1995
Vesicle targeting to the apical domain regulates bile excretory function in isolated rat hepatocyte couplets
Boyer J, Soroka C. Vesicle targeting to the apical domain regulates bile excretory function in isolated rat hepatocyte couplets. Gastroenterology 1995, 109: 1600-1611. PMID: 7557144, DOI: 10.1016/0016-5085(95)90649-5.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBile Acids and SaltsBile CanaliculiBucladesineCa(2+) Mg(2+)-ATPaseCells, CulturedLiverMicroscopy, ConfocalMicrotubulesNocodazoleRatsConceptsInsertion of vesiclesTranslocation of vesiclesMembrane transport activityVesicle trafficApical domainIsolated rat hepatocyte coupletsAcid transportersTransport activityNocodazoleRat hepatocyte coupletsVesiclesIndependent mechanismsEpithelial cellsBile acid transporterSite of residenceHepatocyte coupletsTransportersCanalicular membraneRat hepatocytesImmunocytochemical localizationCyclic monophosphateAdenosine triphosphataseBile excretory function
1990
Cryptic na+, k+‐atpase activity in rat liver canalicular plasma membranes: Evidence for its basolateral origin
Sellinger M, Barrett C, Malle P, Gordon E, Boyer J. Cryptic na+, k+‐atpase activity in rat liver canalicular plasma membranes: Evidence for its basolateral origin. Hepatology 1990, 11: 223-229. PMID: 2155168, DOI: 10.1002/hep.1840110211.Peer-Reviewed Original ResearchConceptsFree-flow electrophoresisMembrane-fluidizing agentSurface chargeBasolateral enzymeBasolateral marker enzymeSmall contaminantsRat liver canalicular plasma membranesSmall amountRoutine assayAnodeSucrose density centrifugationMembraneElectrophoresisCanalicular liver plasma membranesEnzymeMembrane preparationsContaminantsPreparationChargeCanalicular markerPrecipitationLiver plasma membranesFractionActivity
1984
Isolated rat hepatocyte couplets: a primary secretory unit for electrophysiologic studies of bile secretory function.
Graf J, Gautam A, Boyer J. Isolated rat hepatocyte couplets: a primary secretory unit for electrophysiologic studies of bile secretory function. Proceedings Of The National Academy Of Sciences Of The United States Of America 1984, 81: 6516-6520. PMID: 6149546, PMCID: PMC391955, DOI: 10.1073/pnas.81.20.6516.Peer-Reviewed Original ResearchConceptsBile secretory functionElectrophysiologic studySecretory functionHepatocyte coupletsOrganic anion fluoresceinSteady-state intracellularCanalicular lumenCanalicular vacuoleInput resistanceRat liverCollagenase perfusionIntact liverLiverSecretionParacellular barrierBile canalicular lumenSecretory unitsLumenCellsJunctional pathwayElectrical driving forcePerfusion
1983
Biochemical Separation of Na+, K+‐ATPase from a “Purified” Light Density, “Canalicular”‐Enriched Plasma Membrane Fraction from Rat Liver
Boyer J, Allen R, Ng O. Biochemical Separation of Na+, K+‐ATPase from a “Purified” Light Density, “Canalicular”‐Enriched Plasma Membrane Fraction from Rat Liver. Hepatology 1983, 3: 18-28. PMID: 6130040, DOI: 10.1002/hep.1840030103.Peer-Reviewed Original Research