Acidification of macrophage and fibroblast endocytic vesicles in vitro.
Galloway C, Dean G, Marsh M, Rudnick G, Mellman I. Acidification of macrophage and fibroblast endocytic vesicles in vitro. Proceedings Of The National Academy Of Sciences Of The United States Of America 1983, 80: 3334-3338. PMID: 6190176, PMCID: PMC394037, DOI: 10.1073/pnas.80.11.3334.Peer-Reviewed Original ResearchConceptsAcidic prelysosomal compartmentEndosome-lysosome fusionATP-dependent acidificationF0-ATPaseEndocytic vesiclesPrelysosomal compartmentAddition of ATPPrelysosomal vacuolesMitochondrial F1LysosomesEndosomesSecretory granulesCultured macrophagesN-ethylmaleimideInternal pHPercoll density gradientsAcidificationPH gradientFibroblastsCompartmentsOrganellesInhibitorsPermeant anionsFluorescein-dextranVacuoles