Featured Publications
IL‐1β Drives Production of FGF‐23 at the Onset of Chronic Kidney Disease in Mice
McKnight Q, Jenkins S, Li X, Nelson T, Marlier A, Cantley LG, Finberg KE, Fretz JA. IL‐1β Drives Production of FGF‐23 at the Onset of Chronic Kidney Disease in Mice. Journal Of Bone And Mineral Research 2020, 35: 1352-1362. PMID: 32154933, PMCID: PMC7363582, DOI: 10.1002/jbmr.4003.Peer-Reviewed Original ResearchConceptsChronic kidney diseaseOnset of CKDEarly chronic kidney diseaseFGF-23 expressionFGF-23Renal dysfunctionParathyroid hormoneIL-1βCongenital chronic kidney diseaseFGF-23 levelsSerum parathyroid hormoneGlomerular capillary tuftCongenital modelSerum phosphateIron bioavailabilitySystemic elevationVitamin DInflammatory cytokinesKidney diseaseEarly biomarkersIron statusMouse modelPhosphate imbalanceInitial upregulationCapillary tuft
2020
Reporting Guidelines, Review of Methodological Standards, and Challenges Toward Harmonization in Bone Marrow Adiposity Research. Report of the Methodologies Working Group of the International Bone Marrow Adiposity Society
Tratwal J, Labella R, Bravenboer N, Kerckhofs G, Douni E, Scheller EL, Badr S, Karampinos DC, Beck-Cormier S, Palmisano B, Poloni A, Moreno-Aliaga MJ, Fretz J, Rodeheffer MS, Boroumand P, Rosen CJ, Horowitz MC, van der Eerden BCJ, Veldhuis-Vlug AG, Naveiras O. Reporting Guidelines, Review of Methodological Standards, and Challenges Toward Harmonization in Bone Marrow Adiposity Research. Report of the Methodologies Working Group of the International Bone Marrow Adiposity Society. Frontiers In Endocrinology 2020, 11: 65. PMID: 32180758, PMCID: PMC7059536, DOI: 10.3389/fendo.2020.00065.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsBone marrow adiposityBone marrow adipocytesMarrow adipocytesBone marrow adipogenesisSpecific MRI sequencesMarrow adiposityMarrow adipogenesisAnimal modelsRange of diseasesContrast-enhanced μCTStromal cell precursorsStudy outcomesConsensus opinionGene panelCell precursorsGold standardRT-qPCRMRI sequencesPotential roleMinimal reporting standardsPrecise reportingHistomorphometry
2017
Bone marrow adipocytes
Horowitz MC, Berry R, Holtrup B, Sebo Z, Nelson T, Fretz JA, Lindskog D, Kaplan JL, Ables G, Rodeheffer MS, Rosen CJ. Bone marrow adipocytes. Adipocyte 2017, 6: 193-204. PMID: 28872979, PMCID: PMC5638373, DOI: 10.1080/21623945.2017.1367881.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsBone marrow adipocytesBone marrowMarrow adipocytesDistinct adipose depotsAdipose tissue increasesWhole-body metabolismHuman bone marrowAdipose depotsAdipose tissueBeige adipocytesBody metabolismMarrowLittle functional significanceFunctional significanceAdipocytesCell populationsTissue increasesInduction signalRecent dataCellsAdipokines
2014
Early B Cell Factor 1 Regulates Adipocyte Morphology and Lipolysis in White Adipose Tissue
Gao H, Mejhert N, Fretz JA, Arner E, Lorente-Cebrián S, Ehrlund A, Dahlman-Wright K, Gong X, Strömblad S, Douagi I, Laurencikiene J, Dahlman I, Daub CO, Rydén M, Horowitz MC, Arner P. Early B Cell Factor 1 Regulates Adipocyte Morphology and Lipolysis in White Adipose Tissue. Cell Metabolism 2014, 19: 981-992. PMID: 24856929, PMCID: PMC4109056, DOI: 10.1016/j.cmet.2014.03.032.Peer-Reviewed Original ResearchAdipocytesAdipogenesisAdipose Tissue, WhiteAdiposityAnimalsCells, CulturedDiabetes MellitusDiet, High-FatFemaleGene ExpressionHumansHypertrophyInflammationInsulin ResistanceLipolysisMaleMiceMice, Inbred C57BLMice, KnockoutRNA InterferenceRNA, Small InterferingTrans-ActivatorsTumor Necrosis Factor-alphaChapter Seven Use of Osmium Tetroxide Staining with Microcomputerized Tomography to Visualize and Quantify Bone Marrow Adipose Tissue In Vivo
Scheller EL, Troiano N, VanHoutan JN, Bouxsein MA, Fretz JA, Xi Y, Nelson T, Katz G, Berry R, Church CD, Doucette CR, Rodeheffer MS, MacDougald OA, Rosen CJ, Horowitz MC. Chapter Seven Use of Osmium Tetroxide Staining with Microcomputerized Tomography to Visualize and Quantify Bone Marrow Adipose Tissue In Vivo. Methods In Enzymology 2014, 537: 123-139. PMID: 24480344, PMCID: PMC4097010, DOI: 10.1016/b978-0-12-411619-1.00007-0.Peer-Reviewed Original ResearchConceptsStromal vascular fractionBone marrowAdipose tissueBone marrow adipose tissueWhite adipose tissue depotsMicrocomputerized tomographyAdipose tissue depotsMarrow recoveryDrug treatmentMedullary canalTissue depotsMetabolic diseasesVascular fractionStromal cellsMarrow fatEndothelial cellsAdipocyte progenitorsMature adipocytesLong bonesMAT volumeAdipocytesStainingOsmium tetroxide stainingHistochemical stainingConventional quantitation
