2019
T cell–derived interferon-γ programs stem cell death in immune-mediated intestinal damage
Takashima S, Martin M, Jansen S, Fu Y, Bos J, Chandra D, O'Connor M, Mertelsmann A, Vinci P, Kuttiyara J, Devlin S, Middendorp S, Calafiore M, Egorova A, Kleppe M, Lo Y, Shroyer N, Cheng E, Levine R, Liu C, Kolesnick R, Lindemans C, Hanash A. T cell–derived interferon-γ programs stem cell death in immune-mediated intestinal damage. Science Immunology 2019, 4 PMID: 31811055, PMCID: PMC7239329, DOI: 10.1126/sciimmunol.aay8556.Peer-Reviewed Original ResearchConceptsBone marrow transplantationIntestinal stem cellsDonor T cellsStem cell compartmentT cellsStem cell deathEpithelial culturesAllogeneic bone marrow transplantationDysregulated T cell activationT cell-mediated pathologyInhibition of JAK signalingStem cellsImmune-mediated damageActivated T cellsT cell activationCell compartmentCell deathTissue stem cellsPaneth cell nicheIFNg productionMarrow transplantationIntestinal immunopathologyInterferon-gHealthy miceStem cell coloniesThe role of necroptosis in cancer biology and therapy
Gong Y, Fan Z, Luo G, Yang C, Huang Q, Fan K, Cheng H, Jin K, Ni Q, Yu X, Liu C. The role of necroptosis in cancer biology and therapy. Molecular Cancer 2019, 18: 100. PMID: 31122251, PMCID: PMC6532150, DOI: 10.1186/s12943-019-1029-8.Peer-Reviewed Original ResearchConceptsMixed lineage kinase domain-likeReceptor-interacting protein 1Apoptosis resistanceNecrotic cell death modalitiesCell deathMode of programmed cell deathCaspase-independent fashionCancer metastasisTumor progressionLineage kinase domain-likeCancer cellsInduce cancer cell deathCell death modalitiesCell death mechanismsCancer cell deathCancer immunity regulationImmunosuppressive tumor microenvironmentPrognosis of cancer patientsApoptotic pathwayAdaptive immune responsesDeath modalitiesPromote oncogenesisNecroptotic pathwayDeath mechanismsMetabolic signals
2009
AAV3-mediated transfer and expression of the pyruvate dehydrogenase E1 alpha subunit gene causes metabolic remodeling and apoptosis of human liver cancer cells
Glushakova L, Lisankie M, Eruslanov E, Ojano-Dirain C, Zolotukhin I, Liu C, Srivastava A, Stacpoole P. AAV3-mediated transfer and expression of the pyruvate dehydrogenase E1 alpha subunit gene causes metabolic remodeling and apoptosis of human liver cancer cells. Molecular Genetics And Metabolism 2009, 98: 289-299. PMID: 19586787, PMCID: PMC2779054, DOI: 10.1016/j.ymgme.2009.05.010.Peer-Reviewed Original ResearchConceptsPyruvate dehydrogenase complexSubunit geneReporter geneWarburg effectCell deathCancer cellsGreen fluorescent reporter geneLiver cancer cellsAlpha-subunit geneFluorescent reporter geneHuman hepatoblastomaE1alpha subunit geneHuH-6 cellsApoptotic cell deathProtein expressionMitochondrial oxidative metabolismHuman alpha-fetoprotein promoterMitochondrial respiratory chain activityBeta-actin promoterRespiratory chain activityChicken beta-actin promoterReactive oxygen speciesExpression systemDehydrogenase complexMetabolic remodeling