1987
Refolding of bacteriorhodopsin in lipid bilayers A thermodynamically controlled two-stage process
Popot J, Gerchman S, Engelman D. Refolding of bacteriorhodopsin in lipid bilayers A thermodynamically controlled two-stage process. Journal Of Molecular Biology 1987, 198: 655-676. PMID: 3430624, DOI: 10.1016/0022-2836(87)90208-7.Peer-Reviewed Original ResearchConceptsLipid vesiclesAbsence of retinalAlpha-helical structureStable transmembrane helixPurple membrane latticeTransmembrane helicesSmall lipid vesiclesCircular dichroism spectraMembrane proteinsMixture of monomersFree energy minimumDodecyl sulfate solutionVesicle fusionRenatured moleculesSame absorption spectrumCorrect refoldingMajor rearrangementsStructure of bacteriorhodopsinTertiary structureMembrane latticeAbsorption spectroscopyNeutron crystallographyFolding mechanismPartial dehydration processLipid bilayers
1986
Reformation of crystalline purple membrane from purified bacteriorhodopsin fragments.
Popot J, Trewhella J, Engelman D. Reformation of crystalline purple membrane from purified bacteriorhodopsin fragments. The EMBO Journal 1986, 5: 3039-3044. PMID: 3792305, PMCID: PMC1167258, DOI: 10.1002/j.1460-2075.1986.tb04603.x.Peer-Reviewed Original Research