2021
Distinct roles of KLF4 in mesenchymal cell subtypes during lung fibrogenesis
Chandran RR, Xie Y, Gallardo-Vara E, Adams T, Garcia-Milian R, Kabir I, Sheikh AQ, Kaminski N, Martin KA, Herzog EL, Greif DM. Distinct roles of KLF4 in mesenchymal cell subtypes during lung fibrogenesis. Nature Communications 2021, 12: 7179. PMID: 34893592, PMCID: PMC8664937, DOI: 10.1038/s41467-021-27499-8.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell ProliferationDisease Models, AnimalDown-RegulationExtracellular MatrixFemaleFibroblastsFibrosisHumansKruppel-Like Factor 4LungLung InjuryMaleMesenchymal Stem CellsMiceMice, Inbred C57BLMyofibroblastsReceptor, Platelet-Derived Growth Factor betaRespiratory Tract DiseasesSignal TransductionTransforming Growth Factor betaConceptsMesenchymal cell typesPlatelet-derived growth factor receptorSmooth muscle actinLung fibrosisKruppel-like factor 4Forkhead box M1Growth factor receptorCell transitionCell typesExtracellular matrixDistinct rolesKLF4Box M1C chemokine ligandMesenchymal cell subtypesFactor receptorPro-fibrotic effectsFactor 4PDGFRMesenchymeCellsMacrophage accumulationKLF4 levelsChemokine ligandLung fibrogenesis
2020
Specialized Smooth Muscle Cell Progenitors in Pulmonary Hypertension
Saddouk F, Ntokou A, Greif D. Specialized Smooth Muscle Cell Progenitors in Pulmonary Hypertension. 2020, 25-30. DOI: 10.1007/978-981-15-1185-1_4.ChaptersSmooth muscle cellsPulmonary hypertensionNormal lungArteriole smooth muscle cellsPulmonary artery complianceSmooth muscle cell progenitorsStrong independent predictorPlatelet-derived growth factor receptorKruppel-like factor 4Diverse cardiovascular diseasesHypoxia-inducible factorGrowth factor receptorArtery complianceIndependent predictorsTherapeutic optionsIdiopathic formCardiovascular diseaseDependent signaling contributesSignaling contributesHypertensionSMC progenitorsMuscle cellsFactor receptorNon-cell autonomous mannerCell progenitors
2018
NCK-dependent pericyte migration promotes pathological neovascularization in ischemic retinopathy
Dubrac A, Künzel SE, Künzel SH, Li J, Chandran RR, Martin K, Greif DM, Adams RH, Eichmann A. NCK-dependent pericyte migration promotes pathological neovascularization in ischemic retinopathy. Nature Communications 2018, 9: 3463. PMID: 30150707, PMCID: PMC6110853, DOI: 10.1038/s41467-018-05926-7.Peer-Reviewed Original ResearchConceptsNeovascular tuftsPericyte migrationMural cellsPathological neovascular tuftsPDGF-B overexpressionTreatment of retinopathyOxygen-induced retinopathyCapillary barrier functionΑ-SMA expressionPlatelet-derived growth factor receptorPlatelet-derived growth factorPhosphorylation of PDGFRβGrowth factor receptorIschemic retinopathyProliferative retinopathyEndothelial-specific knockoutVascular leakagePericyte depletionPathological neovascularizationGenetic lineage tracingMouse modelPericyte proliferationNck2 adaptor proteinRetinopathyBarrier function
2015
Smooth muscle cell progenitors are primed to muscularize in pulmonary hypertension
Sheikh AQ, Misra A, Rosas IO, Adams RH, Greif DM. Smooth muscle cell progenitors are primed to muscularize in pulmonary hypertension. Science Translational Medicine 2015, 7: 308ra159. PMID: 26446956, PMCID: PMC4629985, DOI: 10.1126/scitranslmed.aaa9712.Peer-Reviewed Original ResearchConceptsSmooth muscle cellsKruppel-like factor 4Pulmonary hypertensionSmooth muscleHypoxia-induced pulmonary hypertensionPathogenesis of PHPulmonary artery blood pressureSMC progenitorsArteriole smooth muscleArtery blood pressureSmooth muscle cell progenitorsCardiovascular disease pathogenesisPlatelet-derived growth factor receptorHypoxia-induced expressionGrowth factor receptorPH patientsBlood pressurePulmonary arteriolesVascular disordersTherapeutic strategiesDisease pathogenesisKLF4 levelsKLF4 expressionDistal extensionMuscle cells