1992
Anion channels in rat liver canalicular plasma membranes reconstituted into planar lipid bilayers
Sellinger M, Weinman S, Henderson R, Zweifach A, Boyer J, Graf J. Anion channels in rat liver canalicular plasma membranes reconstituted into planar lipid bilayers. American Journal Of Physiology 1992, 262: g1027-g1032. PMID: 1377450, DOI: 10.1152/ajpgi.1992.262.6.g1027.Peer-Reviewed Original ResearchRegulatory volume decrease stimulates bile flow, bile acid excretion, and exocytosis in isolated perfused rat liver
Bruck R, Haddad P, Graf J, Boyer J. Regulatory volume decrease stimulates bile flow, bile acid excretion, and exocytosis in isolated perfused rat liver. American Journal Of Physiology 1992, 262: g806-g812. PMID: 1590390, DOI: 10.1152/ajpgi.1992.262.5.g806.Peer-Reviewed Original ResearchEffects of Ca2+ agonists on cytosolic Ca2+ in isolated hepatocytes and on bile secretion in the isolated perfused rat liver
Nathanson M, Gautam A, Bruck R, Isales C, Boyer J. Effects of Ca2+ agonists on cytosolic Ca2+ in isolated hepatocytes and on bile secretion in the isolated perfused rat liver. Hepatology 1992, 15: 107-116. PMID: 1727785, DOI: 10.1002/hep.1840150119.Peer-Reviewed Original ResearchMeSH KeywordsAequorinAnimalsBileCalcimycinCalciumCell SeparationCytosolHydroquinonesIn Vitro TechniquesLiverMalePressureRatsRats, Inbred StrainsVasopressinsConceptsBile flowPerfusion pressureBile secretionCytosolic Ca2Rat liverProtein kinase CIonophore-induced increaseBromo-A23187Hepatic vasoconstrictionVasodilator papaverineProtein kinase C inhibitor HExtracellular Ca2Kinase CSecretionLiverVasopressinAgonist vasopressinBenzohydroquinoneEffect of Ca2HepatocytesActivate protein kinase CInhibitor HTwo-thirdsIsolated hepatocytesSensitive photoprotein aequorin
1991
Cl(-)-HCO3- exchanger in isolated rat hepatocytes: role in regulation of intracellular pH
Benedetti A, Strazzabosco M, Corasanti J, Haddad P, Graf J, Boyer J. Cl(-)-HCO3- exchanger in isolated rat hepatocytes: role in regulation of intracellular pH. American Journal Of Physiology 1991, 261: g512-g522. PMID: 1887897, DOI: 10.1152/ajpgi.1991.261.3.g512.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAmmoniaAnimalsBariumBicarbonatesCarrier ProteinsCells, CulturedChloride-Bicarbonate AntiportersChloridesHydrogen-Ion ConcentrationIntracellular FluidKineticsLiverMaleRatsRats, Inbred StrainsConceptsAlkaline loadPH-sensitive dye BCECFMM H2DIDSPHi recoveryRegulation of intracellularRat hepatocyte monolayersAcid extrudersMM DIDSRat hepatocytesElectrophysiological techniquesDye BCECFHCO3- exchangerIsolated rat hepatocytesConstant pHoIntracellularMembrane potentialHepatocyte monolayersHepatocytesAcid loadingRole of chloride ions in liver cell volume regulation
Haddad P, Beck J, Boyer J, Graf J. Role of chloride ions in liver cell volume regulation. American Journal Of Physiology 1991, 261: g340-g348. PMID: 1872402, DOI: 10.1152/ajpgi.1991.261.2.g340.Peer-Reviewed Original ResearchConceptsRegulatory volume decreaseM omegaLiver cellsSingle isolated rat hepatocytesBiphasic responseHypotonic stressInitial hyperpolarizationPl/minIsolated rat hepatocytesGradual depolarizationCell volume regulationExternal ClRole of C1Hypotonic swellingVolume regulationMembrane potentialFacilitated releaseRat hepatocytesCellsMinVolume decreaseCell volumeInitial volumeInitial rateLiverEffect of UDCA on intracellular and biliary pH in isolated rat hepatocyte couplets and perfused livers
Strazzabosco M, Sakisaka S, Hayakawa T, Boyer J. Effect of UDCA on intracellular and biliary pH in isolated rat hepatocyte couplets and perfused livers. American Journal Of Physiology 1991, 260: g58-g69. PMID: 1987808, DOI: 10.1152/ajpgi.1991.260.1.g58.Peer-Reviewed Original ResearchConceptsBiliary HCO3- concentrationUrsodeoxycholic acidRat hepatocyte coupletsBiliary pHAcid loadEffect of UDCABile duct lumenHepatocyte coupletsIsolated rat hepatocyte coupletsBiliary epitheliumHCO3- concentrationDuct lumenRemoval of ClHCO3- exchangeRat liverLiverIPRLHepatocytesHypercholeresisPerfusateBileExcretionEpitheliumAmiloride
