2004
WNK4 regulates apical and basolateral Cl– flux in extrarenal epithelia
Kahle KT, Gimenez I, Hassan H, Wilson FH, Wong RD, Forbush B, Aronson PS, Lifton RP. WNK4 regulates apical and basolateral Cl– flux in extrarenal epithelia. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 2064-2069. PMID: 14769928, PMCID: PMC357052, DOI: 10.1073/pnas.0308434100.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCarrier ProteinsCell PolarityChloridesEpitheliumHumansImmunohistochemistryIon TransportKidneyMembrane ProteinsMembrane Transport ProteinsMiceOocytesProtein Serine-Threonine KinasesRNA, MessengerSodium-Potassium-Chloride SymportersSolute Carrier Family 12, Member 2Sulfate TransportersXenopus laevisConceptsCl fluxBlood-brain barrierUnrelated ion channelsActivity of mediatorsWNK4 mRNABile ductPancreatic ductExtrarenal expressionExtrarenal tissuesCl(-) handlingPseudohypoaldosteronism type IIChannel ROMKNaCl reabsorptionSerine-threonine kinase WNK4Specialized endotheliumExchanger SLC26A6NaCl cotransporterWNK4 effectsColonic cryptsEpitheliumVariable inhibitionSweat ductsTight junctionsKidneyElectrolyte flux
1996
Immunocytochemical studies of the Na-K-Cl cotransporter of shark kidney
Biemesderfer D, Payne J, Lytle C, Forbush B. Immunocytochemical studies of the Na-K-Cl cotransporter of shark kidney. American Journal Of Physiology 1996, 270: f927-f936. PMID: 8764311, DOI: 10.1152/ajprenal.1996.270.6.f927.Peer-Reviewed Original ResearchConceptsNa-K-Cl cotransporterNa-K-ClBasolateral membraneCotransporter proteinBasolateral membranes of proximal tubulesNa-K-Cl cotransport proteinShark kidneyBasolateral plasma membraneDistal tubule segmentsApical plasma membraneShark rectal glandDistal nephronReabsorptive epitheliaNaCl reabsorptionPlasma membraneWestern blot analysisTubule segmentsProximal tubulesDiluting segmentWeak stainingShark Squalus acanthiasPresence of multiple isoformsDogfish shark Squalus acanthiasCotransporterMonoclonal antibodies