2021
Physiologically detectable bisphenol A impairs human sperm functions by reducing protein-tyrosine phosphorylation
Li N, Kang H, Peng Z, Wang H, Weng S, Zeng X. Physiologically detectable bisphenol A impairs human sperm functions by reducing protein-tyrosine phosphorylation. Ecotoxicology And Environmental Safety 2021, 221: 112418. PMID: 34146982, DOI: 10.1016/j.ecoenv.2021.112418.Peer-Reviewed Original ResearchConceptsHuman sperm viabilityHuman sperm functionHuman spermExposure of BPASperm functionProgesterone-induced acrosome reactionProtein tyrosine phosphorylationProtein tyrosine phosphorylation levelsAcrosome reactionMale infertilityHealthy peopleMouse sperm motilitySperm viabilityCalcium signalingPhosphorylation levelsTyrosine phosphorylation levelsBisphenol ASperm motilityProgressive motilityHyperactivationMotilityDetectable concentrationRemarkable declineTyrosine phosphorylationPlastic monomers
2020
Sperm ion channels and transporters in male fertility and infertility
Wang H, McGoldrick LL, Chung JJ. Sperm ion channels and transporters in male fertility and infertility. Nature Reviews Urology 2020, 18: 46-66. PMID: 33214707, PMCID: PMC7852504, DOI: 10.1038/s41585-020-00390-9.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsSperm ion channelsIon channelsSperm cellsMammalian sperm cellsIon signalingDynamic regulationDefective sperm functionFemale reproductive tractChannel CatSperHuman geneticsMembrane receptorsMale fertilityHealth careAttractive targetDirect electrophysiological recordingsOverall health careHuman infertilityReproductive health careSperm functionPrincipal Ca2Development of contraceptivesSperm activityGene variantsReproductive tractGenetic abnormalities
2017
Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in Mice
Wang H, Chang M, Peng T, Yang Y, Li N, Luo T, Cheng Y, Zhou M, Zeng X, Zheng L. Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in Mice. Cellular Physiology And Biochemistry 2017, 42: 44-54. PMID: 28554186, DOI: 10.1159/000477113.Peer-Reviewed Original ResearchConceptsAdult male C57BL/6 miceMale C57BL/6 miceImpair sperm functionAcute cadmium exposureSperm functionSperm-specific Ca2C57BL/6 miceReproductive toxicityCadmium exposureWestern blottingDifferent dosesSperm dysfunctionExpression levelsEnvironmental heavy metalsToxic effectsMiceEndocrine disruptorsCatSperSperm motilityMouse spermatozoaSpecific roleToxicityExposureMotilityDysfunction
2016
Diethylstilbestrol activates CatSper and disturbs progesterone actions in human spermatozoa
Zou Q, Peng Z, Zhao Q, Chen H, Cheng Y, Liu Q, He Y, Weng S, Wang H, Wang T, Zheng L, Luo T. Diethylstilbestrol activates CatSper and disturbs progesterone actions in human spermatozoa. Human Reproduction 2016, 32: 290-298. PMID: 28031325, DOI: 10.1093/humrep/dew332.Peer-Reviewed Original ResearchConceptsNon-genomic mannerProgesterone actionHuman sperm functionEndocrine-disrupting chemicalsSperm intracellular calcium concentrationsWhole-cell patch-clamp techniqueSperm functionHuman spermatozoaPARTICIPANTS/MATERIALSHuman sperm penetrationIndicator Fluo-4 AMIntracellular calcium concentrationROLE OF CHANCEAcrosome reactionEffects of diethylstilbestrolPatch-clamp techniqueFluo-4 AMFemale reproductive tract fluidsMale reproductive systemTyrosine phosphorylationReproductive tract fluidsDES exposurePostnatal exposureEstrogenic endocrine disruptorsAbility of spermBisphenol A Impairs Mature Sperm Functions by a CatSper-Relevant Mechanism
Wang H, Liu M, Li N, Luo T, Zheng L, Zeng X. Bisphenol A Impairs Mature Sperm Functions by a CatSper-Relevant Mechanism. Toxicological Sciences 2016, 152: 145-154. PMID: 27125968, DOI: 10.1093/toxsci/kfw070.Peer-Reviewed Original ResearchConceptsToxicity of BPANormal mouse spermSperm functionMature sperm functionVivo administrationEndocrine-disrupting chemicalsWestern blotDifferent dosesSignificant decreaseSperm total motilitySignificant reductionBisphenol AMiceSperm motilityMouse spermMouse spermatozoaMotilityCatSperTotal motilityToxicityMatrine compromises mouse sperm functions by a [Ca2+]i-related mechanism
Luo T, Zou Q, He Y, Wang H, Wang T, Liu M, Chen Y, Wang B. Matrine compromises mouse sperm functions by a [Ca2+]i-related mechanism. Reproductive Toxicology 2016, 60: 69-75. PMID: 26867864, DOI: 10.1016/j.reprotox.2016.02.003.Peer-Reviewed Original ResearchConceptsMouse sperm functionSperm functionDaily dosesIntraperitoneal injectionMale miceSperm countTestis weightReproductive toxicityMiceChinese medicineCatSper channelsMatrineProgressive motilityAcrosome reactionKey regulatorMouse spermTotal motilitySperm viabilityGene expressionTestis sizeMotilitySpermDoses
2015
Matrine Inhibits Mouse Sperm Function by Reducing Sperm [Ca2+]i and Phospho-ERK1/2
Luo T, Zou Q, He Y, Wang H, Li N, Zeng X. Matrine Inhibits Mouse Sperm Function by Reducing Sperm [Ca2+]i and Phospho-ERK1/2. Cellular Physiology And Biochemistry 2015, 35: 374-385. PMID: 25591778, DOI: 10.1159/000369703.Peer-Reviewed Original ResearchMeSH KeywordsAcrosome ReactionAlkaloidsAniline CompoundsAnimalsCalcimycinCalciumFemaleFertilization in VitroMaleMatrinesMiceMice, Inbred C57BLMicroscopy, FluorescenceMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Patch-Clamp TechniquesPhosphorylationPotassiumQuinolizinesSperm MotilitySpermatozoaXanthenesConceptsMouse sperm functionIntracellular calcium concentrationSperm functionCauda epididymal spermMouse spermPhosphorylation of ERK1/2Acrosome reaction rateEpididymal spermMouse cauda epididymal spermFertilization abilityPharmacological effectsPotassium currentP-ERK1/2Phospho-ERK1/2Calcium concentrationChinese medicineKinase 1/2Male reproductionSignificant reductionMatrineMature mouse spermProgressive motilityAcrosome reactionMotilityTotal motility
2013
Sperm-specific ion channels: targets holding the most potential for male contraceptives in development
Zheng L, Wang H, Li B, Zeng X. Sperm-specific ion channels: targets holding the most potential for male contraceptives in development. Contraception 2013, 88: 485-491. PMID: 23845210, DOI: 10.1016/j.contraception.2013.06.002.Peer-Reviewed Original ResearchMeSH KeywordsAnoctamin-1Calcium ChannelsChloride Channel AgonistsChloride ChannelsContraceptive Agents, MaleDrugs, InvestigationalHumansIon ChannelsLarge-Conductance Calcium-Activated Potassium Channel alpha SubunitsLarge-Conductance Calcium-Activated Potassium ChannelsMaleMembrane ProteinsNeoplasm ProteinsOrgan SpecificityPotassium Channels, Voltage-GatedProtein SubunitsSignal TransductionSpermatozoa