1990
DBcAMP stimulates vesicle transport and HRP excretion in isolated perfused rat liver
Hayakawa T, Bruck R, Ng O, Boyer J. DBcAMP stimulates vesicle transport and HRP excretion in isolated perfused rat liver. American Journal Of Physiology 1990, 259: g727-g735. PMID: 2173414, DOI: 10.1152/ajpgi.1990.259.5.g727.Peer-Reviewed Original ResearchConceptsBiliary excretionBile acidsBile acid excretionLiver 1Effects of adenosineRat liverElectron microscopic morphometric analysisTranscytotic vesicle pathwayLate peakHRP excretionHorseradish peroxidaseMin infusionBile flowExcretory functionAcid excretionO-dibutyryl cAMPPrimary hepatocyte culturesExcretionLiverDbcAMPCyclic monophosphateInfusionPericanalicular areaTaurocholate stimulates transcytotic vesicular pathways labeled by horseradish peroxidase in the isolated perfused rat liver
Hayakawa T, Cheng O, Ma A, Boyer J. Taurocholate stimulates transcytotic vesicular pathways labeled by horseradish peroxidase in the isolated perfused rat liver. Gastroenterology 1990, 99: 216-228. PMID: 2344927, DOI: 10.1016/0016-5085(90)91251-z.Peer-Reviewed Original ResearchCryptic na+, k+‐atpase activity in rat liver canalicular plasma membranes: Evidence for its basolateral origin
Sellinger M, Barrett C, Malle P, Gordon E, Boyer J. Cryptic na+, k+‐atpase activity in rat liver canalicular plasma membranes: Evidence for its basolateral origin. Hepatology 1990, 11: 223-229. PMID: 2155168, DOI: 10.1002/hep.1840110211.Peer-Reviewed Original ResearchConceptsFree-flow electrophoresisMembrane-fluidizing agentSurface chargeBasolateral enzymeBasolateral marker enzymeSmall contaminantsRat liver canalicular plasma membranesSmall amountRoutine assayAnodeSucrose density centrifugationMembraneElectrophoresisCanalicular liver plasma membranesEnzymeMembrane preparationsContaminantsPreparationChargeCanalicular markerPrecipitationLiver plasma membranesFractionActivityEffects of osmotic stresses on isolated rat hepatocytes. I. Ionic mechanisms of cell volume regulation
Corasanti J, Gleeson D, Boyer J. Effects of osmotic stresses on isolated rat hepatocytes. I. Ionic mechanisms of cell volume regulation. American Journal Of Physiology 1990, 258: g290-g298. PMID: 2305895, DOI: 10.1152/ajpgi.1990.258.2.g290.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAmilorideAnimalsBariumBicarbonatesBumetanideCells, CulturedChloridesHypertonic SolutionsHypotonic SolutionsKineticsLiverMaleOsmolar ConcentrationPotassium ChlorideQuinineRatsRats, Inbred StrainsSodium ChlorideEffects of osmotic stresses on isolated rat hepatocytes. II. Modulation of intracellular pH
Gleeson D, Corasanti J, Boyer J. Effects of osmotic stresses on isolated rat hepatocytes. II. Modulation of intracellular pH. American Journal Of Physiology 1990, 258: g299-g307. PMID: 2305896, DOI: 10.1152/ajpgi.1990.258.2.g299.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAnimalsBicarbonatesBuffersCells, CulturedCholineHydrogen-Ion ConcentrationHypertonic SolutionsHypotonic SolutionsIntracellular FluidKineticsLiverMaleOsmolar ConcentrationRatsRats, Inbred StrainsSodiumConceptsRegulatory volume decreaseAbsence of HCO3Presence of HCO3Acid-base transport systemsModulation of intracellularRat hepatocytesVolume regulationAcute withdrawalMM amilorideGreater intracellularHCO3- exchangeAcid effluxIsotonic mediumIsolated rat hepatocytesSame maneuverCell volume regulationHepatocytesNet acid effluxAmilorideSubconfluent monolayersDisulfonic acidIntracellularVolume decreaseHypotonic mediumHypertonic medium[34] Preparation of basolateral (sinusoidal) and canalicular plasma membrane vesicles for the study of hepatic transport processes
