2010
Interaction between cartilage oligomeric matrix protein and extracellular matrix protein 1 mediates endochondral bone growth
Kong L, Tian Q, Guo F, Mucignat M, Perris R, Sercu S, Merregaert J, Di Cesare P, Liu C. Interaction between cartilage oligomeric matrix protein and extracellular matrix protein 1 mediates endochondral bone growth. Matrix Biology 2010, 29: 276-286. PMID: 20138147, PMCID: PMC2862898, DOI: 10.1016/j.matbio.2010.01.007.Peer-Reviewed Original ResearchConceptsExtracellular matrix protein 1Matrix protein 1EGF domainsFunctional genetic screensProtein 1Endochondral bone growthGenetic screenEndochondral bone formationBiological functionsC-terminusMatrix proteinsMatrix mineralizationBiological significanceCartilage oligomeric matrix proteinOligomeric matrix proteinChondrocyte hypertrophyFirst evidenceBone growthInhibitionGrowthColocalizeBone formationDomainProteinInteraction
2008
Mediation of Chondrogenic and Osteogenic Differentiation by an Interferon-Inducible p202 Protein
Kong L, Liu C. Mediation of Chondrogenic and Osteogenic Differentiation by an Interferon-Inducible p202 Protein. Cellular And Molecular Life Sciences 2008, 65: 3494-3506. PMID: 18791844, PMCID: PMC11131663, DOI: 10.1007/s00018-008-8342-5.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell LineChondrocytesChondrogenesisFeedback, PhysiologicalGene Expression Regulation, DevelopmentalGene Knockdown TechniquesGenes, ReporterGrowth PlateIntracellular Signaling Peptides and ProteinsMiceMice, Inbred C3HMice, KnockoutMice, TransgenicOsteoblastsOsteogenesisParathyroid Hormone-Related ProteinPluripotent Stem CellsRNA, Small InterferingSmad ProteinsConceptsParathyroid hormone-related peptideExpression of PTHrPHormone-related peptideCourse of osteogenesisGrowth plate chondrocytesInterferon-inducible proteinMolecular mechanism studiesInterferon-inducible p200 familyImportant mediatorP202 proteinOsteogenic differentiationSiRNA approachMouse embryosP202 expressionChondrocyte differentiationPositive feedback loopSmad transcription factorsTransgenic mouse embryosOsteoblast differentiationDifferential expressionExpressionC3H10T1/2 cellsC2C12 cellsDifferentiationCellsCbfa1-dependent expression of an interferon-inducible p204 protein is required for chondrocyte differentiation
Zhang Y, Kong L, Carlson C, Liu C. Cbfa1-dependent expression of an interferon-inducible p204 protein is required for chondrocyte differentiation. Cell Death & Differentiation 2008, 15: 1760-1771. PMID: 18636074, DOI: 10.1038/cdd.2008.112.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBone Morphogenetic Protein 2Cell DifferentiationChondrocytesChondrogenesisCollagen Type XCore Binding Factor Alpha 1 SubunitEmbryo, MammalianGrowth PlateHedgehog ProteinsHypertrophyImmunohistochemistryMiceModels, BiologicalMolecular Sequence DataNuclear ProteinsParathyroid Hormone-Related ProteinPhosphoproteinsPromoter Regions, GeneticProtein BindingRNA, Small InterferingSignal TransductionSOXD Transcription FactorsTranscriptional ActivationConceptsChondrocyte hypertrophyChondrocyte differentiationMatrix metalloproteinase-13Indian hedgehogHypertrophic chondrocyte differentiationGrowth plate chondrocytesMetalloproteinase-13P204 proteinReceptor 1HypertrophyAltered levelsType X collagenAltered expressionEnhanced expressionProminent expressionSiRNA approachOverexpression of p204Collagen XSpecific reporter genesPluripotent C3H10T1/2 cellsX collagenNovel regulatorExpressionCbfa1C3H10T1/2 cells
2007
Cartilage Oligomeric Matrix Protein Associates with Granulin-Epithelin Precursor (GEP) and Potentiates GEP-stimulated Chondrocyte Proliferation*
Xu K, Zhang Y, Ilalov K, Carlson C, Feng J, Di Cesare P, Liu C. Cartilage Oligomeric Matrix Protein Associates with Granulin-Epithelin Precursor (GEP) and Potentiates GEP-stimulated Chondrocyte Proliferation*. Journal Of Biological Chemistry 2007, 282: 11347-11355. PMID: 17307734, DOI: 10.1074/jbc.m608744200.Peer-Reviewed Original ResearchConceptsGranulin-epithelin precursorCartilage oligomeric matrix proteinMutant cartilage oligomeric matrix proteinTwo-hybrid systemCo-immunoprecipitation assaysFunctional genetic screensChondrocyte proliferationHuman cartilage oligomeric matrix proteinGenetic screenRat chondrosarcoma cellsProtein associatesGrowth factorRepeat domainBiological functionsFunctional domainsMouse embryosMatrix proteinsAnti-COMP antibodiesChondrosarcoma cellsOligomeric matrix proteinAutocrine growth factorMultiple epiphyseal dysplasiaPericellular matrixPrimary human chondrocytesRepeat units