2010
How B cells influence bone biology in health and disease
Horowitz MC, Fretz JA, Lorenzo JA. How B cells influence bone biology in health and disease. Bone 2010, 47: 472-479. PMID: 20601290, PMCID: PMC2941392, DOI: 10.1016/j.bone.2010.06.011.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsHematopoietic stem cellsB cell differentiationCell differentiationTranscription factorsBone cellsImportant regulatory interactionsBone phenotypeRegulatory interactionsB cellsTemporal mannerSupportive nicheStem cellsAdaptive immune systemBone biologyDifferentiationRANKL-RANKActivated B cellsEndosteal bone surfaceNormal bone remodelingNicheSpecific responsesPhenotypeCellsImportant roleImmune system
2007
Receptor Activator of Nuclear Factor-κB Ligand-Induced Nuclear Factor of Activated T Cells (C1) Autoregulates Its Own Expression in Osteoclasts and Mediates the Up-Regulation of Tartrate-Resistant Acid Phosphatase
Fretz JA, Shevde NK, Singh S, Darnay BG, Pike JW. Receptor Activator of Nuclear Factor-κB Ligand-Induced Nuclear Factor of Activated T Cells (C1) Autoregulates Its Own Expression in Osteoclasts and Mediates the Up-Regulation of Tartrate-Resistant Acid Phosphatase. Endocrinology 2007, 22: 737-750. PMID: 18063694, PMCID: PMC2262172, DOI: 10.1210/me.2007-0333.Peer-Reviewed Original ResearchMeSH KeywordsAcid PhosphataseAnimalsBlotting, WesternBone and BonesCell LineChromatin ImmunoprecipitationHomeostasisIsoenzymesMiceMice, Inbred C57BLNFATC Transcription FactorsOsteoclastsPromoter Regions, GeneticRANK LigandReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTartrate-Resistant Acid PhosphataseTranscription, GeneticUp-RegulationConceptsNFAT membersRNA polymerase IIDNA-binding proteinsSpecific transcription factorsChromatin immunoprecipitation analysisSignal transduction pathwaysAdditional molecular detailsNuclear factorActivated T cells cytoplasmic 1Polymerase IIAcp5 promotersTranscription factorsTransduction pathwaysMolecular detailsTarget genesOwn expressionImmunoprecipitation analysisP1 promoterBone-resorbing cellsReceptor activatorHematopoietic precursorsGenesNuclear factor-κB ligandCytoplasmic 1Time-dependent accumulationMolecular Actions of 1,25‐Dihydroxyvitamin D3 on Genes Involved in Calcium Homeostasis*
Pike JW, Zella LA, Meyer MB, Fretz JA, Kim S. Molecular Actions of 1,25‐Dihydroxyvitamin D3 on Genes Involved in Calcium Homeostasis*. Journal Of Bone And Mineral Research 2007, 22: v16-v19. PMID: 18290714, DOI: 10.1359/jbmr.07s207.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumHomeostasisHumansRANK LigandTRPV Cation ChannelsVitamin DVitamin D Response ElementConceptsTranscriptional start siteRegulatory regionsExpression of TRPV6RANKL geneKey regulatory regionsVertebrate organismsNovel genesChromatin immunoprecipitationStart siteRegulatory elementsUpstream sequencesGene expressionEnhancer regionMolecular mechanismsHuman TRPV6Upstream regionGenesTRPV6 geneMolecular actionsMolecular techniquesPrimary regulatorMicroarray methodKbTRPV6ExpressionTargeted Deletion of a Distant Transcriptional Enhancer of the Receptor Activator of Nuclear Factor-κB Ligand Gene Reduces Bone Remodeling and Increases Bone Mass
Galli C, Zella LA, Fretz JA, Fu Q, Pike JW, Weinstein RS, Manolagas SC, O’Brien C. Targeted Deletion of a Distant Transcriptional Enhancer of the Receptor Activator of Nuclear Factor-κB Ligand Gene Reduces Bone Remodeling and Increases Bone Mass. Endocrinology 2007, 149: 146-153. PMID: 17932217, PMCID: PMC2194617, DOI: 10.1210/en.2007-0734.Peer-Reviewed Original ResearchPerspectives on mechanisms of gene regulation by 1,25-dihydroxyvitamin D3 and its receptor
Pike JW, Meyer MB, Watanuki M, Kim S, Zella LA, Fretz JA, Yamazaki M, Shevde NK. Perspectives on mechanisms of gene regulation by 1,25-dihydroxyvitamin D3 and its receptor. The Journal Of Steroid Biochemistry And Molecular Biology 2007, 103: 389-395. PMID: 17223545, PMCID: PMC1868541, DOI: 10.1016/j.jsbmb.2006.12.050.Peer-Reviewed Original ResearchConceptsTarget genesBasal transcriptional machineryChromatin immunoprecipitation techniqueVitamin D receptorKey target genesExpression of genesAltered gene expressionTranscriptional machineryVertebrate organismsGene regulationSystemic signalsTranscriptional modulationRegulatory regionsDNA sitesGene locusGene expressionPromoter regionEnhancer regionGenesImmunoprecipitation techniquesRegulatory capabilitiesMaintenance of calciumModular natureExpressionNew insightsMultiple enhancer regions located at significant distances upstream of the transcriptional start site mediate RANKL gene expression in response to 1,25-dihydroxyvitamin D3
Kim S, Yamazaki M, Zella LA, Meyer MB, Fretz JA, Shevde NK, Pike JW. Multiple enhancer regions located at significant distances upstream of the transcriptional start site mediate RANKL gene expression in response to 1,25-dihydroxyvitamin D3. The Journal Of Steroid Biochemistry And Molecular Biology 2007, 103: 430-434. PMID: 17197168, PMCID: PMC1892901, DOI: 10.1016/j.jsbmb.2006.12.020.Peer-Reviewed Original ResearchConceptsTranscription start siteStart siteChip analysisMultiple enhancer regionsRNA polymerase IIPotential regulatory regionsTranscriptional start siteRANKL geneVDR/RXRChromatin hubHistone modificationsTranscriptional outputPolymerase IIRANKL gene expressionImportant functional consequencesMultiple enhancersRegulatory regionsTranscription factorsHeterologous promoterGene locusEnhancer regionGene expressionKb upstreamRANKL promoterLuciferase assay