Meier P, Boyer J. [34] Preparation of basolateral (sinusoidal) and canalicular plasma membrane vesicles for the study of hepatic transport processes. Methods In Enzymology 1990, 192: 534-545. PMID: 1963666, DOI: 10.1016/0076-6879(90)92092-r.Peer-Reviewed Original Research
1989
Protein kinase C agonists inhibit bile secretion independently of effects on the microcirculation in the isolated perfused rat liver
Corasanti J, Smith N, Gordon E, Boyer J. Protein kinase C agonists inhibit bile secretion independently of effects on the microcirculation in the isolated perfused rat liver. Hepatology 1989, 10: 8-13. PMID: 2737605, DOI: 10.1002/hep.1840100103.Peer-Reviewed Original ResearchConceptsBile flowPerfusion pressureBile secretionVehicle dimethyl sulfoxideReversible decreaseConstant perfusate flowPhorbol dibutyrateVenous effluent concentrationsInactive phorbol ester 4Rat liver systemVascular redistributionHepatic microvasculaturePhorbol esterPhorbol ester 4Vasoactive agentsRole of hormonesPerfusate flowTrypan blue dyeAntagonist HProtein kinase C agonistsGm liverActive phorbol estersLiver systemRat liverOxygen consumptionBicarbonate-dependent and -independent intracellular pH regulatory mechanisms in rat hepatocytes. Evidence for Na+-HCO3- cotransport.
Gleeson D, Smith N, Boyer J. Bicarbonate-dependent and -independent intracellular pH regulatory mechanisms in rat hepatocytes. Evidence for Na+-HCO3- cotransport. Journal Of Clinical Investigation 1989, 84: 312-321. PMID: 2544626, PMCID: PMC303984, DOI: 10.1172/jci114156.Peer-Reviewed Original ResearchConceptsDisulfonic acid stilbeneHCO3- cotransportIntracellular acid loadHCO3- thanIntracellular pH regulatory mechanismsPH regulatory mechanismsBaseline pHiMonolayer cell culture systemRat hepatocytesAcid loadIntracellular ClCl-/HCO3AmilorideCell culture systemAnion transport inhibitorAbsence of bicarbonateHCO3- transportImportant regulatorHepatocytesPH-sensitive dyeCarboxy-fluoresceinTransport inhibitorsCulture systemRegulatory mechanismsPhiInward-rectifying potassium channels in rat hepatocytes
Henderson R, Graf J, Boyer J. Inward-rectifying potassium channels in rat hepatocytes. American Journal Of Physiology 1989, 256: g1028-g1035. PMID: 2735409, DOI: 10.1152/ajpgi.1989.256.6.g1028.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedElectric ConductivityKineticsLiverMaleMembrane PotentialsPotassium ChannelsRatsRats, Inbred StrainsConceptsRenal epithelial cellsRat hepatocytesWhole-cell conductanceWhole-cell experimentsSingle-channel recordingsEpithelial cellsPotassium channelsHigh intracellularFunctional heterogeneityOpen probabilityIsolated rat hepatocytesCellsWhole-cell modeInward rectificationMarked similarityStrong inward rectificationCell conductanceHepatocytesConductanceCalcium dependencePatch-clamp techniqueCurrent-voltage relationshipInward conductanceSpeciesCell experimentsVoltage-driven, taurocholate-dependent secretion in isolated hepatocyte couplets
Weinman S, Graf J, Boyer J. Voltage-driven, taurocholate-dependent secretion in isolated hepatocyte couplets. American Journal Of Physiology 1989, 256: g826-g832. PMID: 2719107, DOI: 10.1152/ajpgi.1989.256.5.g826.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBileBile CanaliculiBiological TransportIn Vitro TechniquesLiverMaleMembrane PotentialsRatsRats, Inbred StrainsTaurocholic AcidConceptsBile secretionBile acidsSecretion rateFluid secretionIntracellular current injectionCanalicular areaHepatocyte coupletsMembrane potentialRat hepatocyte coupletsAbsence of taurocholateMicroM taurocholateFluid secretion rateBile formationSecretionCarrier-mediated transportTaurocholateLiverIntracellular voltageHepatic immunohistochemical localization of the tight junction protein ZO-1 in rat models of cholestasis.
