2000
A new twist in trypanosome RNA metabolism: cis-splicing of pre-mRNA.
Mair G, Shi H, Li H, Djikeng A, Aviles H, Bishop J, Falcone F, Gavrilescu C, Montgomery J, Santori M, Stern L, Wang Z, Ullu E, Tschudi C. A new twist in trypanosome RNA metabolism: cis-splicing of pre-mRNA. RNA 2000, 6: 163-9. PMID: 10688355, PMCID: PMC1369902, DOI: 10.1017/s135583820099229x.Peer-Reviewed Original ResearchConceptsCis-spliced intronsSplice siteGT/AG ruleSmall intergenic regionAncient acquisitionGene arrangementTrypanosome genomeEukaryotic cellsRNA metabolismIntergenic regionIntron removalLeader sequenceTrypanosoma bruceiIntronsNucleotide positionsAG dinucleotideEuglena gracilisTrypanosoma cruziPolymerase geneIdentical positionsPoint mutationsRecent discoveryPAP mRNAGenesMRNA
1998
Exonic Sequences in the 5′ Untranslated Region of α-Tubulin mRNA Modulate trans Splicing in Trypanosoma brucei
López-Estraño C, Tschudi C, Ullu E. Exonic Sequences in the 5′ Untranslated Region of α-Tubulin mRNA Modulate trans Splicing in Trypanosoma brucei. Molecular And Cellular Biology 1998, 18: 4620-4628. PMID: 9671472, PMCID: PMC109048, DOI: 10.1128/mcb.18.8.4620.Peer-Reviewed Original Research
1993
Temporal order of RNA-processing reactions in trypanosomes: rapid trans splicing precedes polyadenylation of newly synthesized tubulin transcripts.
Ullu E, Matthews K, Tschudi C. Temporal order of RNA-processing reactions in trypanosomes: rapid trans splicing precedes polyadenylation of newly synthesized tubulin transcripts. Molecular And Cellular Biology 1993, 13: 720-725. PMID: 8417363, PMCID: PMC358950, DOI: 10.1128/mcb.13.1.720.Peer-Reviewed Original ResearchConceptsRNA-processing reactionsTrans splicingPre-mRNASplice sitePolycistronic transcription unitsSplice acceptor siteTrypanosome genesMRNA biogenesisTranscription unitTrypanosome mRNAsLeader RNATubulin transcriptsSplicingAlpha-tubulinPolyadenylationMRNA synthesisAcceptor sitesTrypanosomesTemporal orderMRNABiogenesisGenesTranscriptsRNAOrganisms
1990
Permeable trypanosome cells as a model system for transceiption and trans-splicing
Ullu E, Tschudi C. Permeable trypanosome cells as a model system for transceiption and trans-splicing. Nucleic Acids Research 1990, 18: 3319-3326. PMID: 2356121, PMCID: PMC330939, DOI: 10.1093/nar/18.11.3319.Peer-Reviewed Original ResearchConceptsAlpha-tubulin RNASL RNAFree linear moleculesHigh molecular weight RNAMolecular weight RNARNA speciesLeader RNATrypanosome cellsDifferent genesWeight RNARNATranscriptionModel systemCritical rolePermeable cellsConcentrations of monovalentIntronsCellsGenesLesser extentHigh potassium concentrationSpeciesTrypanosomaMgCl2 concentrationLinear moleculesConserved sequences in the U2 snRNA-encoding genes of Kinetoplastida do not include the putative branchpoint recognition region
Tschudi C, Williams S, Ullu E. Conserved sequences in the U2 snRNA-encoding genes of Kinetoplastida do not include the putative branchpoint recognition region. Gene 1990, 91: 71-77. PMID: 2401409, DOI: 10.1016/0378-1119(90)90164-m.Peer-Reviewed Original ResearchConceptsU2 small nuclear RNASmall nuclear RNAU2 genesEvolutionary conservationOrder KinetoplastidaConserved sequencesNuclear RNASequence variationFlanking regionsNuclear extractsRecognition sequenceKinetoplastidaUpstream elementRecognition regionGenesRNACorresponding sequenceLeishmania mexicana amazonensisSequenceT. congolenseTrypanosoma congolenseBiosynthesisNucleotidesProteinCongolense
1988
Polygene transcripts are precursors to calmodulin mRNAs in trypanosomes.
Tschudi C, Ullu E. Polygene transcripts are precursors to calmodulin mRNAs in trypanosomes. The EMBO Journal 1988, 7: 455-463. PMID: 3366120, PMCID: PMC454342, DOI: 10.1002/j.1460-2075.1988.tb02833.x.Peer-Reviewed Original ResearchConceptsCalmodulin mRNACultured procyclic trypanosomesAbundant RNA speciesSteady-state RNAPulse-labeled RNAAuthentic precursorIntergenic regionRNA speciesProcyclic trypanosomesCalmodulin genePolyadenylation sitesAfrican trypanosomesHybridization probesTranscriptsStreptavidin-agaroseDiscrete endsSmall familyTrypanosomesMRNAGenesRNASpeciesCalmodulinTrypanosoma brucei gambienseBrucei gambiense
1986
The U2 RNA analogue of Trypanosoma brucei gambiense: implications for a splicing mechanism in trypanosomes
Tschudi C, Richards F, Ullu E. The U2 RNA analogue of Trypanosoma brucei gambiense: implications for a splicing mechanism in trypanosomes. Nucleic Acids Research 1986, 14: 8893-8903. PMID: 3786140, PMCID: PMC311918, DOI: 10.1093/nar/14.22.8893.Peer-Reviewed Original ResearchConceptsU2 RNASpliced leader RNASingle-copy geneSignificant sequence homologyMaturation of mRNAMammalian counterpartsCopy geneU RNALeader RNASplicing mechanismSequence homologySecondary structureRNATrypanosomesGenesRNA analogSplicingHomologyTrypanosoma brucei gambiensePossible interactionsMRNABrucei gambienseMaturation
1985
Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5' leader sequence.
Tschudi C, Young A, Ruben L, Patton C, Richards F. Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5' leader sequence. Proceedings Of The National Academy Of Sciences Of The United States Of America 1985, 82: 3998-4002. PMID: 3858856, PMCID: PMC397921, DOI: 10.1073/pnas.82.12.3998.Peer-Reviewed Original Research
1984
Alu sequences are processed 7SL RNA genes
Ullu E, Tschudi C. Alu sequences are processed 7SL RNA genes. Nature 1984, 312: 171-172. PMID: 6209580, DOI: 10.1038/312171a0.Peer-Reviewed Original ResearchConceptsRNA genesAlu sequencesMiddle repetitive DNA sequencesAbundant cytoplasmic RNARepetitive DNA sequencesHuman 7SL RNAInsect sequencesAlu domainMammalian radiationDrosophila melanogasterEvolutionary distanceXenopus sequenceAbundant familyDNA sequencesNucleotide sequenceCDNA copyCytoplasmic RNAPair sequencePrimary structureProtein secretionRNAGenesHuman counterpartSequenceSignificant block