCanisReservoir hostsCompetent reservoirsWeeksTick vectorSeroconversionSerologic and Genetic Identification of Peromyscus maniculatus as the Primary Rodent Reservoir for a New Hantavirus in the Southwestern United States
Childs J, Ksiazek T, Spiropoulou C, Krebs J, Morzunov S, Maupin G, Gage K, Rollin P, Sarisky J, Enscore R, Frey J, Peters C, Nichol S. Serologic and Genetic Identification of Peromyscus maniculatus as the Primary Rodent Reservoir for a New Hantavirus in the Southwestern United States. The Journal Of Infectious Diseases 1994, 169: 1271-1280. PMID: 8195603, DOI: 10.1093/infdis/169.6.1271.Peer-Reviewed Original Research
1993
Genetic Identification of a Hantavirus Associated with an Outbreak of Acute Respiratory Illness
Nichol S, Spiropoulou C, Morzunov S, Rollin P, Ksiazek T, Feldmann H, Sanchez A, Childs J, Zaki S, Peters C. Genetic Identification of a Hantavirus Associated with an Outbreak of Acute Respiratory Illness. Science 1993, 262: 914-917. PMID: 8235615, DOI: 10.1126/science.8235615.Peer-Reviewed Original Research
1992
Comparison of polymerase chain reaction and culture for detection of Borrelia burgdorferi in naturally infected Peromyscus leucopus and experimentally infected C.B-17 scid/scid mice
Hofmeister E, Markham R, Childs J, Arthur R. Comparison of polymerase chain reaction and culture for detection of Borrelia burgdorferi in naturally infected Peromyscus leucopus and experimentally infected C.B-17 scid/scid mice. Journal Of Clinical Microbiology 1992, 30: 2625-2631. PMID: 1400962, PMCID: PMC270489, DOI: 10.1128/jcm.30.10.2625-2631.1992.Peer-Reviewed Original Research