Imaging and optogenetic modulation of vascular mural cells in the live brain
Tong L, Hill RA, Damisah EC, Murray KN, Yuan P, Bordey A, Grutzendler J. Imaging and optogenetic modulation of vascular mural cells in the live brain. Nature Protocols 2020, 16: 472-496. PMID: 33299155, DOI: 10.1038/s41596-020-00425-w.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsRegional cerebral blood flowMural cellsBlood-brain barrier maintenanceCerebral ischemia mouse modelAge-related neurodegenerative diseasesCerebral blood flowSmooth muscle cell physiologyBrain blood vesselsIschemia mouse modelVascular mural cellsBrain microvesselsHigh-resolution intravital imagingVascular disordersMouse modelBlood flowMuscle cell physiologyTransgenic miceCalcium transientsAlzheimer's diseaseCalcium imagingCell subtypesBarrier maintenanceNeurodegenerative diseasesTwo-photon optogeneticsBlood vesselsLong-term dendritic spine stability in the adult cortex
Grutzendler J, Kasthuri N, Gan WB. Long-term dendritic spine stability in the adult cortex. Nature 2002, 420: 812-816. PMID: 12490949, DOI: 10.1038/nature01276.Peer-Reviewed Original ResearchConceptsLayer 5 pyramidal neuronsAxo-dendritic synapsesFilopodia-like dendritic protrusionsPrimary visual cortexVisual cortex developmentDendritic spine stabilityTwo-photon imaging techniquePyramidal neuronsDendritic specializationsAdult cortexYoung miceSpine eliminationAdult miceOne-month intervalsNervous systemTransgenic miceSpine stabilityVisual cortexDendritic protrusionsMammalian brainCortex developmentAdult animalsSpineMiceYoung animals