1997
Two Basic Amino Acids in the Second Inner Loop of the Interleukin-8 Receptor Are Essential for Gα16 Coupling*
Xie W, Jiang H, Wu Y, Wu D. Two Basic Amino Acids in the Second Inner Loop of the Interleukin-8 Receptor Are Essential for Gα16 Coupling*. Journal Of Biological Chemistry 1997, 272: 24948-24951. PMID: 9312098, DOI: 10.1074/jbc.272.40.24948.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigens, CDArginineBinding SitesCOS CellsGTP-Binding ProteinsInterleukin-8KineticsLysineMolecular Sequence DataMutagenesis, Site-DirectedPhosphatidylinositol Diacylglycerol-LyaseProtein Structure, SecondaryReceptors, InterleukinReceptors, Interleukin-8ARecombinant Fusion ProteinsTransfectionType C Phospholipases
1993
Identification of critical regions on phospholipase C-beta 1 required for activation by G-proteins.
Wu D, Jiang H, Katz A, Simon M. Identification of critical regions on phospholipase C-beta 1 required for activation by G-proteins. Journal Of Biological Chemistry 1993, 268: 3704-3709. PMID: 8381437, DOI: 10.1016/s0021-9258(18)53750-9.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCarbacholCell LineCloning, MolecularEnzyme ActivationGTP-Binding ProteinsInositol PhosphatesKineticsMolecular Sequence DataMutagenesisNorepinephrinePhosphatidylinositol Diacylglycerol-LyasePhosphoric Diester HydrolasesPlasmidsReceptors, MuscarinicRecombinant ProteinsRestriction MappingSequence DeletionTransfectionConceptsG alpha q.PLC-beta 1COS-7 cellsG alpha qAlpha q.Alpha subunitG protein alpha subunitsAlpha qMuscarinic acetylcholine receptor type 1G alpha subunitsPhosphoinositide-specific phospholipase CIntrinsic enzymatic activitySpecific deletionAmino acid sequenceLigand-induced releasePhospholipase C beta 1C beta 1CDNA clonesAcid sequenceC-terminusG proteinsTerminal endDependent activationPhospholipase CAlpha 1C-adrenergic receptor
1992
Activation of phospholipase C by the alpha subunits of the Gq and G11 proteins in transfected Cos-7 cells.
Wu D, Lee C, Rhee S, Simon M. Activation of phospholipase C by the alpha subunits of the Gq and G11 proteins in transfected Cos-7 cells. Journal Of Biological Chemistry 1992, 267: 1811-1817. PMID: 1309799, DOI: 10.1016/s0021-9258(18)46018-8.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodiesCell LineCell MembraneEnzyme ActivationGTP-Binding ProteinsGuanosine 5'-O-(3-Thiotriphosphate)InositolInositol PhosphatesIsoenzymesKineticsMacromolecular SubstancesMolecular Sequence DataMutagenesis, Site-DirectedPeptidesPhosphatidylinositol Diacylglycerol-LyasePhosphoinositide Phospholipase CPhosphoric Diester HydrolasesTransfectionType C PhospholipasesConceptsG alpha 11G alpha qCos-7 cellsG alpha zAlpha qPI-PLCAlpha 11Alpha subunitAlpha zG protein alpha subunitsProtein alpha subunitsAlpha-subunit cDNAHigh steady-state levelsPhospholipase C beta 1Introduction of mutationsCell-free systemHigh-efficiency transient transfectionG protein activationC beta 1Glutamine 205Steady-state levelsBeta 1Transient transfectionProtein activationLeucine mutant
1991
Antibodies to the alpha q subfamily of guanine nucleotide-binding regulatory protein alpha subunits attenuate activation of phosphatidylinositol 4,5-bisphosphate hydrolysis by hormones.
Gutowski S, Smrcka A, Nowak L, Wu D, Simon M, Sternweis P. Antibodies to the alpha q subfamily of guanine nucleotide-binding regulatory protein alpha subunits attenuate activation of phosphatidylinositol 4,5-bisphosphate hydrolysis by hormones. Journal Of Biological Chemistry 1991, 266: 20519-20524. PMID: 1657928, DOI: 10.1016/s0021-9258(18)54955-3.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAnimalsBlotting, WesternBradykininCell LineElectrophoresis, Polyacrylamide GelGTP-Binding ProteinsHormonesHydrolysisImmune SeraPhosphatidylinositol 4,5-DiphosphatePhosphatidylinositol Diacylglycerol-LyasePhosphatidylinositol PhosphatesPhosphoric Diester HydrolasesPrecipitin TestsRatsConceptsAlpha qHormonal regulationNG108-15 cellsBisphosphate hydrolysisPhospholipase CRegulatory protein alphaPhospholipase C activityAlpha 11Attenuate activationG protein alpha subunitsAffinity-purified antibodiesProtein alphaProtein alpha subunitsAntibodiesGuanosine 5'Rat liverGq familyCommon carboxyl terminusC activityPhospholipase C.Alpha subunitDetergent extractsAntiserumActivationPeptides