InSeq analysis of defined Legionella pneumophila libraries identifies a transporter-encoding gene cluster important for intracellular replication in mammalian hosts.
Moss C, Roy C. InSeq analysis of defined Legionella pneumophila libraries identifies a transporter-encoding gene cluster important for intracellular replication in mammalian hosts. MBio 2024, e0195524. PMID: 39365064, DOI: 10.1128/mbio.01955-24.Peer-Reviewed Original ResearchMammalian hostsLegionella pneumophila</i>Mutant libraryIntracellular replicationDot/Icm type IV secretion systemHost cellsType IV secretion systemInfection of mammalian cellsTransposon insertion sequencingTransposon mutant libraryOpportunistic human pathogenIV secretion systemIsogenic deletion mutantsGenetic complementation studiesHost-pathogen interactionsIntracellular bacterial pathogensMultidrug efflux pumpsMammalian infection modelsATP-binding cassetteBacteria encounterDot/Icm systemSecretion systemTransposon sequencesGene clusterHost cytosol