The Na-K-Cl cotransport protein of shark rectal gland. II. Regulation by direct phosphorylation.
Lytle C, Forbush B. The Na-K-Cl cotransport protein of shark rectal gland. II. Regulation by direct phosphorylation. Journal Of Biological Chemistry 1992, 267: 25438-25443. PMID: 1334094, DOI: 10.1016/s0021-9258(19)74060-5.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCarrier ProteinsChromatography, High Pressure LiquidCyclic AMPDogfishHypertonic SolutionsIsoquinolinesKineticsMembrane ProteinsMolecular Sequence DataPeptide FragmentsPhosphorylationProtein Kinase InhibitorsRectumSebaceous GlandsSodium-Potassium-Chloride SymportersSulfanilamidesThionucleotidesConceptsNa-K-Cl cotransport proteinNa-K-ClCotransporter proteinShark rectal glandCell shrinkageCAMP-dependent secretagoguesRectal gland tubulesOsmotically induced changesCotransporter phosphorylationCotransport activityKinase inhibitorsProtein kinase inhibitorsCotransporterPhosphorylation stateRectal glandActive stateH-8K-252aTubulesGlandPhosphorylationCDNA analysisCellsIncreased 5Protein phosphorylationNa-K-Cl cotransport in the shark rectal gland. II. Regulation in isolated tubules
Lytle C, Forbush B. Na-K-Cl cotransport in the shark rectal gland. II. Regulation in isolated tubules. American Journal Of Physiology 1992, 262: c1009-c1017. PMID: 1314482, DOI: 10.1152/ajpcell.1992.262.4.c1009.Peer-Reviewed Original ResearchConceptsNa-K-Cl cotransporterNa-K-ClShark rectal glandInhibitor of Na-K-Cl cotransportApical Cl- channelsCell shrinkageEffects of secretagoguesOsmotically induced changesSensitivity to cAMPCl- channelsExtracellular sodiumIsolated tubulesCotransporterHormonal modulationSecretory stimuliSignal transduction pathwaysSecretagoguesRectal glandTubulesRectal gland secretionHypertonicityCell volumeTransduction pathwaysGlandCellsNa-K-Cl cotransport in the shark rectal gland. I. Regulation in the intact perfused gland
Forbush B, Haas M, Lytle C. Na-K-Cl cotransport in the shark rectal gland. I. Regulation in the intact perfused gland. American Journal Of Physiology 1992, 262: c1000-c1008. PMID: 1566806, DOI: 10.1152/ajpcell.1992.262.4.c1000.Peer-Reviewed Original ResearchConceptsNa-K-ClNa-K-Cl cotransport systemNa-K-Cl cotransporterVasoactive intestinal peptideCotransport systemVIP-stimulated increasesStimulation of secretionShark rectal glandK exitBasolateral membranePerfused glandShark Squalus acanthiasIntestinal peptideArterial-venous differencesDogfish shark Squalus acanthiasIntact glandsPerfusion solutionPerfusion periodBasal statePerfusionGlandInvestigate regulationRectal glandRinger's solutionTime course