1984
The extent of a eukaryotic tRNA gene. 5‘- and 3‘-flanking sequence dependence for transcription and stable complex formation.
Schaack J, Sharp S, Dingermann T, Burke DJ, Cooley L, Söll D. The extent of a eukaryotic tRNA gene. 5‘- and 3‘-flanking sequence dependence for transcription and stable complex formation. Journal Of Biological Chemistry 1984, 259: 1461-1467. PMID: 6693417, DOI: 10.1016/s0021-9258(17)43429-6.Peer-Reviewed Original ResearchConceptsStable complex formationBase pairsDrosophila Kc cell extractSequence requirementsCell extractsEukaryotic tRNA genesStable transcription complexesHeLa cell extractsTRNA genesComplex formationTranscription complexArg genesEfficient transcriptionTranscription assaysTranscription propertiesCell-free extractsTranscriptionHomologous systemGenesSequenceSequence dependenceCellular sourceExtractAssaysPairs
1983
Each element of the Drosophila tRNA Arg gene split promoter directs transcription in Xenopus oocytes
Sharp S, Dingermann T, Schaack J, Sharp J, Burke D, DeRobertis E, Söll D. Each element of the Drosophila tRNA Arg gene split promoter directs transcription in Xenopus oocytes. Nucleic Acids Research 1983, 11: 8677-8690. PMID: 6561520, PMCID: PMC326616, DOI: 10.1093/nar/11.24.8677.Peer-Reviewed Original ResearchConceptsD-control regionDrosophila tRNAArg geneTRNAArg geneEukaryotic tRNA gene transcriptionXenopus oocytesRNA polymerase III transcription factorT-control regionEukaryotic tRNA genesTRNA gene transcriptionIntragenic control regionTranscription initiation siteSpecific DNA sequencesTRNA genesTranscription initiationArg genesControl regionTranscription factorsGene transcriptionDNA sequencesMutant formsOocyte nucleusSequence 5Initiation siteGenesTranscriptionStable transcription complex formation of eukaryotic tRNA genes is dependent on a limited separation of the two intragenic control regions.
Dingermann T, Sharp S, Schaack J, Söll D. Stable transcription complex formation of eukaryotic tRNA genes is dependent on a limited separation of the two intragenic control regions. Journal Of Biological Chemistry 1983, 258: 10395-10402. PMID: 6309803, DOI: 10.1016/s0021-9258(17)44470-x.Peer-Reviewed Original ResearchConceptsIntragenic control regionControl regionTRNA genesTRNAArg geneDrosophila Kc cell extractStable transcription complex formationTranscription efficiencyDrosophila tRNAArg geneT-control regionMutant tRNA genesEukaryotic tRNA genesTranscription complex formationStable transcription complexesWild-type geneXhoI linkerTranscriptional roleTranscription initiationTranscription complexDNA regionsType genesTranscription factorsGene transcriptionTermination sitesDNA fragmentsCell extractsTranscription of eukaryotic tRNA genes in vitro. I. Analysis of control regions using a competition assay.
Sharp S, Dingermann T, Schaack J, DeFranco D, Söll D. Transcription of eukaryotic tRNA genes in vitro. I. Analysis of control regions using a competition assay. Journal Of Biological Chemistry 1983, 258: 2440-2446. PMID: 6549757, DOI: 10.1016/s0021-9258(18)32945-4.Peer-Reviewed Original ResearchConceptsT-control regionD-control regionTRNA gene transcriptionTRNAArg geneControl regionTranscription factorsCompetitive abilityDeletion mutantsGene transcriptionEukaryotic tRNA gene transcriptionDrosophila tRNAArg geneEukaryotic tRNA genesStem regionIntragenic control regionEfficiency of transcriptionTRNA genesTranscription extractTRNA productD-loopTranscription levelsWild typeTranscriptionT-loopGenesCompetition assaysTranscription of eukaryotic tRNA genes in vitro. II. Formation of stable complexes.
