1991
The Escherichia coli hemL gene encodes glutamate 1-semialdehyde aminotransferase
Ilag L, Jahn D, Eggertsson G, Söll D. The Escherichia coli hemL gene encodes glutamate 1-semialdehyde aminotransferase. Journal Of Bacteriology 1991, 173: 3408-3413. PMID: 2045363, PMCID: PMC207952, DOI: 10.1128/jb.173.11.3408-3413.1991.Peer-Reviewed Original ResearchMeSH KeywordsAminolevulinic AcidCentrifugation, Density GradientChromatography, High Pressure LiquidCloning, MolecularDose-Response Relationship, DrugElectrophoresis, Polyacrylamide GelEscherichia coliIntramolecular TransferasesIsomerasesMolecular WeightPyridoxal PhosphatePyridoxamineTransformation, GeneticConceptsGlu-tRNA reductaseTRNA-dependent transformationApparent native molecular massMolecular massGlutamyl-tRNA synthetaseNative molecular massAminoglycoside antibiotic kanamycinHemL geneWild-type DNAAuxotrophic phenotypeC5 pathwaySodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisMap positionGSA aminotransferasePhysical mappingSulfate-polyacrylamide gel electrophoresisRate zonal sedimentationGene productsThird enzymeGlycerol gradientsApparent homogeneityAntibiotic kanamycinEscherichia coliPure proteinTwo glutamyl-tRNA reductase activities in Escherichia coli
Jahn D, Michelsen U, Söll D. Two glutamyl-tRNA reductase activities in Escherichia coli. Journal Of Biological Chemistry 1991, 266: 2542-2548. PMID: 1990004, DOI: 10.1016/s0021-9258(18)52279-1.Peer-Reviewed Original ResearchConceptsReductase activityGlu-tRNA reductaseMolecular massEscherichia coliApparent molecular massDifferent chromatographic separationsSequence-specific recognitionGlycerol gradient centrifugationThree-step conversionTetrapyrrole biosynthesisChlamydomonas reinhardtiiE. coli K12ALA formationChromatographic separationKey enzymeMonomeric structureActive enzymeBacillus subtilisColi K12Nondenaturing conditionsHomogeneous proteinMolecular weightDelta-aminolevulinic acidEnzyme activityAddition of GTP
1974
The phenylalanine tRNA from Mycoplasma sp. (Kid): a tRNA lacking hypermodified nucleosides functional in protein synthesis.
Kimball M, Soll D. The phenylalanine tRNA from Mycoplasma sp. (Kid): a tRNA lacking hypermodified nucleosides functional in protein synthesis. Nucleic Acids Research 1974, 1: 1713-20. PMID: 4615304, PMCID: PMC343450.Peer-Reviewed Original ResearchMeSH KeywordsAdenosineAlkenesAmino Acyl-tRNA SynthetasesCentrifugation, Density GradientEscherichia coliMycoplasmaNucleic Acid DenaturationPhenylalanineProtein BiosynthesisRNA, BacterialRNA, Transfer
1972
Glutamyl Transfer Ribonucleic Acid Synthetase of Escherichia coli II. INTERACTION WITH INTACT GLUTAMYL TRANSFER RIBONUCLEIC ACID
Lapointe J, Söll D. Glutamyl Transfer Ribonucleic Acid Synthetase of Escherichia coli II. INTERACTION WITH INTACT GLUTAMYL TRANSFER RIBONUCLEIC ACID. Journal Of Biological Chemistry 1972, 247: 4975-4981. PMID: 4341532, DOI: 10.1016/s0021-9258(19)44926-0.