1993
Non-neurotoxic amphetamine derivatives release serotonin through serotonin transporters.
Rudnick G, Wall S. Non-neurotoxic amphetamine derivatives release serotonin through serotonin transporters. Molecular Pharmacology 1993, 43: 271-6. PMID: 8429828.Peer-Reviewed Original ResearchMeSH KeywordsAmphetaminesBlood PlateletsCarrier ProteinsCell MembraneChromaffin GranulesHumansImipramineIn Vitro TechniquesIndansMembrane GlycoproteinsMembrane Transport ProteinsMethamphetamineModels, BiologicalNerve EndingsNerve Tissue ProteinsRadioligand AssaySerotoninSerotonin Plasma Membrane Transport ProteinsConceptsChromaffin granule membrane vesiclesPlasma membrane vesiclesMembrane vesiclesPlatelet plasma membrane vesiclesPlasma membrane transportersSerotonin transportBiogenic amine transportersMembrane transportersAmine transportersTransportersVesiclesDopamine transporterSerotonin transporterModel systemHalf-maximal concentrationNerve terminalsTransmembraneCocaine analogBindingSimilar concentrationsHigh concentrationsCellsTransport
1992
p-Chloroamphetamine induces serotonin release through serotonin transporters.
Rudnick G, Wall S. p-Chloroamphetamine induces serotonin release through serotonin transporters. Biochemistry 1992, 31: 6710-8. PMID: 1322169, DOI: 10.1021/bi00144a010.Peer-Reviewed Original ResearchMeSH KeywordsBinding, CompetitiveBiological TransportBlood PlateletsCarrier ProteinsCell MembraneChloridesHumansHydrogen-Ion ConcentrationImipramineKineticsLithiumLithium ChlorideMembrane GlycoproteinsMembrane Transport ProteinsNerve Tissue ProteinsP-ChloroamphetamineRadioisotope Dilution TechniqueSerotoninSerotonin Plasma Membrane Transport ProteinsTritiumConceptsPlasma membrane vesiclesMembrane vesiclesChromaffin granule membrane vesiclesVesicular amine transporterATP hydrolysisBovine adrenal chromaffin granulesSerotonin transporterAdrenal chromaffin granulesPrevents accumulationAmine transportersPresence of Mg2TransportersVesiclesChromaffin granulesModel systemDelta pHHuman plateletsATPEffluxManner characteristicAccumulationTransmembraneNaCl gradientPCA's abilityP-chloroamphetamineThe molecular mechanism of "ecstasy" [3,4-methylenedioxy-methamphetamine (MDMA)]: serotonin transporters are targets for MDMA-induced serotonin release.
Rudnick G, Wall S. The molecular mechanism of "ecstasy" [3,4-methylenedioxy-methamphetamine (MDMA)]: serotonin transporters are targets for MDMA-induced serotonin release. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 1817-1821. PMID: 1347426, PMCID: PMC48544, DOI: 10.1073/pnas.89.5.1817.Peer-Reviewed Original ResearchConceptsPlasma membrane vesiclesMembrane vesiclesAmine transportersVesicular amine transporterBiogenic amine transportersSecretory vesiclesPlasma membraneATP hydrolysisMolecular mechanismsBovine adrenal chromaffin granulesSerotonin transporterAdrenal chromaffin granulesTransportersDirect interactionVesiclesChromaffin granulesHuman plateletsManner characteristicEffluxTransmembraneATPMDMA actionMechanismBindingMembraneExpression of a cloned gamma-aminobutyric acid transporter in mammalian cells.
Keynan S, Suh Y, Kanner B, Rudnick G. Expression of a cloned gamma-aminobutyric acid transporter in mammalian cells. Biochemistry 1992, 31: 1974-9. PMID: 1536839, DOI: 10.1021/bi00122a011.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCarrier ProteinsChloridesCloning, MolecularDNAGABA Plasma Membrane Transport ProteinsGamma-Aminobutyric AcidGene ExpressionGenetic VectorsHeLa CellsHumansKineticsL CellsMembrane ProteinsMembrane Transport ProteinsMiceNerve Tissue ProteinsOrganic Anion TransportersPlasmidsPrecipitin TestsRatsSodiumTransfectionTunicamycinXenopusConceptsMammalian cellsGABA transportMouse Ltk- cellsT7 RNA polymerasePlasma membrane vesiclesL cellsApparent molecular massGABA transporterSynaptic plasma membrane vesiclesGamma-aminobutyric acid transporterPresence of tunicamycinEukaryotic expression vectorRNA polymeraseTransient expressionExpression vectorAcid transportersMembrane vesiclesStable expressionLtk- cellsFunctional expressionGAT-1Molecular massHeLa cellsTransportersTransfection
1982
Coupling of transmembrane proton gradients to platelet serotonin transport.
Keyes S, Rudnick G. Coupling of transmembrane proton gradients to platelet serotonin transport. Journal Of Biological Chemistry 1982, 257: 1172-1176. PMID: 7056713, DOI: 10.1016/s0021-9258(19)68170-6.Peer-Reviewed Original ResearchConceptsPlasma membrane vesiclesSerotonin transportTransmembrane proton gradientPlatelet plasma membraneTransmembrane ion gradientsApparent competitionPlasma membraneMembrane vesiclesProton gradientIon gradientsSerotonin accumulationSole driving forceVesiclesAccumulationSerotonin transporterPH differencePhysiological pHTransportersTransportPH stimulationMembraneGradient
1979
Mechanism of imipramine inhibition of platelet 5-hydroxytryptamine transport.
Talvenheimo J, Nelson P, Rudnick G. Mechanism of imipramine inhibition of platelet 5-hydroxytryptamine transport. Journal Of Biological Chemistry 1979, 254: 4631-4635. PMID: 438209, DOI: 10.1016/s0021-9258(17)30057-1.Peer-Reviewed Original Research
1978
Reconstitution of 5-hydroxytryptamine transport from cholate-disrupted platelet plasma membrane vesicles.
Rudnick G, Nelson P. Reconstitution of 5-hydroxytryptamine transport from cholate-disrupted platelet plasma membrane vesicles. Biochemistry 1978, 17: 5300-3. PMID: 728400, DOI: 10.1021/bi00617a033.Peer-Reviewed Original ResearchConceptsPlatelet plasma membrane vesiclesPlasma membrane vesiclesMembrane vesiclesTransport activityNative membrane vesiclesHigh molecular weight aggregatesInhibitor sensitivityVesicular structuresVesiclesIntact plateletsWeight aggregatesIonic requirementsSoybean phospholipidsProteoliposomesTransportersActivityPhospholipidsReconstitutionPlatelet 5-hydroxytryptamine transport, an electroneutral mechanism coupled to potassium.
Rudnick G, Nelson P. Platelet 5-hydroxytryptamine transport, an electroneutral mechanism coupled to potassium. Biochemistry 1978, 17: 4739-42. PMID: 728383, DOI: 10.1021/bi00615a021.Peer-Reviewed Original ResearchConceptsPlasma membrane vesiclesLipophilic cation triphenylmethylphosphoniumMembrane potentialMembrane vesiclesElectroneutral mechanismVesicle membraneIon effluxPorcine blood plateletsAbsence of valinomycinAddition of valinomycinPotassium gradientBlood plateletsValinomycinElectrogenic mechanismMembraneNet influxMechanismDinitrophenolVesiclesTransportLittle effectInfluxTriphenylmethylphosphoniumAbsenceEfflux