Schaack J, Sharp S, Dingermann T, Söll D. Transcription of eukaryotic tRNA genes in vitro. II. Formation of stable complexes. Journal Of Biological Chemistry 1983, 258: 2447-2453. PMID: 6549758, DOI: 10.1016/s0021-9258(18)32946-6.Peer-Reviewed Original ResearchConceptsStable transcription complex formationTRNA genesTranscription complex formationStable transcription complexesTranscription complexDrosophila Kc cell extractGene regionD-stemDrosophila tRNAArg geneEukaryotic tRNA genesDrosophila tRNA genesTranscription termination sequenceTRNAArg geneStable complexesComplex formationTranscription experimentsDNA regionsTranscription factorsFactor bindingCell-free extractsTermination sequenceSequence 5T-stemCell extractsDeletion mutations
1982
The minimum intragenic sequences required for promotion of eukaryotic tRNA gene transcription
Sharp S, Dingermann T, Söll D. The minimum intragenic sequences required for promotion of eukaryotic tRNA gene transcription. Nucleic Acids Research 1982, 10: 5393-5406. PMID: 6924209, PMCID: PMC320884, DOI: 10.1093/nar/10.18.5393.Peer-Reviewed Original ResearchConceptsD-control regionTRNA genesIntragenic control regionTranscription systemControl regionTRNAArg geneRNA synthesisGene transcriptionEukaryotic tRNA gene transcriptionHeLa cell transcription systemDrosophila tRNAArg geneT-control regionMutant tRNA genesEukaryotic tRNA genesTRNA gene transcriptionRNA polymerase IIIWild-type geneAdjacent flanking sequencesHeLa systemsMature tRNAIntragenic regionsSpecific transcriptionPolymerase IIIXenopus laevis oocytesDNA sequencesEukaryotic tRNA gene transcription is controlled by signals within and outside the mature coding sequence.
DeFranco D, Dingermann T, Johnson D, Sharp S, Söll D. Eukaryotic tRNA gene transcription is controlled by signals within and outside the mature coding sequence. Princess Takamatsu Symposia 1982, 12: 63-72. PMID: 7166550.Peer-Reviewed Original ResearchConceptsTRNA genesMature coding sequenceTranscriptional repressionTRNA sequencesControl regionCoding sequenceEukaryotic tRNA gene transcriptionDrosophila tRNAArg geneSecond control regionEukaryotic tRNA genesDrosophila tRNA genesTRNA gene transcriptionMature tRNA sequencesInitiation of transcriptionNuclease Bal 31TRNAArg geneMature tRNAPoor transcriptional activityDeletion analysisGene transcriptionNucleotides 8D-loopTranscriptional activityPromoter regionBAL-31
1980
IN VITRO TRANSCRIPTION OF CLONED EUKARYOTIC tRNA GENES
Schmidt O, Hovemann B, Silverman S, Yamada H, Mao J, Söll D. IN VITRO TRANSCRIPTION OF CLONED EUKARYOTIC tRNA GENES. 1980, 179-188. DOI: 10.1016/b978-0-08-024417-4.50021-4.Peer-Reviewed Original ResearchTRNA genesTRNA precursorsEukaryotic tRNA genesYeast tRNA genesRegulatory DNA regionsRNA polymerase IIICorresponding RNA sequencesXenopus germinal vesicleXenopus nuclear extractsPrecursor tRNAsMature tRNADNA regionsPrimary transcriptTRNA biosynthesisPolymerase IIIGene transcriptionDNA sequencesNuclear extractsRNA sequencesSequence analysisTRNAGerminal vesicleGenesTranscriptionSequence
1978
Specific transcription of eukaryotic tRNA genes in Xenopus germinal vesicle extracts.
Schmidt O, Mao J, Silverman S, Hovemann B, Söll D. Specific transcription of eukaryotic tRNA genes in Xenopus germinal vesicle extracts. Proceedings Of The National Academy Of Sciences Of The United States Of America 1978, 75: 4819-4823. PMID: 105357, PMCID: PMC336212, DOI: 10.1073/pnas.75.10.4819.Peer-Reviewed Original ResearchConceptsGerminal vesicle extractsTRNA genesRNA speciesVesicle extractsEukaryotic gene transcriptionEukaryotic tRNA genesRegulatory DNA regionsPrecursor tRNA moleculesXenopus germinal vesiclePrecursor tRNAsSpecific transcriptionDNA regionsTRNA biosynthesisTRNA precursorsNucleotide modificationsGene transcriptionTRNA moleculesTranscription systemNucleotide analysisTRNAGerminal vesicleGenesTranscriptionTRNALysSpecies