2012
Aggravation of inflammatory response by costimulation with titanium particles and mechanical perturbations in osteoblast- and macrophage-like cells
Lee HG, Hsu A, Goto H, Nizami S, Lee JH, Cadet ER, Tang P, Shaji R, Chandhanayinyong C, Kweon SH, Oh DS, Tawfeek H, Lee FY. Aggravation of inflammatory response by costimulation with titanium particles and mechanical perturbations in osteoblast- and macrophage-like cells. American Journal Of Physiology - Cell Physiology 2012, 304: c431-c439. PMID: 23255578, PMCID: PMC3602649, DOI: 10.1152/ajpcell.00202.2012.Peer-Reviewed Original ResearchConceptsBone-implant junctionEffect of particlesAbsence of TiWear particlesShear stressMechanical loadingTitanium particlesMetal implantsMechanical perturbationsMechanical strainFluid pressureParticlesBone tissueOsseous integrationTiOsteoprogenitor cellsImplant failureLoadingGene expressionCombined effectMechanical stimuliInterface
2011
Actin and ERK1/2-CEBPβ signaling mediates phagocytosis-induced innate immune response of osteoprogenitor cells
Lee HG, Minematsu H, Kim KO, Aydemir A, Shin MJ, Nizami SA, Chung KJ, Hsu AC, Jacobs CR, Lee FY. Actin and ERK1/2-CEBPβ signaling mediates phagocytosis-induced innate immune response of osteoprogenitor cells. Biomaterials 2011, 32: 9197-9206. PMID: 21899882, PMCID: PMC3193180, DOI: 10.1016/j.biomaterials.2011.08.059.Peer-Reviewed Original ResearchMeSH KeywordsActinsAdhesivenessAnimalsBenzimidazolesBone and BonesCCAAT-Enhancer-Binding Protein-betaCyclooxygenase 2Cytochalasin DExtracellular Signal-Regulated MAP KinasesGene Expression RegulationHumansImmunity, InnateInflammationInterleukin-6MAP Kinase Signaling SystemMiceModels, BiologicalOsteogenesisOsteolysisPhagocytosisProtein Kinase InhibitorsSignal TransductionSkullStem CellsTime FactorsTitaniumConceptsInflammatory osteolysisInflammatory responseMacrophage-mediated inflammatory responsesKey inflammatory pathwaysSuitable therapeutic targetInflammatory gene expressionInnate immune responseOsteoprogenitor cellsInflammatory cascadeInflammatory pathwaysImmune responseTherapeutic targetHost bone-implant interfaceAZD6244 treatmentIntracellular mechanismsBone-implant interfaceBone formationCellular mechanismsERK pathwayInflammationΒ pathwayOsteoclastogenesisOsteolysisIntracellular signalingImplant osteointegration
2007
Orthopedic Implant Particle‐Induced Tumor Necrosis Factor‐α Production in Macrophage–Monocyte Lineage Cells Is Mediated by Nuclear Factor of Activated T Cells
MINEMATSU H, SHIN MJ, AYDEMIR A, SEO SW, KIM DW, BLAINE TA, MACIÁN F, YANG J, LEE F. Orthopedic Implant Particle‐Induced Tumor Necrosis Factor‐α Production in Macrophage–Monocyte Lineage Cells Is Mediated by Nuclear Factor of Activated T Cells. Annals Of The New York Academy Of Sciences 2007, 1117: 143-150. PMID: 18056040, DOI: 10.1196/annals.1402.026.Peer-Reviewed Original ResearchConceptsActivated T cellsT cellsLineage cellsTumor Necrosis Factor-α ProductionNuclear factorMonocyte-macrophage lineage cellsTNF-alpha protein secretionTNF-alpha expressionTNF-alpha productionTNF-alpha gene expressionTumor necrosis factorMurine macrophage-like RAW264.7Macrophage-like RAW264.7TNF-alpha inductionMonocyte-macrophage cellsTNF-alphaNecrosis factorSpecific molecular mechanismsImplant particlesJoint prosthesesMolecular mechanismsERK Signaling Regulates Macrophage Colony‐Stimulating Factor Expression Induced by Titanium Particles in MC3T3.E1 Murine Calvarial Preosteoblastic Cells
SEO SW, LEE D, CHO SK, KIM AD, MINEMATSU H, AYDEMIR A, GELLER JA, MACAULAY W, YANG J, LEE F. ERK Signaling Regulates Macrophage Colony‐Stimulating Factor Expression Induced by Titanium Particles in MC3T3.E1 Murine Calvarial Preosteoblastic Cells. Annals Of The New York Academy Of Sciences 2007, 1117: 151-158. PMID: 18056041, DOI: 10.1196/annals.1402.027.Peer-Reviewed Original ResearchConceptsM-CSF expressionExtracellular signal-regulated kinase (ERK) pathwaySignal-regulated kinase pathwayPreosteoblastic cellsSignal transduction pathwaysSpecific ERK inhibitorReverse transcriptase-polymerase chain reactionEnzyme-linked immunosorbent assayPreosteoclast proliferationTransduction pathwaysKinase pathwayMolecular mechanismsMacrophage colony-stimulating factorCellular environmentERK inhibitorPeriprosthetic osteolysisM-CSF productionNuclear factor kappa beta ligandCell populationsBeta ligandSignificant clinical problemTranscriptase-polymerase chain reactionExpressionDose-dependent mannerColony-stimulating factor