1983
Each element of the Drosophila tRNA Arg gene split promoter directs transcription in Xenopus oocytes
Sharp S, Dingermann T, Schaack J, Sharp J, Burke D, DeRobertis E, Söll D. Each element of the Drosophila tRNA Arg gene split promoter directs transcription in Xenopus oocytes. Nucleic Acids Research 1983, 11: 8677-8690. PMID: 6561520, PMCID: PMC326616, DOI: 10.1093/nar/11.24.8677.Peer-Reviewed Original ResearchConceptsD-control regionDrosophila tRNAArg geneTRNAArg geneEukaryotic tRNA gene transcriptionXenopus oocytesRNA polymerase III transcription factorT-control regionEukaryotic tRNA genesTRNA gene transcriptionIntragenic control regionTranscription initiation siteSpecific DNA sequencesTRNA genesTranscription initiationArg genesControl regionTranscription factorsGene transcriptionDNA sequencesMutant formsOocyte nucleusSequence 5Initiation siteGenesTranscriptionStable transcription complex formation of eukaryotic tRNA genes is dependent on a limited separation of the two intragenic control regions.
Dingermann T, Sharp S, Schaack J, Söll D. Stable transcription complex formation of eukaryotic tRNA genes is dependent on a limited separation of the two intragenic control regions. Journal Of Biological Chemistry 1983, 258: 10395-10402. PMID: 6309803, DOI: 10.1016/s0021-9258(17)44470-x.Peer-Reviewed Original ResearchConceptsIntragenic control regionControl regionTRNA genesTRNAArg geneDrosophila Kc cell extractStable transcription complex formationTranscription efficiencyDrosophila tRNAArg geneT-control regionMutant tRNA genesEukaryotic tRNA genesTranscription complex formationStable transcription complexesWild-type geneXhoI linkerTranscriptional roleTranscription initiationTranscription complexDNA regionsType genesTranscription factorsGene transcriptionTermination sitesDNA fragmentsCell extractsTranscription of eukaryotic tRNA genes in vitro. I. Analysis of control regions using a competition assay.
Sharp S, Dingermann T, Schaack J, DeFranco D, Söll D. Transcription of eukaryotic tRNA genes in vitro. I. Analysis of control regions using a competition assay. Journal Of Biological Chemistry 1983, 258: 2440-2446. PMID: 6549757, DOI: 10.1016/s0021-9258(18)32945-4.Peer-Reviewed Original ResearchConceptsT-control regionD-control regionTRNA gene transcriptionTRNAArg geneControl regionTranscription factorsCompetitive abilityDeletion mutantsGene transcriptionEukaryotic tRNA gene transcriptionDrosophila tRNAArg geneEukaryotic tRNA genesStem regionIntragenic control regionEfficiency of transcriptionTRNA genesTranscription extractTRNA productD-loopTranscription levelsWild typeTranscriptionT-loopGenesCompetition assays
1982
The minimum intragenic sequences required for promotion of eukaryotic tRNA gene transcription
Sharp S, Dingermann T, Söll D. The minimum intragenic sequences required for promotion of eukaryotic tRNA gene transcription. Nucleic Acids Research 1982, 10: 5393-5406. PMID: 6924209, PMCID: PMC320884, DOI: 10.1093/nar/10.18.5393.Peer-Reviewed Original ResearchConceptsD-control regionTRNA genesIntragenic control regionTranscription systemControl regionTRNAArg geneRNA synthesisGene transcriptionEukaryotic tRNA gene transcriptionHeLa cell transcription systemDrosophila tRNAArg geneT-control regionMutant tRNA genesEukaryotic tRNA genesTRNA gene transcriptionRNA polymerase IIIWild-type geneAdjacent flanking sequencesHeLa systemsMature tRNAIntragenic regionsSpecific transcriptionPolymerase IIIXenopus laevis oocytesDNA sequences