Large-scale identification of chemically induced mutations in Drosophila melanogaster
Haelterman NA, Jiang L, Li Y, Bayat V, Sandoval H, Ugur B, Tan KL, Zhang K, Bei D, Xiong B, Charng WL, Busby T, Jawaid A, David G, Jaiswal M, Venken KJ, Yamamoto S, Chen R, Bellen HJ. Large-scale identification of chemically induced mutations in Drosophila melanogaster. Genome Research 2014, 24: 1707-1718. PMID: 25258387, PMCID: PMC4199363, DOI: 10.1101/gr.174615.114.Peer-Reviewed Original ResearchConceptsWhole-genome sequencingDrosophila X chromosomeThousands of polymorphismsEukaryotic model organismForward genetic screenChemical mutagenesis screenFunctions of thousandsPhenotype of interestLarge-scale identificationTime-consuming identificationHundreds of strainsSingle nucleotide variantsGenetic screenEssential genesDrosophila melanogasterModel organismsMutagenesis screenRescue lethalityX chromosomeMutant strainCandidate mutationsMolecular lesionsLarge duplicationChemical mutagensRough mapping