2011
Adipocyte Lineage Cells Contribute to the Skin Stem Cell Niche to Drive Hair Cycling
Festa E, Fretz J, Berry R, Schmidt B, Rodeheffer M, Horowitz M, Horsley V. Adipocyte Lineage Cells Contribute to the Skin Stem Cell Niche to Drive Hair Cycling. Cell 2011, 146: 761-771. PMID: 21884937, PMCID: PMC3298746, DOI: 10.1016/j.cell.2011.07.019.Peer-Reviewed Original ResearchConceptsAdipocyte lineage cellsStem cell nicheStem cell activityLineage cellsCell nicheSkin stem cell nichesStem cell functionEpithelial stem cell nicheStem cell activationEpithelial stem cell functionSkin stem cellsTissue homeostasisNiche cellsFunctional analysisAdipogenic cellsSkin homeostasisMammalian skinTransplantation experimentsStem cellsPrecursor cellsHair cyclingCell functionAdipocyte cellsNicheFunctional tissue
2010
How B cells influence bone biology in health and disease
Horowitz MC, Fretz JA, Lorenzo JA. How B cells influence bone biology in health and disease. Bone 2010, 47: 472-479. PMID: 20601290, PMCID: PMC2941392, DOI: 10.1016/j.bone.2010.06.011.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsHematopoietic stem cellsB cell differentiationCell differentiationTranscription factorsBone cellsImportant regulatory interactionsBone phenotypeRegulatory interactionsB cellsTemporal mannerSupportive nicheStem cellsAdaptive immune systemBone biologyDifferentiationRANKL-RANKActivated B cellsEndosteal bone surfaceNormal bone remodelingNicheSpecific responsesPhenotypeCellsImportant roleImmune system
2009
Regulation of Aryl Hydrocarbon Receptor Function by Selective Estrogen Receptor Modulators
DuSell CD, Nelson ER, Wittmann BM, Fretz JA, Kazmin D, Thomas RS, Pike JW, McDonnell DP. Regulation of Aryl Hydrocarbon Receptor Function by Selective Estrogen Receptor Modulators. Endocrinology 2009, 24: 33-46. PMID: 19901195, PMCID: PMC2802893, DOI: 10.1210/me.2009-0339.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAryl Hydrocarbon Receptor Nuclear TranslocatorBreast NeoplasmsCell DifferentiationCell LineCell Line, TumorChromatin ImmunoprecipitationDose-Response Relationship, DrugFemaleGene Expression ProfilingGene Expression RegulationHumansMaleMiceOsteoclastsReceptors, Aryl HydrocarbonReceptors, EstrogenRecombinant ProteinsSelective Estrogen Receptor ModulatorsTamoxifenConceptsSelective estrogen receptor modulatorsAryl hydrocarbon receptorEstrogen receptor modulatorsEstrogen receptorReceptor modulatorsBreast cancerAbsence of ERER-independent mannerAryl hydrocarbon receptor functionAgonist/antagonist activityActivity of drugsAhR target genesEstradiol metabolismPharmacological actionsOsteoclast differentiationTamoxifenTherapeutic efficacyActive metaboliteReceptor functionAntagonist activityHydrocarbon receptorCalcium signalingCellular proliferationPotential roleCellular model
2007
Molecular Actions of 1,25‐Dihydroxyvitamin D3 on Genes Involved in Calcium Homeostasis*
Pike JW, Zella LA, Meyer MB, Fretz JA, Kim S. Molecular Actions of 1,25‐Dihydroxyvitamin D3 on Genes Involved in Calcium Homeostasis*. Journal Of Bone And Mineral Research 2007, 22: v16-v19. PMID: 18290714, DOI: 10.1359/jbmr.07s207.Peer-Reviewed Original ResearchConceptsTranscriptional start siteRegulatory regionsExpression of TRPV6RANKL geneKey regulatory regionsVertebrate organismsNovel genesChromatin immunoprecipitationStart siteRegulatory elementsUpstream sequencesGene expressionEnhancer regionMolecular mechanismsHuman TRPV6Upstream regionGenesTRPV6 geneMolecular actionsMolecular techniquesPrimary regulatorMicroarray methodKbTRPV6Expression1,25-Dihydroxyvitamin D3 induces expression of the Wnt signaling co-regulator LRP5 via regulatory elements located significantly downstream of the gene's transcriptional start site
