2016
Noncoding Subgenomic Flavivirus RNA Is Processed by the Mosquito RNA Interference Machinery and Determines West Nile Virus Transmission by Culex pipiens Mosquitoes
Göertz GP, Fros JJ, Miesen P, Vogels CB, van der Bent ML, Geertsema C, Koenraadt CJ, van Rij RP, van Oers MM, Pijlman GP. Noncoding Subgenomic Flavivirus RNA Is Processed by the Mosquito RNA Interference Machinery and Determines West Nile Virus Transmission by Culex pipiens Mosquitoes. Journal Of Virology 2016, 90: 10145-10159. PMID: 27581979, PMCID: PMC5105652, DOI: 10.1128/jvi.00930-16.Peer-Reviewed Original ResearchConceptsSubgenomic flavivirus RNARNA interference machineryCulex pipiens mosquitoesWest Nile virusInterference machinerySfRNA productionPipiens mosquitoesBiological functionsUntranslated regionNile virusPivotal biological functionsIntrathoracic injectionWild-type WNVRNA deep sequencingKey biological functionsBlood mealFlavivirus RNAMosquito cell linesFlavivirus West Nile virusViral genomic RNAMosquito midgut barrierMutant West Nile virusTick-borne flavivirusesWNV-infected mosquitoesMammalian cells
2015
Comparative Usutu and West Nile virus transmission potential by local Culex pipiens mosquitoes in north-western Europe
Fros JJ, Miesen P, Vogels CB, Gaibani P, Sambri V, Martina BE, Koenraadt CJ, van Rij RP, Vlak JM, Takken W, Pijlman GP. Comparative Usutu and West Nile virus transmission potential by local Culex pipiens mosquitoes in north-western Europe. One Health 2015, 1: 31-36. PMID: 28616462, PMCID: PMC5441354, DOI: 10.1016/j.onehlt.2015.08.002.Peer-Reviewed Original ResearchWest Nile virusUsutu virusMosquito-borne flavivirusHigh mortality rateVirus transmission potentialNeuroinvasive diseaseUSUV transmissionWNV infectionEuropean mosquitoesMortality rateInfected mosquitoesInfection rateMosquito midgutSmall interferingNile virusTransmission potentialVirusEntire viral genomeTransmission dynamicsVector competenceCulex pipiens mosquitoesRNA deep sequencingMosquitoesViral genomeSmall RNA deep sequencing