2015
Young, Proliferative Thymic Epithelial Cells Engraft and Function in Aging Thymuses
Kim M, Miller C, Shadrach J, Wagers A, Serwold T. Young, Proliferative Thymic Epithelial Cells Engraft and Function in Aging Thymuses. The Journal Of Immunology 2015, 194: 4784-4795. PMID: 25870244, PMCID: PMC4481326, DOI: 10.4049/jimmunol.1403158.Peer-Reviewed Original ResearchConceptsThymic epithelial cellsAge-related thymic involutionMolecular processesColony-forming assayAged thymusCell-intrinsic changesThymic growthProliferative capacityEpithelial cellsIn vitro colony-forming assayIncreased T cell productionT-cell recoveryT-cell lymphopeniaBone marrow transplantationT cell production
2014
Diminished Schwann Cell Repair Responses Underlie Age-Associated Impaired Axonal Regeneration
Painter M, Lutz A, Cheng Y, Latremoliere A, Duong K, Miller C, Posada S, Cobos E, Zhang A, Wagers A, Havton L, Barres B, Omura T, Woolf C. Diminished Schwann Cell Repair Responses Underlie Age-Associated Impaired Axonal Regeneration. Neuron 2014, 83: 331-343. PMID: 25033179, PMCID: PMC4106408, DOI: 10.1016/j.neuron.2014.06.016.Peer-Reviewed Original ResearchConceptsImpairment of functional recoverySchwann cellsSensory neurons in vitroFunctional recoveryMyelin clearanceNeurons in vitroIntrinsic growth capacityTransplantation in vivoPeripheral nervous systemSchwann cell plasticityNerve injuryMacrophage recruitmentAge-associated declineNerve graftsSciatic nerveCell plasticityRegenerative capacityAxonal regenerationNervous systemMolecular interrogationNerveTranscriptional programsInjuryAgeClearanceRestoring Systemic GDF11 Levels Reverses Age-Related Dysfunction in Mouse Skeletal Muscle
Sinha M, Jang Y, Oh J, Khong D, Wu E, Manohar R, Miller C, Regalado S, Loffredo F, Pancoast J, Hirshman M, Lebowitz J, Shadrach J, Cerletti M, Kim M, Serwold T, Goodyear L, Rosner B, Lee R, Wagers A. Restoring Systemic GDF11 Levels Reverses Age-Related Dysfunction in Mouse Skeletal Muscle. Science 2014, 344: 649-652. PMID: 24797481, PMCID: PMC4104429, DOI: 10.1126/science.1251152.Peer-Reviewed Original ResearchConceptsGDF11 levelsSystemic delivery of recombinant proteinsAged miceSkeletal muscleStem cell dysfunctionAged muscle stem cellsDelivery of recombinant proteinsMuscle stem cellsGrowth differentiation factor 11Differentiation factor 11Parabiosis experimentsSystemic deliveryMouse skeletal muscleImpaired regenerationSatellite cellsCell dysfunctionExercise capacityHeterochronic parabiosisYoung bloodStem cellsFactor 11Regenerative functionMiceMuscle agingRejuvenation factorVascular and Neurogenic Rejuvenation of the Aging Mouse Brain by Young Systemic Factors
Katsimpardi L, Litterman N, Schein P, Miller C, Loffredo F, Wojtkiewicz G, Chen J, Lee R, Wagers A, Rubin L. Vascular and Neurogenic Rejuvenation of the Aging Mouse Brain by Young Systemic Factors. Science 2014, 344: 630-634. PMID: 24797482, PMCID: PMC4123747, DOI: 10.1126/science.1251141.Peer-Reviewed Original ResearchConceptsAssociated with reduced blood flowImproved olfactory discriminationAdult central nervous systemDecline of neurogenesisAged mouse brainAge-related declineCentral nervous systemNegative effects of agingNeural stem cellsOlfactory discriminationAge-related decline of neurogenesisCognitive functionAged miceIncreased neurogenesisNeural stem cell behaviorVascular remodelingSecreted factorsEffects of ageEnhanced neurogenesisYoung bloodStem cellsStem cell behaviorBlood flowNeurogenic nicheNervous system
2013
Growth Differentiation Factor 11 Is a Circulating Factor that Reverses Age-Related Cardiac Hypertrophy
Loffredo F, Steinhauser M, Jay S, Gannon J, Pancoast J, Yalamanchi P, Sinha M, Dall’Osso C, Khong D, Shadrach J, Miller C, Singer B, Stewart A, Psychogios N, Gerszten R, Hartigan A, Kim M, Serwold T, Wagers A, Lee R. Growth Differentiation Factor 11 Is a Circulating Factor that Reverses Age-Related Cardiac Hypertrophy. Cell 2013, 153: 828-839. PMID: 23663781, PMCID: PMC3677132, DOI: 10.1016/j.cell.2013.04.015.Peer-Reviewed Original ResearchConceptsCardiac hypertrophyYoung miceOld miceCirculating factorsTreatment of old miceAge-related hypertrophyReduced cardiomyocyte sizeGrowth differentiation factor 11Aptamer-based proteomicsDifferentiation factor 11Blood-borne factorsReverse age-related cardiac hypertrophyCardiomyocyte sizeHeart failureSurgical techniqueHypertrophyCardiac agingHeterochronic parabiosisTherapeutic opportunitiesTGF-bFactor 11Influence of circulation factorsBehavioral effectsMiceMolecular remodeling