2024
Electrophysiology of fluoride channels in the yeasts Saccharomyces cerevisiae and Candida albicans
Rivetta A, Slayman C. Electrophysiology of fluoride channels in the yeasts Saccharomyces cerevisiae and Candida albicans. Methods In Enzymology 2024, 696: 3-24. PMID: 38658085, DOI: 10.1016/bs.mie.2024.01.005.Peer-Reviewed Original ResearchYeast Saccharomyces cerevisiaeOpportunistic human pathogenFree-living microorganismsSingle cellsPlasma membrane of single cellsYeast spheroplastsSaccharomyces cerevisiaeRegulation of moleculesCandida albicansHuman pathogensPlasma membraneCell wallExtracellular environmentMembrane of single cellsPatch-clamp techniqueOsmotic strengthYeastSpheroplastsCandidaFluoride channelsSmall cell volumeToxic moleculesCell membraneDiverse moleculesCell populations
2015
Yeast Fex1p Is a Constitutively Expressed Fluoride Channel with Functional Asymmetry of Its Two Homologous Domains*
Smith KD, Gordon PB, Rivetta A, Allen KE, Berbasova T, Slayman C, Strobel SA. Yeast Fex1p Is a Constitutively Expressed Fluoride Channel with Functional Asymmetry of Its Two Homologous Domains*. Journal Of Biological Chemistry 2015, 290: 19874-19887. PMID: 26055717, PMCID: PMC4528147, DOI: 10.1074/jbc.m115.651976.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceCell MembraneConserved SequenceDrug Resistance, FungalEscherichia coliFluoridesGene ExpressionGene Expression Regulation, FungalGenome, FungalIon TransportMembrane ProteinsMolecular Sequence DataMutationPatch-Clamp TechniquesPhosphorylationPhylogenyProtein FoldingProtein MultimerizationProtein Structure, SecondaryProtein Structure, TertiaryRecombinant ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence AlignmentStatic ElectricityConceptsC-terminal domainSaccharomyces cerevisiae functionsYeast plasma membraneN-terminal domainEffects of mutationsDuplicate proteinsYeast genomeCerevisiae functionsSequence conservationHelix domainLinker helixUbiquitous environmental toxinHomologous domainsImportant residuesFluoride channelsPlasma membraneParticular residuesBiological speciesSimilar mutationsIon channelsContinuous expressionProteinAntiparallel dimerMutationsResidues
2005
Quantitative Modeling of Chloride Conductance in Yeast TRK Potassium Transporters
Rivetta A, Slayman C, Kuroda T. Quantitative Modeling of Chloride Conductance in Yeast TRK Potassium Transporters. Biophysical Journal 2005, 89: 2412-2426. PMID: 16040756, PMCID: PMC1366741, DOI: 10.1529/biophysj.105.066712.Peer-Reviewed Original ResearchConceptsTrk proteinTrk potassium transportersPotassium transportersYeast spheroplastsPlasma membraneCentral poreNegative membrane voltagesProteinActive accumulationChloride conductanceChloride currentsMembrane dielectricMembrane voltageSaccharomycesSpheroplastsFungiPlantsTransportersBacteriaConductanceMembrane slope conductanceHypothetical structural modelStructural modelMembraneSlope conductance
2004
The TRK1 Potassium Transporter Is the Critical Effector for Killing of Candida albicans by the Cationic Protein, Histatin 5*
Baev D, Rivetta A, Vylkova S, Sun JN, Zeng GF, Slayman CL, Edgerton M. The TRK1 Potassium Transporter Is the Critical Effector for Killing of Candida albicans by the Cationic Protein, Histatin 5*. Journal Of Biological Chemistry 2004, 279: 55060-55072. PMID: 15485849, DOI: 10.1074/jbc.m411031200.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAdenosine TriphosphateAllelesAnionsAntifungal AgentsAntimicrobial Cationic PeptidesBlotting, WesternCandida albicansCation Transport ProteinsCationsCell MembraneCell SeparationChloride ChannelsChloridesCytoplasmDNA PrimersDNA, ComplementaryDose-Response Relationship, DrugElectrophysiologyEscherichia coliFlow CytometryGene DeletionGenetic Complementation TestHistatinsHistidineModels, ChemicalModels, GeneticOligonucleotidesOpen Reading FramesPatch-Clamp TechniquesPlasmidsPotassiumProtease InhibitorsProtein BindingProtein Structure, TertiaryReverse Transcriptase Polymerase Chain ReactionRNARubidiumSaccharomyces cerevisiae ProteinsSalivary Proteins and PeptidesTime FactorsConceptsHst 5Hst 5 toxicityCritical effectorWild-type cellsTrk1 potassium transporterC. albicansPotassium transportersDiploid organismsOverexpression strainSingle copyCytoplasmic sequestrationPlasma membraneHistatin 5Essential pathwayPathogenic fungiCandida albicansAnion channel inhibitorsGenesTrk1pProteinATP lossChloride conductanceSmall moleculesEffectorsPossible role
1998
Electrophysiology in the eukaryotic model cell Saccharomyces cerevisiae
Bertl A, Bihler H, Kettner C, Slayman CL. Electrophysiology in the eukaryotic model cell Saccharomyces cerevisiae. Pflügers Archiv - European Journal Of Physiology 1998, 436: 999-1013. PMID: 9799419, DOI: 10.1007/s004240050735.Peer-Reviewed Original ResearchConceptsYeast plasma membranePlasma membraneMembrane proteinsYeast membranesFunctional analysisHeterologous membrane proteinsFacile genetic manipulationDetailed functional analysisPreliminary functional analysisYeast vacuolar membraneGigaseal formationHeterologous genesYeast vacuoleCytoplasmic proteinsVacuolar membraneFungal proteinsGenetic manipulationSpecific proteinsPolyploid strainsSaccharomycesNative proteinYeastProtoplastsIon channelsProtein