Anderson J, Glade J, Stevenson B, Boyer J, Mooseker M. Hepatic immunohistochemical localization of the tight junction protein ZO-1 in rat models of cholestasis. American Journal Of Pathology 1989, 134: 1055-62. PMID: 2719075, PMCID: PMC1879891.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCholestasisImmunohistochemistryIntercellular JunctionsLiverMaleMembrane ProteinsPhosphoproteinsRatsRats, Inbred StrainsZonula Occludens-1 ProteinConceptsZO-1 proteinZO-1Tight junctionsConsecutive daily subcutaneous injectionsFrozen sectionsReflux of bileDaily subcutaneous injectionsBile duct obstructionCommon bile ductMale Sprague-DawleyHepatocyte tight junctionsBile duct ligation resultsTight junction protein ZO-1Cholestatic modelsDuct obstructionBile ductSubcutaneous injectionCholestatic liverRat modelEthinyl estradiolSprague-DawleyProtein ZO-1Immunoperoxidase stainingZO-1 stainingImmunohistochemical localizationDeterminants of glutathione efflux and biliary GSH/GSSG ratio in perfused rat liver
Ballatori N, Truong A, K. A, Boyer J. Determinants of glutathione efflux and biliary GSH/GSSG ratio in perfused rat liver. American Journal Of Physiology 1989, 256: g482-g490. PMID: 2564253, DOI: 10.1152/ajpgi.1989.256.3.g482.Peer-Reviewed Original ResearchConceptsGamma-glutamyl transferase activityO2 deliveryBiliary glutathioneHepatic effluxSinusoidal effluxKrebs-Ringer bicarbonate bufferGlutathione effluxRat liverEfflux of glutathioneGSH/GSSG ratioRed blood cellsPerfusion flow rateFluorocarbon emulsionsBile flowTissue GSHOxygen deliveryTransferase activityAT-125LiverBovine red blood cellsPerfusate compositionBlood cellsBileGSSG levelsGSSG ratioQuantitative assessment of canalicular bile formation in isolated hepatocyte couplets using microscopic optical planimetry.
Gautam A, Ng O, Strazzabosco M, Boyer J. Quantitative assessment of canalicular bile formation in isolated hepatocyte couplets using microscopic optical planimetry. Journal Of Clinical Investigation 1989, 83: 565-573. PMID: 2913052, PMCID: PMC303716, DOI: 10.1172/jci113919.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBileBile CanaliculiBile Ducts, IntrahepaticLiverMicroscopy, ElectronRatsRats, Inbred StrainsTaurocholic AcidUrsodeoxycholic AcidConceptsIsolated rat hepatocyte couplets
1988
Tubulovesicular Transcytotic Pathway in Isolated Rat Hepatocyte Couplets in Culture Effect of Colchicine and Taurocholate
Sakisaka S, Ng O, Boyer J. Tubulovesicular Transcytotic Pathway in Isolated Rat Hepatocyte Couplets in Culture Effect of Colchicine and Taurocholate. Gastroenterology 1988, 95: 793-804. PMID: 3396824, DOI: 10.1016/s0016-5085(88)80030-1.Peer-Reviewed Original Research