2020
Sequence-dependent dynamics of synthetic and endogenous RSSs in V(D)J recombination
Hirokawa S, Chure G, Belliveau NM, Lovely GA, Anaya M, Schatz DG, Baltimore D, Phillips R. Sequence-dependent dynamics of synthetic and endogenous RSSs in V(D)J recombination. Nucleic Acids Research 2020, 48: gkaa418-. PMID: 32449932, PMCID: PMC7337519, DOI: 10.1093/nar/gkaa418.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDNA CleavageLymphocytesNucleic Acid ConformationProtein Sorting SignalsReceptors, AntigenSingle Molecule ImagingV(D)J RecombinationVertebrates
2007
Fluorescence Resonance Energy Transfer Analysis of Recombination Signal Sequence Configuration in the RAG1/2 Synaptic Complex
Ciubotaru M, Kriatchko AN, Swanson PC, Bright FV, Schatz DG. Fluorescence Resonance Energy Transfer Analysis of Recombination Signal Sequence Configuration in the RAG1/2 Synaptic Complex. Molecular And Cellular Biology 2007, 27: 4745-4758. PMID: 17470556, PMCID: PMC1951485, DOI: 10.1128/mcb.00177-07.Peer-Reviewed Original Research
2006
Mobilization of RAG-Generated Signal Ends by Transposition and Insertion In Vivo
Chatterji M, Tsai CL, Schatz DG. Mobilization of RAG-Generated Signal Ends by Transposition and Insertion In Vivo. Molecular And Cellular Biology 2006, 26: 1558-1568. PMID: 16449665, PMCID: PMC1367191, DOI: 10.1128/mcb.26.4.1558-1568.2006.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCell LineDNADNA-Binding ProteinsHomeodomain ProteinsHumansNuclear ProteinsPlasmidsProtein Sorting SignalsRecombinant ProteinsSequence DeletionTransposasesConceptsRAG proteinsVertebrate cellsTransposition eventsEnd fragmentsFull-length RAG2Embryonic kidney cell lineHuman embryonic kidney cell lineTarget site duplicationsGenome instabilityHuman genomeSignal endsKidney cell lineGenomic instabilityTranslocation eventsSite duplicationsChromosomal translocationsDNA cleavageComplex rearrangementsChromosome deletionsEssential roleProteinCell linesEpisomesDeletionAssays
2003
A Functional Analysis of the Spacer of V(D)J Recombination Signal Sequences
Lee AI, Fugmann SD, Cowell LG, Ptaszek LM, Kelsoe G, Schatz DG. A Functional Analysis of the Spacer of V(D)J Recombination Signal Sequences. PLOS Biology 2003, 1: e1. PMID: 14551903, PMCID: PMC212687, DOI: 10.1371/journal.pbio.0000001.Peer-Reviewed Original ResearchConceptsRecombination signal sequencesSignal sequenceGene segmentsProtein-DNA interactionsLevel of recombinationDegree of conservationParticular functional importanceJ gene segmentsAntigen receptor genesSpacer variantsRAG proteinsRecombination machineryRSS activityInactive pseudogeneRSS functionSpacer sequencesFunctional analysisInteraction surfaceFunctional importanceLymphocyte developmentNumerous complex interactionsBiochemical assaysDistinct cooperationReceptor geneHeptamer
2000
The RAG Proteins and V(D)J Recombination: Complexes, Ends, and Transposition
Fugmann S, Lee A, Shockett P, Villey I, Schatz D. The RAG Proteins and V(D)J Recombination: Complexes, Ends, and Transposition. Annual Review Of Immunology 2000, 18: 495-527. PMID: 10837067, DOI: 10.1146/annurev.immunol.18.1.495.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
1997
Identification of V(D)J recombination coding end intermediates in normal thymocytes 11Edited by K. Yamamoto
Livák F, Schatz D. Identification of V(D)J recombination coding end intermediates in normal thymocytes 11Edited by K. Yamamoto. Journal Of Molecular Biology 1997, 267: 1-9. PMID: 9096202, DOI: 10.1006/jmbi.1996.0834.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDNA RepairGene RearrangementImmunoglobulin Joining RegionImmunoglobulin Variable RegionMiceMice, SCIDProtein Sorting SignalsRecombination, GeneticThymus GlandConceptsRecombination signal sequencesNormal lymphoid precursorsSignal endsJ alpha genesPre-B cell linesGene rearrangement processDouble-strand breaksNormal murine thymocytesSignal sequenceLymphoid precursorsK. YamamotoAlpha geneFirst direct demonstrationHairpin structureLow abundanceStrand breaksGene segmentsCell linesAntigen receptorMurine thymocytesRecombinationDirect demonstrationVivoJoint formationNormal thymocytes