Fretz JA, Zella LA, Kim S, Shevde NK, Pike JW. 1,25-Dihydroxyvitamin D3 induces expression of the Wnt signaling co-regulator LRP5 via regulatory elements located significantly downstream of the gene's transcriptional start site. The Journal Of Steroid Biochemistry And Molecular Biology 2007, 103: 440-445. PMID: 17229572, PMCID: PMC1868540, DOI: 10.1016/j.jsbmb.2006.11.018.Peer-Reviewed Original ResearchConceptsTranscriptional start siteGene transcriptional start siteChIP-chip analysisLRP5/LRP6Chromatin structureHuman genomeRegulatory regionsStart siteRegulatory elementsWnt familyHeterologous promoterCanonical WntMembrane receptorsMRNA transcriptsWntSecreted memberOsteoblastic cellsLRP5GenesVDRELRP5 geneBone formationProteinLrp5 locusOsteoblast numberPerspectives on mechanisms of gene regulation by 1,25-dihydroxyvitamin D3 and its receptor
Pike JW, Meyer MB, Watanuki M, Kim S, Zella LA, Fretz JA, Yamazaki M, Shevde NK. Perspectives on mechanisms of gene regulation by 1,25-dihydroxyvitamin D3 and its receptor. The Journal Of Steroid Biochemistry And Molecular Biology 2007, 103: 389-395. PMID: 17223545, PMCID: PMC1868541, DOI: 10.1016/j.jsbmb.2006.12.050.Peer-Reviewed Original ResearchConceptsTarget genesBasal transcriptional machineryChromatin immunoprecipitation techniqueVitamin D receptorKey target genesExpression of genesAltered gene expressionTranscriptional machineryVertebrate organismsGene regulationSystemic signalsTranscriptional modulationRegulatory regionsDNA sitesGene locusGene expressionPromoter regionEnhancer regionGenesImmunoprecipitation techniquesRegulatory capabilitiesMaintenance of calciumModular natureExpressionNew insightsMultiple enhancer regions located at significant distances upstream of the transcriptional start site mediate RANKL gene expression in response to 1,25-dihydroxyvitamin D3
Kim S, Yamazaki M, Zella LA, Meyer MB, Fretz JA, Shevde NK, Pike JW. Multiple enhancer regions located at significant distances upstream of the transcriptional start site mediate RANKL gene expression in response to 1,25-dihydroxyvitamin D3. The Journal Of Steroid Biochemistry And Molecular Biology 2007, 103: 430-434. PMID: 17197168, PMCID: PMC1892901, DOI: 10.1016/j.jsbmb.2006.12.020.Peer-Reviewed Original ResearchConceptsTranscription start siteStart siteChip analysisMultiple enhancer regionsRNA polymerase IIPotential regulatory regionsTranscriptional start siteRANKL geneVDR/RXRChromatin hubHistone modificationsTranscriptional outputPolymerase IIRANKL gene expressionImportant functional consequencesMultiple enhancersRegulatory regionsTranscription factorsHeterologous promoterGene locusEnhancer regionGene expressionKb upstreamRANKL promoterLuciferase assay
2006
1,25-Dihydroxyvitamin D3 regulates the expression of low-density lipoprotein receptor-related protein 5 via deoxyribonucleic acid sequence elements located downstream of the start site of transcription.
Fretz JA, Zella LA, Kim S, Shevde NK, Pike JW. 1,25-Dihydroxyvitamin D3 regulates the expression of low-density lipoprotein receptor-related protein 5 via deoxyribonucleic acid sequence elements located downstream of the start site of transcription. Endocrinology 2006, 20: 2215-30. PMID: 16613987, DOI: 10.1210/me.2006-0102.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone and BonesCells, CulturedChromatinChromatin ImmunoprecipitationConserved SequenceDimerizationDNAGene Expression RegulationHumansIntronsLDL-Receptor Related ProteinsLow Density Lipoprotein Receptor-Related Protein-5MiceMice, Inbred C57BLMolecular Sequence DataOsteoblastsPromoter Regions, GeneticProtein BindingReceptors, CalcitriolRetinoid X ReceptorsRNA Polymerase IISequence AlignmentTranscription, GeneticVitamin DVitamin D Response ElementConceptsVitamin D response elementD response elementResponse elementDNA microarray analysisChromatin structureChromatin immunoprecipitationOsteoblast biologyReceptor FrizzledHuman genomeLipoprotein receptor-related protein 5Regulatory regionsStart siteLrp5 locusLow-density lipoprotein receptor-related protein 5Sequence elementsHeterologous promoterMature osteoblastsMicroarray analysisOsteoblast precursorsDirect targetProtein 5Cell line originLine originPrimary osteoblastsGenes