1990
Inaccuracy and the Recognition of †RNA
Rogers M, Soll D. Inaccuracy and the Recognition of †RNA. Progress In Nucleic Acid Research And Molecular Biology 1990, 39: 185-208. PMID: 2247608, DOI: 10.1016/s0079-6603(08)60627-3.Peer-Reviewed Original ResearchConceptsATP-dependent stepNoncognate aminoacyl-tRNAsGlutaminyl-tRNA synthetaseAminoacyl-tRNA synthetasesRecognition of tRNAAmber mutationGlnRAminoacyl-tRNAEditing mechanismTRNAMutantsMischargingCentral roleEnzymeSynthetasesMisaminoacylationSupF.SupFSynthetaseMutationsGlutamineMechanismSuppressionAssays
1988
Site-directed mutagenesis to fine-tune enzyme specificity
Uemura H, Rogers M, Swanson R, Watson L, Söll D. Site-directed mutagenesis to fine-tune enzyme specificity. Protein Engineering Design And Selection 1988, 2: 293-296. PMID: 3150543, DOI: 10.1093/protein/2.4.293.Peer-Reviewed Original ResearchConceptsOligonucleotide-directed mutagenesisEscherichia coli glutaminyl-tRNA synthetaseGenetic selectionGlutaminyl-tRNA synthetaseAmino acid replacementsSite-directed mutagenesisAcid replacementsEnzyme specificitySingle residueMutagenesisSide chainsRepulsive charge-charge interactionsSpecific recognitionCharge-charge interactionsNucleic acidsMutantsProteinSupFSynthetaseResiduesGlutamineSelection