2020
In Vitro Physical and Functional Interaction Assays to Examine the Binding of Progranulin Derivative Atsttrin to TNFR2 and Its Anti-TNFα Activity
Fu W, Hettinghouse A, Liu C. In Vitro Physical and Functional Interaction Assays to Examine the Binding of Progranulin Derivative Atsttrin to TNFR2 and Its Anti-TNFα Activity. Methods In Molecular Biology 2020, 2248: 109-119. PMID: 33185871, PMCID: PMC8112733, DOI: 10.1007/978-1-0716-1130-2_8.Peer-Reviewed Original ResearchConceptsAnti-TNFα activityAutoimmune diseasesTartrate-resistant acid phosphatase (TRAP) stainingAnti-TNFα therapyCollagen-induced arthritisInflammatory disease modelsGood therapeutic effectAcid phosphatase stainingGrowth factor-like moleculesTNF inhibitorsTherapeutic effectTNFα activityProgranulinFunctional inhibitionTNFR2AtsttrinDisease modelsPhosphatase stainingTNFRTNFαDiseaseInhibitionCritical roleDirect bindingHigh affinity
2009
Cartilage Oligomeric Matrix Protein Maintains the Contractile Phenotype of Vascular Smooth Muscle Cells by Interacting With &agr;7&bgr;1 Integrin
Wang L, Zheng J, Du Y, Huang Y, Li J, Liu B, Liu C, Zhu Y, Gao Y, Xu Q, Kong W, Wang X. Cartilage Oligomeric Matrix Protein Maintains the Contractile Phenotype of Vascular Smooth Muscle Cells by Interacting With &agr;7&bgr;1 Integrin. Circulation Research 2009, 106: 514-525. PMID: 20019333, DOI: 10.1161/circresaha.109.202762.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsAntigens, CDAorta, ThoracicBecaplerminCarotid Artery InjuriesCatheterizationCell DifferentiationCells, CulturedExtracellular MatrixExtracellular Matrix ProteinsGene Knockdown TechniquesGenetic VectorsGlycoproteinsIntegrin alpha ChainsIntegrinsMaleMatrilin ProteinsMuscle ContractionMuscle ProteinsMuscle, Smooth, VascularMyocytes, Smooth MusclePhenotypePlatelet-Derived Growth FactorProtein Interaction MappingProto-Oncogene Proteins c-sisRatsRats, Sprague-DawleyRecombinant Fusion ProteinsRNA, Small InterferingConceptsVascular smooth muscle cellsCartilage oligomeric matrix proteinEffects of COMPContractile phenotypeFocal adhesion assemblyVSMC differentiation marker genesAdenoviral overexpressionSmooth muscle cellsNormal vascular smooth muscle cellsVSMC phenotype switchingDifferentiation marker genesActin fiber organizationMuscle cellsAdhesion assemblyVascular extracellular matrixVSMC contractile phenotypeMarker genesDifferentiated stateRat vascular smooth muscle cellsPhenotype switchingPlatelet-derived growthVSMC adhesionMatrix proteinsVSMC dedifferentiationSmall interfering