2022
Chapter 8 Out to the tissues the arterial side (arteries, arterioles—development, structure, functions, and pathologies)
Dave J, Saito J, Mottola G, Greif D. Chapter 8 Out to the tissues the arterial side (arteries, arterioles—development, structure, functions, and pathologies). 2022, 89-98. DOI: 10.1016/b978-0-12-822546-2.00015-0.Peer-Reviewed Original Research
2021
Distinct roles of KLF4 in mesenchymal cell subtypes during lung fibrogenesis
Chandran RR, Xie Y, Gallardo-Vara E, Adams T, Garcia-Milian R, Kabir I, Sheikh AQ, Kaminski N, Martin KA, Herzog EL, Greif DM. Distinct roles of KLF4 in mesenchymal cell subtypes during lung fibrogenesis. Nature Communications 2021, 12: 7179. PMID: 34893592, PMCID: PMC8664937, DOI: 10.1038/s41467-021-27499-8.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell ProliferationDisease Models, AnimalDown-RegulationExtracellular MatrixFemaleFibroblastsFibrosisHumansKruppel-Like Factor 4LungLung InjuryMaleMesenchymal Stem CellsMiceMice, Inbred C57BLMyofibroblastsReceptor, Platelet-Derived Growth Factor betaRespiratory Tract DiseasesSignal TransductionTransforming Growth Factor betaConceptsMesenchymal cell typesPlatelet-derived growth factor receptorSmooth muscle actinLung fibrosisKruppel-like factor 4Forkhead box M1Growth factor receptorCell transitionCell typesExtracellular matrixDistinct rolesKLF4Box M1C chemokine ligandMesenchymal cell subtypesFactor receptorPro-fibrotic effectsFactor 4PDGFRMesenchymeCellsMacrophage accumulationKLF4 levelsChemokine ligandLung fibrogenesis
2019
Promoters to Study Vascular Smooth Muscle
Chakraborty R, Saddouk FZ, Carrao AC, Krause DS, Greif DM, Martin KA. Promoters to Study Vascular Smooth Muscle. Arteriosclerosis Thrombosis And Vascular Biology 2019, 39: 603-612. PMID: 30727757, PMCID: PMC6527360, DOI: 10.1161/atvbaha.119.312449.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsCell LineCell LineageCell TransdifferentiationGene Expression RegulationGene Knockout TechniquesGene TargetingHumansMiceMicrofilament ProteinsMuscle ProteinsMuscle, Smooth, VascularMyocytes, Smooth MuscleMyofibroblastsMyosin Heavy ChainsNeovascularization, PathologicNeovascularization, PhysiologicPhenotypePromoter Regions, GeneticRecombinant Fusion ProteinsConceptsSmooth muscle cellsCre driver linesDiversity of phenotypesMuscle cell typesVisceral smooth muscle cellsSMC transdifferentiationActa2 promoterRemarkable plasticityExciting new eraSMC functionCell typesCre linesEmbryonic heartExciting discoveriesPhenotypeMuscle cellsPerivascular adipocytesPromoterVascular smooth muscleNonmuscular cellsExpressionMyeloid cellsCardiovascular phenotypesCellsBlood vessel wall
2017
Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta.
Misra A, Feng Z, Zhang J, Lou ZY, Greif DM. Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta. Journal Of Visualized Experiments 2017 PMID: 28930997, PMCID: PMC5752224, DOI: 10.3791/56039.Peer-Reviewed Original ResearchConceptsSmooth muscle cellsAortic smooth muscle cellsPregnant micePharmacological agentsAortic wallAortaLarge arteriesAdult aortaMuscle cellsEndothelial cellsPathological modelsHypothesis-generating experimentsContinuous exposureCell explantsTissue explantsPathogenesisFate mappingSpecific gene targetsClonal analysisNormal developmentVivoGene targetsExtracellular matrixClonal architectureCellsWhere do new endothelial cells come from in the injured heart?
Greif DM, Eichmann A. Where do new endothelial cells come from in the injured heart? Nature Reviews Cardiology 2017, 14: 507-508. PMID: 28770866, DOI: 10.1038/nrcardio.2017.121.Commentaries, Editorials and Letters
2016
Chapter Eight Vascular Cells in Blood Vessel Wall Development and Disease
Mazurek R, Dave JM, Chandran RR, Misra A, Sheikh AQ, Greif DM. Chapter Eight Vascular Cells in Blood Vessel Wall Development and Disease. Advances In Pharmacology 2016, 78: 323-350. PMID: 28212800, PMCID: PMC5559712, DOI: 10.1016/bs.apha.2016.08.001.BooksConceptsDistinct cellular layersVascular cellsRegulated processGenetic approachesWall developmentDevelopmental eventsIndividual cellsDiverse vascular diseasesCellular mechanismsCell proliferationCellsNormal vascular wallCellular layersMorphogenesisTherapeutic strategiesBiologyDifferentiationVascular wallMajor ramificationsProliferationFurther investigationVessel wallMigration
2013
Development and pathologies of the arterial wall
Seidelmann SB, Lighthouse JK, Greif DM. Development and pathologies of the arterial wall. Cellular And Molecular Life Sciences 2013, 71: 1977-1999. PMID: 24071897, PMCID: PMC11113178, DOI: 10.1007/s00018-013-1478-y.Peer-Reviewed Original ResearchMeSH KeywordsAngiogenic ProteinsAnimalsArteriesCardiovascular DiseasesCell DifferentiationCell LineageEndothelial CellsEndothelium, VascularGene Expression Regulation, DevelopmentalHumansMorphogenesisMuscle, Smooth, VascularMyocytes, Smooth MuscleNeovascularization, PathologicNeovascularization, PhysiologicConceptsAdventitial progenitor cellsDevastating vascular diseaseDevelopmental biologyWall developmentSmooth muscle cell originHuman diseasesExtracellular matrixMuscle cell originProcess of angiogenesisProgenitor cellsEndothelial networksDisease mechanismsNovel therapeutic strategiesDevelopmental studiesEndothelial cellsSmooth muscleCellsMorphogenesisTherapeutic strategiesCell originBiologyDifferentiationVascular diseaseVascular wallVascular wall abnormalities
2012
Radial Construction of an Arterial Wall
Greif DM, Kumar M, Lighthouse JK, Hum J, An A, Ding L, Red-Horse K, Espinoza FH, Olson L, Offermanns S, Krasnow MA. Radial Construction of an Arterial Wall. Developmental Cell 2012, 23: 482-493. PMID: 22975322, PMCID: PMC3500096, DOI: 10.1016/j.devcel.2012.07.009.Peer-Reviewed Original ResearchConceptsInner layer cellsCoordinated processGenetic analysisSuccessive cell layersVessel-specific differencesSignaling pathwaysCell reorientationSequential inductionEndothelial tubesSignal gradientMuscle cellsSignal contributesSerious diseaseCellsCell layerLayer cellsRadial migrationMesenchymeConcentric layersPathwayArterial wallPDGFInvasionInductionArtery wall