2024
B-243 Validating a highly automated, rapid turnaround urine biomarker assay for diagnosis of acute interstitial nephritis
Obeid W, Moledina D, Parikh C. B-243 Validating a highly automated, rapid turnaround urine biomarker assay for diagnosis of acute interstitial nephritis. Clinical Chemistry 2024, 70: hvae106.600. DOI: 10.1093/clinchem/hvae106.600.Peer-Reviewed Original ResearchAcute interstitial nephritisDiagnosis of acute interstitial nephritisTNF-aMesoscale DiscoveryCXCL-9Urinary tumor necrosis factor-alphaInterstitial nephritisUrine biomarker assayTumor necrosis factor-alphaNecrosis factor-alphaSpike-recovery analysisFactor-alphaPotential diagnostic biomarkersLevels of QCBiomarker assaysDiagnostic biomarkersAssay diluentDilution linearityNephritisAverage recoverySpiking volumeHospital settingLigand 9UrineDiagnosis
2023
Identification and validation of urinary CXCL9 as a biomarker for diagnosis of acute interstitial nephritis
Moledina D, Obeid W, Smith R, Rosales I, Sise M, Moeckel G, Kashgarian M, Kuperman M, Campbell K, Lefferts S, Meliambro K, Bitzer M, Perazella M, Luciano R, Pober J, Cantley L, Colvin R, Wilson F, Parikh C. Identification and validation of urinary CXCL9 as a biomarker for diagnosis of acute interstitial nephritis. Journal Of Clinical Investigation 2023, 133: e168950. PMID: 37395276, PMCID: PMC10313360, DOI: 10.1172/jci168950.Peer-Reviewed Original ResearchConceptsUrinary CXCL9External validation cohortValidation cohortControl groupAIN diagnosisDiscovery cohortKidney tissueDiagnostic biomarkersAcute interstitial nephritisCXCL9 mRNA expressionAcute kidney injuryBiopsy-confirmed diagnosisAvailable clinical testsNational InstituteKidney injuryTubulointerstitial nephritisInterstitial nephritisKidney biopsyHistological confirmationHistological diagnosisTreatment optionsLymphocyte chemotaxisCXCL9MRNA expression differencesPatients