2011
Development of optimized AAV3 serotype vectors: mechanism of high-efficiency transduction of human liver cancer cells
Cheng B, Ling C, Dai Y, Lu Y, Glushakova L, Gee S, McGoogan K, Aslanidi G, Park M, Stacpoole P, Siemann D, Liu C, Srivastava A, Ling C. Development of optimized AAV3 serotype vectors: mechanism of high-efficiency transduction of human liver cancer cells. Gene Therapy 2011, 19: 375-384. PMID: 21776025, PMCID: PMC3519243, DOI: 10.1038/gt.2011.105.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBreast NeoplasmsCarcinoma, HepatocellularCell Line, TumorDependovirusFemaleGenetic VectorsHumansLiver NeoplasmsMiceMutagenesis, Site-DirectedProteasome InhibitorsProtein Structure, TertiaryProto-Oncogene Proteins c-metTransduction, GeneticTransplantation, HeterologousTyrosineVirus IntegrationConceptsHigh-efficiency transductionAAV3 vectorsTransduction efficiencySerotype vectorsSurface-exposed tyrosinePotential gene therapyHuman hepatocyte growth factor receptorHuman liver cancer cellsGene therapyLiver cancer cellsTransgene expressionVector entryAAV3 capsidsDirect intratumoral injectionAAV3Tail vein injectionLiver tumor cellsIntratumoral injectionCancer cellsEfficiencyAdenoVectorIntracellular kinase domainVirus serotypes
1994
Identification of factor-binding sites in the duck hepatitis B virus enhancer and in vivo effects of enhancer mutations.
Liu C, Mason W, Burch J. Identification of factor-binding sites in the duck hepatitis B virus enhancer and in vivo effects of enhancer mutations. Journal Of Virology 1994, 68: 2286-96. PMID: 8139013, PMCID: PMC236704, DOI: 10.1128/jvi.68.4.2286-2296.1994.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell NucleusCells, CulturedDNA Mutational AnalysisDNA-Binding ProteinsDNA, ViralDucksEnhancer Elements, GeneticHepatitis B Virus, DuckHepatocyte Nuclear Factor 1Hepatocyte Nuclear Factor 1-betaLiverMolecular Sequence DataMutagenesis, Site-DirectedNuclear ProteinsPancreasProtein BindingTranscription FactorsConceptsHepatitis B virus enhancerVirus replicationDuck hepatitis B virus replicationHepatitis B virus replicationB virus replicationHepatitis B virusCore gene promoterLMH chicken hepatoma cellsHuman growth hormoneB virusLiver-enriched transcription factorsTwo- to fourfoldVivo effectsIntact viral genomeGrowth hormoneVirus enhancerPrimary hepatocyte culturesShort-term assaysProductive infectionSignificant inhibitionVirus RNA synthesisVirusCell linesHepatoma cellsFactor-binding sites