1995
Cloning, embryonic expression, and alternative splicing of a murine kidney-specific Na-K-Cl cotransporter
Igarashi P, Heuvel G, Payne J, Forbush B. Cloning, embryonic expression, and alternative splicing of a murine kidney-specific Na-K-Cl cotransporter. American Journal Of Physiology 1995, 269: f405-f418. PMID: 7573490, DOI: 10.1152/ajprenal.1995.269.3.f405.Peer-Reviewed Original ResearchConceptsNa-K-Cl cotransporterExpression of NKCC2In situ hybridizationCassette exonsRenal Na-K-Cl cotransporterOuter medullaAscending limbAlternative splicingInner stripe of outer medullaPotential sites of phosphorylationAdenosine 3',5'-cyclic monophosphate-dependent protein kinaseDays post coitusFull-length cDNAAnchored polymerase chain reactionSites of phosphorylationConsensus splice donorLoop of HenleS-shaped bodiesProtein kinase CNorthern blot analysisPattern of expressionGenomic clonesNKCC2Outer stripeProtein sequencesMolecular characterization of the epithelial NaKCl cotransporter isoforms
Payne J, Forbush B. Molecular characterization of the epithelial NaKCl cotransporter isoforms. Current Opinion In Cell Biology 1995, 7: 493-503. PMID: 7495568, DOI: 10.1016/0955-0674(95)80005-0.Peer-Reviewed Original Research
1983
[3H]bumetanide binding to membranes isolated from dog kidney outer medulla. Relationship to the Na,K,Cl co-transport system.
Forbush B, Palfrey H. [3H]bumetanide binding to membranes isolated from dog kidney outer medulla. Relationship to the Na,K,Cl co-transport system. Journal Of Biological Chemistry 1983, 258: 11787-11792. PMID: 6619143, DOI: 10.1016/s0021-9258(17)44299-2.Peer-Reviewed Original ResearchConceptsOuter medullaCl- co-transport systemDog kidney outer medullaCl- cotransport systemLoop of HenleCo-transport systemLoop diureticsBasolateral membraneKidney outer medullaProximal tubulesAscending limbLuminal membraneCotransport systemKidney cortexMedullaKidney membranesBrush borderCo-transportCrude membranesKidneyDog kidneyBinding to membranesBinding activityGradient centrifugation