1996
[16] Purification of yeast RNA polymerase II holoenzymes
Koleske AJ, Chao DM, Young RA. [16] Purification of yeast RNA polymerase II holoenzymes. Methods In Enzymology 1996, 273: 176-184. PMID: 8791611, DOI: 10.1016/s0076-6879(96)73018-5.Peer-Reviewed Original ResearchConceptsRNA polymerase II holoenzymeRNA polymerase IIPolymerase IIYeast RNA polymerase II holoenzymeGeneral transcription factors TBPTranscription factors TBPHigh molecular weight complexesMolecular weight complexesTranscriptional regulationTranscription initiationYeast SaccharomycesTranscription studiesHoloenzymeHoloenzyme preparationWeight complexesTranscriptionLow salt bufferExciting cluesLarge formSalt concentrationTFIIBTFIIEAssaysSaccharomycesPurification
1995
Association of an activator with an RNA polymerase II holoenzyme.
Hengartner CJ, Thompson CM, Zhang J, Chao DM, Liao SM, Koleske AJ, Okamura S, Young RA. Association of an activator with an RNA polymerase II holoenzyme. Genes & Development 1995, 9: 897-910. PMID: 7774808, DOI: 10.1101/gad.9.8.897.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceGenes, FungalHerpes Simplex Virus Protein Vmw65Macromolecular SubstancesMediator ComplexModels, GeneticMolecular Sequence DataMutationPrecipitin TestsProtein BindingRestriction MappingRNA Polymerase IISaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Analysis, DNASuppression, GeneticTranscription FactorsTranscription, GeneticConceptsRNA polymerase II holoenzymeRNA polymerase IISRB proteinsPolymerase IIDomain of VP16General transcription factorsDirect interactionTranscription apparatusTranscriptional activatorMediators of activationTranscription initiationTranscription factorsImmunoprecipitation experimentsHoloenzymeGenesActivatorAffinity columnProteinSubcomplexPromoterVP16BindsInteractionHallmarkActivation
1993
A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast
Thompson C, Koleske A, Chao D, Young R. A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast. Cell 1993, 73: 1361-1375. PMID: 8324825, DOI: 10.1016/0092-8674(93)90362-t.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBinding SitesDNA Mutational AnalysisDNA-Binding ProteinsFungal ProteinsGene Expression RegulationMediator ComplexMolecular Sequence DataMultienzyme ComplexesRecombinant ProteinsRNA Polymerase IISaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTATA-Box Binding ProteinTranscription FactorsTranscription, GeneticConceptsTATA-binding proteinRNA polymerase II carboxy-terminal domainCarboxy-terminal domainMultisubunit complexLarge multisubunit complexFunctional preinitiation complexRNA polymerase IIEfficient transcription initiationTranscription initiation complexSRB proteinsCTD proteinsExtragenic suppressorsCTD functionPolymerase IIPreinitiation complexTranscription initiationInitiation complexComplex bindsTruncation mutationsSRB2Srb5ProteinBiochemical evidenceComplexesSRB4
1992
A novel transcription factor reveals a functional link between the RNA polymerase II CTD and TFIID
Koleske A, Buratowski S, Nonet M, Young R. A novel transcription factor reveals a functional link between the RNA polymerase II CTD and TFIID. Cell 1992, 69: 883-894. PMID: 1591782, DOI: 10.1016/0092-8674(92)90298-q.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceBlotting, WesternChromatography, AffinityFungal ProteinsGenes, SuppressorMediator ComplexMolecular Sequence DataMutationRecombinant Fusion ProteinsRNA Polymerase IISaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription Factor TFIIDTranscription FactorsTranscription, GeneticConceptsCarboxy-terminal domainRNA polymerase II carboxy-terminal domainNovel transcription factorTranscription initiation complexInitiation complexTranscription factorsFunctional linkConditional growth phenotypesTranscription initiation apparatusRNA polymerase IITATA-binding factorDominant suppressorsPolymerase IIGrowth phenotypeTranscription initiationGene expressionAllele specificitySRB2Truncation mutationsSame functionSimilar defectsTFIIDSuppressorEfficient establishmentMechanism of action