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmino Acyl-tRNA SynthetasesCarbon IsotopesCatalysisCentrifugation, Density GradientDiphosphatesDrug StabilityEscherichia coliGlutamatesHot TemperatureHydrogen-Ion ConcentrationKineticsLeucineMagnesiumPhosphorus IsotopesProtein BindingRNA, TransferSpectrometry, FluorescenceValineConceptsGlutamyl-transfer ribonucleic acid synthetaseEscherichia coli IITransfer ribonucleic acidTRNA-GluTRNA-ValTRNA-LeuCognate tRNABiological specificityRibonucleic acidPure enzymeEnzymeSimilar Km valuesComplex formationGradient centrifugationSynthetaseKm valuesFluorescence-quenching studiesTRNAIsoacceptorsComplexesFluorescence quenching studiesHeat inactivationInactivationLeuGluN6 - (Δ2 - Isopentenyl) Adenosine: Biosynthesis in vitro in transfer RNA by an enzyme purified from Eschericha coli
Bartz J, Söll D. N6 - (Δ2 - Isopentenyl) Adenosine: Biosynthesis in vitro in transfer RNA by an enzyme purified from Eschericha coli. Biochimie 1972, 54: 31-39. PMID: 4346747, DOI: 10.1016/s0300-9084(72)80035-x.Peer-Reviewed Original Research
1970
In Vitro Biosynthesis of Pseudouridine at the Polynucleotide Level by an Enzyme Extract from Escherichia coli
Johnson L, Söll D. In Vitro Biosynthesis of Pseudouridine at the Polynucleotide Level by an Enzyme Extract from Escherichia coli. Proceedings Of The National Academy Of Sciences Of The United States Of America 1970, 67: 943-950. PMID: 4943184, PMCID: PMC283296, DOI: 10.1073/pnas.67.2.943.Peer-Reviewed Original ResearchConceptsE. coli RNA polymeraseRNA transcription productsColi RNA polymeraseConversion of uridineE. coli extractsTRNA genesRNA polymerasePolynucleotide levelUridine residuesTranscription productsVitro BiosynthesisMycoplasma spEscherichia coliLambda DNARNADNAMacromolecular levelEnzyme extractBiosynthesisGenesPolymerasePseudouridineSpColiResiduesN6-(Δ2-isopentenyl)adenosine: Biosynthesis in vitro in transfer RNA by an enzyme purified from escherichia coli
Bartz J, Kline L, Söll D. N6-(Δ2-isopentenyl)adenosine: Biosynthesis in vitro in transfer RNA by an enzyme purified from escherichia coli. Biochemical And Biophysical Research Communications 1970, 40: 1481-1487. PMID: 4326583, DOI: 10.1016/0006-291x(70)90035-5.Peer-Reviewed Original ResearchMeSH KeywordsAdenineAdenine NucleotidesAlkenesAnimalsCentrifugation, Density GradientChromatography, PaperColiphagesEscherichia coliHydrogen-Ion ConcentrationHydrolysisLiverMolecular WeightMycoplasmaNucleosidesOrganophosphorus CompoundsPhosphoric AcidsPolynucleotidesPotassium PermanganateRatsRNA, BacterialRNA, TransferRNA, ViralTransferasesThe Interaction of Seryl and of Leucyl Transfer Ribonucleic Acid Synthetases with Their Cognate Transfer Ribonucleic Acids
Knowles J, Katze J, Konigsberg W, Söll D. The Interaction of Seryl and of Leucyl Transfer Ribonucleic Acid Synthetases with Their Cognate Transfer Ribonucleic Acids. Journal Of Biological Chemistry 1970, 245: 1407-1415. PMID: 4910800, DOI: 10.1016/s0021-9258(18)63251-x.Peer-Reviewed Original ResearchMeSH KeywordsAcrylatesAdenosine TriphosphateBuffersCentrifugation, Density GradientChromatography, GelDextransEscherichia coliGelsLeucineLigasesMethodsPhosphatesRNA, BacterialRNA, TransferSerineConceptsSeryl-tRNA synthetaseTransfer ribonucleic acidComplex formationTransfer RNA speciesLeucyl-tRNA synthetaseRibonucleic acidRNA speciesCognate tRNAEscherichia coliSynthetaseDensity gradient centrifugationTRNAStable complexesHigh saltGradient centrifugationSpeciesGel filtrationComplexesSerylColiATPEnzymeAcidSerFormation